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 PMID:31193484  

Sequence variation data of the mitochondrial DNA D-loop region of the captive Malayan Gaur (Bos gaurus hubbacki).

Badrul Munir Md-Zain | Aqilah Abdul-Aziz | Nor Rahman Aifat | Nur Syafika Mohd-Yusof | Nadiatur Akmar Zulkifli | Jeffrine Rovie Ryan Japning | Norsyamimi Rosli | Salmah Yaakop
Data in brief | 2019

This article contains data of the sequence variation in the mitochondrial DNA D-loop region of the Malayan gaur (Bos gaurus hubbacki), locally known as the seladang, from two captive centers. Thirty fecal samples of Malayan gaur were collected from Jenderak Selatan Wildlife Conservation Center (Pahang) and the Sungkai Wildlife Reserve (Perak) for DNA extraction and amplification with polymerase chain reactions. DNA sequences were then analyzed using neighbor joining (NJ) and maximum parsimony (MP) methods. Based on the 652 base pairs obtained, we found seven variable characters with a value of 1%. The genetic distance between the two captive centers was 0.001. Haplotype analyses detected only four haplotypes between these two captive centers. Both NJ and MP trees demonstrate that all individuals in the Jenderak and Sungkai captive centers are in the same clade. Genetic variation of the Malayan gaur in these centers is considered low, possibly because individuals share the same common parent. This sequence variation data are of paramount importance for designing a proper breeding and management program of the Malayan gaur in the future.

Pubmed ID: 31193484

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This is a list of tools and resources that we have found mentioned in this publication.


BioEdit (tool)

RRID:SCR_007361

Software tool as biological sequence alignment editor written for Windows 95/98/NT/2000/XP/7 and sequence analysis program. Provides sequence manipulation and analysis options and links to external analysis programs to view and manipulate sequences with simple point and click operations.

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Mega BLAST (tool)

RRID:SCR_011920

Software that uses the greedy algorithm for nucleotide sequence alignment search.

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PAUP (tool)

RRID:SCR_014931

Software which creates phylogenetic trees from molecular, morphological and/or behavioral data through high speed computer analysis.

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ClustalW (tool)

RRID:SCR_017277

Web sevice of ClustalW provided by DNA data bank of Japan.

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