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Radial glial progenitor cells (RGPs) are the major neural progenitor cells that generate neurons and glia in the developing mammalian cerebral cortex1-4. In RGPs, the centrosome is positioned away from the nucleus at the apical surface of the ventricular zone of the cerebral cortex5-8. However, the molecular basis and precise function of this distinctive subcellular organization of the centrosome are largely unknown. Here we show in mice that anchoring of the centrosome to the apical membrane controls the mechanical properties of cortical RGPs, and consequently their mitotic behaviour and the size and formation of the cortex. The mother centriole in RGPs develops distal appendages that anchor it to the apical membrane. Selective removal of centrosomal protein 83 (CEP83) eliminates these distal appendages and disrupts the anchorage of the centrosome to the apical membrane, resulting in the disorganization of microtubules and stretching and stiffening of the apical membrane. The elimination of CEP83 also activates the mechanically sensitive yes-associated protein (YAP) and promotes the excessive proliferation of RGPs, together with a subsequent overproduction of intermediate progenitor cells, which leads to the formation of an enlarged cortex with abnormal folding. Simultaneous elimination of YAP suppresses the cortical enlargement and folding that is induced by the removal of CEP83. Together, these results indicate a previously unknown role of the centrosome in regulating the mechanical features of neural progenitor cells and the size and configuration of the mammalian cerebral cortex.
Pubmed ID: 32238932
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An image processing program running under Windows suitable for such tasks as tensor calculation, color mapping, fiber tracking, and 3D visualization. Most of operations can be done with only a few clicks. This tool evolved from DTI Studio. Tools in the program can be grouped in the following way: * Image Viewer * Diffusion Tensor Calculations * Fiber Tracking and Editing * 3D Visualization * Image File Management * Region of Interesting (ROI) Drawing and Statistics * Image Registration
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentions3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.
View all literature mentionsImaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsMus musculus with name Crl:CD1(ICR) from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsThis polyclonal targets Oligodendrocute transcription factor 2
View all literature mentionsThis polyclonal targets HOPX antibody produced in rabbit
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis monoclonal targets Neurofilament Marker
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets CCDC41 antibody produced in rabbit
View all literature mentionsThis monoclonal targets Tenascin C antibody [EPR4219]
View all literature mentionsThis monoclonal targets α-Tubulin
View all literature mentionsThis unknown targets Chicken IgY (IgG) (H+L)
View all literature mentionsThis polyclonal targets SOX2
View all literature mentionsThis monoclonal targets ZO-1
View all literature mentionsThis monoclonal targets EOMES
View all literature mentionsThis monoclonal targets Vimentin (phospho S55) antibody [4A4]
View all literature mentionsThis unknown targets Human PTPRZ1
View all literature mentionsThis unknown targets Goat IgG (H+L)
View all literature mentionsThis monoclonal targets SATB2 antibody [EPNCIR130A]
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal targets BLBP - Neuronal Marker
View all literature mentionsThis monoclonal targets YAP (63.7)
View all literature mentionsThis polyclonal targets CEP164
View all literature mentionsThis polyclonal targets PARD3 antibody produced in rabbit
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets S-100 alpha/beta chain (B32.1)
View all literature mentionsThis polyclonal targets Pericentrin
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets Nestin (intermediate filament protein)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Catenin, beta
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis polyclonal targets Mouse Pax6
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Pericentrin
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets BrdU
View all literature mentionsThis monoclonal targets Ctip2
View all literature mentionsThis polyclonal targets Microtubule-Associated Protein 2 (MAP2)
View all literature mentionsThis polyclonal targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets Histone H3 (phospho S10) antibody
View all literature mentionsThis monoclonal targets N Cadherin antibody [5D5]
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