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 PMID:33537419  

The complete mitochondrial genome of Muscina pascuorum (Diptera: Muscidae).

Jingjing Huang | Zixiang Ni | Hua Wang | Li Zhang | Jie Yan | Shengbin Bai
Mitochondrial DNA. Part B, Resources | 2021

Muscina pascuorum (Diptera: Muscidae) represents an important hygiene pest. The complete mitochondrial genome (mitogenome) of M. pascuorum was first sequenced and annotated in this study. The full length of mitogenome was 14, 940 bp, consisting of 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA), 22 transfer RNA (tRNA), and one AT-rich region. The nucleotide content of these flies was 40.0% A, 13.2% C, 9.1% G, and 37.6% T. This study illustrates that the arrangement of the genes was identical to classical metazoans. Besides, the phylogenetic analyses indicated that the branch of M. pascuorum was clustered separately from the common three Muscina spp in the tree. This genome provides an essential reference for understanding the phylogenetic relationships of Muscidae.

Pubmed ID: 33537419

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NCBI (tool)

RRID:SCR_006472

A portal to biomedical and genomic information. NCBI creates public databases, conducts research in computational biology, develops software tools for analyzing genome data, and disseminates biomedical information for the better understanding of molecular processes affecting human health and disease.

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SOAPdenovo (tool)

RRID:SCR_010752

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on February 24,2023. Software tool for de novo assembly of human genomes with massively parallel short read sequencing.Short-read assembly method that can build de novo draft assembly for human sized genomes.Software package for assembling short oligonucleotide into contigs and scaffolds.

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MITObim (tool)

RRID:SCR_015056

Method for mitochondrial baiting and iterative mapping. MITObim assembles novel mitochondrial genomes of non-model organisms directly from total genomic DNA derived NGS reads.

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