Searching the Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
The processing of small nuclear RNA post transcription involves endolytic cleavage of a 3' tail to produce a mature transcript that is incorporated into the spliceosome to regulate RNA splicing. We previously reported in the Caenorhabditis elegans model several novel genetic regulators including those functioning in RNAi processing to be required for snRNA cleavage through a genome-wide RNAi screen using an in vivo snRNA misprocessing reporter. Here, we conducted a forward genetic screen using the mutagen ethyl methanesulfonate to screen for viable mutants that exhibit constitutive snRNA misprocessing. This screen generated three new recessive rde-1 mutant alleles (cww1, cww4, cww9) identified via WGS SNP mapping, which encode the primary Argonaute protein involved in the processing of exogenous RNAi. The three rde-1 alleles failed to complement each other and rde-1(cww1) which contains a premature stop codon in exon 3 also failed to be complemented by the classic rde-1(ne219) allele. We show that the three rde-1 mutants display a varying degree of snRNA misprocessing reporter activation, but are all fully resistant to various RNAi that are known to cause larval arrest or an abnormal vulva phenotype. Thus, the screen has reinforced a connection between RNAi processing and snRNA cleavage and generated mutants that are useful for future studies of the rde-1 Argonaute gene.
Pubmed ID: 40324366
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Cloud platform to be directly integrated in to the industry?s leading sequencing platforms, with no cumbersome and time consuming data transfer steps.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsCaenorhabditis elegans with name Caenorhabditis elegans wild isolate. from WB.
View all literature mentionsCaenorhabditis elegans with name rde-1(ne219) V. from WB.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsWelcome to the Neuroscience Information Framework Project, designed to serve the biomedical research community. NIF maintains the largest searchable collection of neuroscience data, the largest catalog of biomedical resources, and the largest ontology for neuroscience on the web. We welcome all feedback and suggestions and are actively looking for resource providers to make their resources accessible through NIF. Learn about the tools available to help you share your data and discover a dynamic inventory of Web-based neuroscience resources.
