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Spindle bipolarity is critical for genomic integrity. As centrosome number often dictates bipolarity, tight control of centrosome assembly is vital for faithful cell division. The master centrosome regulator ZYG-1/Plk4 plays a pivotal role in this process. In C. elegans, casein kinase II (CK2) negatively regulates centrosome duplication by controlling centrosome-associated ZYG-1 levels. Here, we investigated CK2 as a regulator of ZYG-1 and its impact on centrosome assembly. We show that CK2 phosphorylates ZYG-1 in vitro and physically interacts with ZYG-1 in vivo. Depleting CK2 or blocking ZYG-1 phosphorylation at CK2 target sites leads to centrosome amplification. Non-phosphorylatable ZYG-1 mutants exhibit elevated ZYG-1 levels, leading to increased ZYG-1 and downstream factors at centrosomes, thus driving centrosome amplification. Moreover, inhibiting the 26S proteasome prevents degradation of the phospho-mimetic ZYG-1. Our findings suggest that CK2-dependent phosphorylation of ZYG-1 controls ZYG-1 levels via proteasomal degradation to limit centrosome number.
Pubmed ID: 38034351
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Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsSoftware tool for automated microscope acquisition, device control, and image analysis. Used for integrating dissimilar fluorescent microscope hardware and peripherals into a single custom workstation, while providing all the tools needed to perform analysis of acquired images. Offers user friendly application modules for analysis such as cell signaling, cell counting, and protein expression.
View all literature mentionsCommercial organization for research and development genomics services and technical support to researchers.
View all literature mentionsFully customizable, comprehensive genetic pedigree and clinical data management software including a multi-user relational database with an integrated pedigree drawing component to manage genetic and pedigree data in one database. Manage Pedigrees, Individuals, SNPs, STRs, Samples, Plates, Genotypes and exports to multiple analysis platforms. (entry from Genetic Analysis Software) * LIMS software, providing advanced sample tracking and management (including functionality to generate and record barcodes) and configurable workflows for your specific environment. * Full genotype management gives users the ability to track not only family-based studies, but Whole Genome Association studies containing 1000''s of samples with large arrays.
View all literature mentionsCenter that acquires, maintains, and distributes genetic stocks and information about stocks of the small free-living nematode Caenorhabditis elegans for use by investigators initiating or continuing research on this genetic model organism. A searchable strain database, general information about C. elegans, and links to key Web sites of use to scientists, including WormBase, WormAtlas, and WormBook are available.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs454) II; sas-5(mhs533) V from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs428it25[ZYG-14A:P442L]) II from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs409[ZYG-14D]) II from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs409) II; sas-6(mhs451) IV from WB.
View all literature mentionsVector graphics software to create digital graphics, illustrations, and typography for several types of media: print, web, interactive, video, and mobile.
View all literature mentionsCaenorhabditis elegans with name spd-2(mhs580) I; zyg-1(mhs409) II from WB.
View all literature mentionsCaenorhabditis elegans with name Caenorhabditis elegans wild isolate. from WB.
View all literature mentionsCaenorhabditis elegans with name sas-5(mhs533[SAS-5::V5]) V from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs404it25[ZYG-1S279D:P442L]) II from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs507mhs454[2xMyc::ZYG-14A]) II from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs456mhs482[2xMyc::ZYG-14D]) II from WB.
View all literature mentionsSoftware that detects kinase-specific phosphorylation sites. GPS provides a platform able to perform its prediction based on a group-based phosphorylation scoring algorithm. It allows users to query multiple protein sequences through a batch prediction mode.
View all literature mentionsCaenorhabditis elegans with name ijmSi3 [pJD342/pJD330; ChrI_5mex-5_cls-2reenc::GFP_3tbb-2; cb-unc-119(+)]I; unc-119(ed3) III? ; ltSi1[pOD809/pJE110; Pknl-1::KNL-1reencoded::RFP; cb-unc-119(+)]II; unc-119(ed3) III from WB.
View all literature mentionsSoftware environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs454[ZYG-14A]) II from WB.
View all literature mentionsCaenorhabditis elegans with name spd-2(mhs580) I; zyg-1(mhs454) II from WB.
View all literature mentionsCaenorhabditis elegans with name sas-6(mhs451[Ollas::SAS-6]) IV from WB.
View all literature mentionsCaenorhabditis elegans with name tjIs54; tjIs57. from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(it25[ZYG-1P442L]) II from WB.
View all literature mentionsCaenorhabditis elegans with name kin-3(mhs464) I; zyg-1(mhs456) II from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs456) II; unc-119(ed3) III; bsIs15[pNP99:unc-119(+) tbb1p::mCherry::tbb-2::tbb-2 3UTR from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs456) II; unc-119(ed3) III; bsIs15[pNP99:unc-119(+) tbb1p::mCherry::tbb-2::tbb-2 3UTR from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs456[2xMyc::ZYG-1]) II from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs409) II; sas-5(mhs533) V from WB.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsSoftware tool for redesign and expansion of prediction on kinase specific phosphorylation sites. Machine learning based kinase specific phosphorylation site prediction tool.
View all literature mentionsCaenorhabditis elegans with name spd-2(mhs580[SPD-2::2xHA]) I from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs399it25[ZYG-1S279A:P442L]) II from WB.
View all literature mentionsCaenorhabditis elegans with name kin-3(mhs464) I; zyg-1(mhs456) II from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs389it25[ZYG-14D:P442L]) II from WB.
View all literature mentionsCaenorhabditis elegans with name zyg-1(mhs454) II; sas-6(mhs451) IV from WB.
View all literature mentionsWelcome to the Neuroscience Information Framework Project, designed to serve the biomedical research community. NIF maintains the largest searchable collection of neuroscience data, the largest catalog of biomedical resources, and the largest ontology for neuroscience on the web. We welcome all feedback and suggestions and are actively looking for resource providers to make their resources accessible through NIF. Learn about the tools available to help you share your data and discover a dynamic inventory of Web-based neuroscience resources.
