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URL: http://www.addgene.org/100093
Proper Citation: RRID:Addgene_100093
Insert Name: 14-3-3 sigma human
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:28931924
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28-his-3C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: The 3C site was introduced immediately prior to 14-3-3 sequence to facilitate purification by subtractive IMAC.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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