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Global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER) protect cells against a variety of helix-distorting DNA lesions. In C. elegans, GG-NER primarily acts in proliferative germ cells and embryos, while TC-NER acts in post-mitotic somatic cells to maintain transcription. We leverage this difference to distinguish whether proteins function in GG-NER and/or TC-NER by straightforward UV survival assays. Here, we detail a protocol for these assays, using GG-NER factor xpc-1 and TC-NER factor csb-1 as examples. For complete details on the use and execution of this protocol, please refer to Sabatella et al. (2021).
Pubmed ID: 34151304
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WormBook is a comprehensive, open-access collection of original, peer-reviewed chapters covering topics related to the biology of Caenorhabditis elegans and other nematodes. Wormbook also contains WormMethods, a collection of protocols for nematode researchers, and the Worm Breeder''s Gazette, an informal, non-refereed, biannual newsletter for the interchange of ideas and information related to C. elegans and other nematodes. WormBook is the online text companion to WormBase, the C. elegans model organism database. WormBook contains original reviews on all aspects of C. elegans biology and up-to-date descriptions of technical procedures used to study this animal. WormBook Sections: *Genetics and genomics *Molecular biology *Biochemistry *Cell biology *Developmental control *Post-embryonic development *Sex determination *The germ line *Signal transduction *Neurobiology and behavior *Evolution and ecology *Disease models and drug discovery
View all literature mentionsCenter that acquires, maintains, and distributes genetic stocks and information about stocks of the small free-living nematode Caenorhabditis elegans for use by investigators initiating or continuing research on this genetic model organism. A searchable strain database, general information about C. elegans, and links to key Web sites of use to scientists, including WormBase, WormAtlas, and WormBook are available.
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