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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00049949
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:33027250, PMID:37782660
Synonyms: ijmSi3 I; unc-119(ed3) III; ltIs37 IV.
Notes: Generated from papers flagged positive during the last month for data type afp_otherstrain and contains genotype and gene data.|"ijmSi3 [mex-5p::cls-2(re-encoded)::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] I. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. The coding sequence of GFP-tagged cls-2 in the transgene was re-coded using silent mutations to render it insensitive to RNAi-depletion of endogenous cls-2 expression. mCherry expression marks histones. Not known if unc-119(ed3) is still carried in the background of this strain. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Schlientz A and Bowerman B. PLoS Genet. 2020 Oct 7;16(10):e1008751. doi: 10.1371/journal.pgen.1008751."|"Made_by: Aleesa Schlientz"|"Supplementary_genotype ijmSi3 [pJD342/pJD330; ChrI_5mex-5_cls-2reenc::GFP_3tbb-2; cb-unc-119(+)]I; unc-119(ed3) III?; itIs37[pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV"
Proper citation: RRID:WB-STRAIN:WBStrain00049949 Copy
http://www.wormbase.org/db/get?name=WBStrain00050174
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:34003969
Synonyms: vsSi32 unc-119(ed3) III.
Notes: Generated from papers flagged positive during the last month for data type afp_otherstrain and contains genotype and gene data.|"vsSi32 [goa-1::GFP + unc-119(+)] III. GOA-1::GFP translational fusion driven by 5kb of goa-1 promoter. Rescues unc-119 locomotion defect. vsSi32 is inserted into the left arm of chromosome III between sequences 5-tttactgcatactgaacaacaggggaaaagggg-3 and 5-tagaattagctgtaagacggcgtctaggttttgca-3. Reference: Kumar S, et al. G3 (Bethesda). 2021 Aug 7;11(8):jkab167. doi: 10.1093/g3journal/jkab167. PMID: 34003969"
Proper citation: RRID:WB-STRAIN:WBStrain00050174 Copy
http://www.wormbase.org/db/get?name=WBStrain00050636
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: antIs30 II; unc-119(ed9) III.
Notes: antIs30 [attP-f + Cbr-unc-119(+) + glh-2p::phiC31 + rol-6(partial) + myo-2p::GFP + attP-r] II. antIs30 was inserted into ttTi5605 on LG II using MosSCI. GFP expression in pharynx is very weak (as it is single copy) and is easiest to see during the L1-L3 stages. This strain contains a phiC31 docking site and can be used for precise single-copy integration of transgenes via recombination mediated cassette exchange. The docking site contains inverted phiC31-attP sites flanking phiC31 integrase expressed from the glh-2 germline promoter. Integration constructs need to have inverted phiC31-attB sites that flank the intended sequence to be inserted. Reference: Yang FJ, et al. phiC31 integrase for recombination mediated single copy insertion and genome manipulation in C. elegans. Genetics 2021.|"Made_by: Fang-Jung Yang"
Proper citation: RRID:WB-STRAIN:WBStrain00050636 Copy
http://www.wormbase.org/db/get?name=WBStrain00050674
Source Database: WormBase (WB)
Affected Genes: WBGene00001067(dpy-5)|WBGene00002225(klp-15)|WBGene00002226(klp-16)|WBGene00004027(pie-1)|WBGene00006843(unc-119)|WBGene00008107(aspm-1)
Genomic Alteration: WBGene00001067(dpy-5), WBGene00002225(klp-15), WBGene00002226(klp-16), WBGene00004027(pie-1), WBGene00006843(unc-119), WBGene00008107(aspm-1)
Availability: unknown
Synonyms: klp-15(ok1958) aspm-1(syb1260[gfp::aspm-1]) klp-16(or1952) /tmC18[dpy-5(tmIs1236)] I; ltIs37[pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV
Notes: itIs37 [pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. GFP tag inserted into endogenous aspm-1 locus. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry triple mutant homozygotes. Homozygous triple mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020|"ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. GFP tag inserted into endogenous aspm-1 locus. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry triple mutant homozygotes. Homozygous triple mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020"
Proper citation: RRID:WB-STRAIN:WBStrain00050674 Copy
http://www.wormbase.org/db/get?name=WBStrain00050678
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: gwIs39 gwIs34 unc-119(ed3) III.
Notes: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. gwIs34 [myo-3::mCherry + 256xlacO + unc-119(+)] III. Superficially wild-type. Expresses GFP-LacI throughout development from early embryogenesis, forming a small spot at the lacO array. Worms have red muscle (from L1 stage) and green intestine (from late L4 stage). Reference: Meister P, et al. Genes Dev. 2010 Apr 15;24(8):766-82. PMID: 20395364
Proper citation: RRID:WB-STRAIN:WBStrain00050678 Copy
http://www.wormbase.org/db/get?name=WBStrain00050639
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:39652010
Synonyms: muIs252 II; unc-119(ed3) III.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Somatic expression of wrmScarlet1-10 (under the control of the eft-3 promoter and unc-54 3'UTR). Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628
Proper citation: RRID:WB-STRAIN:WBStrain00050639 Copy
http://www.wormbase.org/db/get?name=WBStrain00050698
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: unc-119(ed3) III; goeIs257.
Notes: goeIs257 [nas- 38p::d1mGFP::nas-38 3'UTR + unc-119(+)]. Destabilized GFP expressed from the nas-38 promoter; especially visible in hypodermis and excretory system. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791|"Made_by: Florentin Masurat"
Proper citation: RRID:WB-STRAIN:WBStrain00050698 Copy
http://www.wormbase.org/db/get?name=WBStrain00050761
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)|WBGene00009163(drsh-1)|WBGene00011908(pash-1)
Genomic Alteration: WBGene00006843(unc-119), WBGene00009163(drsh-1), WBGene00011908(pash-1)
Availability: unknown
Synonyms: drsh-1(luc82[myc::AID::3XFLAG::4xGGSG::drsh-1::4xGGSG::3xFLAG::AID::myc]) pash-1(luc71[pash-1::2xGGSG::3xFLAG::AID::myc]) I; ieSi57 II; unc-119(ed3) III; ieSi38 IV; lucIs20; lucIs24.
Notes: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. lucIs20 [mir-35p::mirtron-35 + myo-2::mCherry]. lucIs24 [mir-52p::mirtron-51 + elt-2::dsRed + myo-2::mCherry]. Endogenous drsh-1 tagged at both N- and C-termini with the auxin-inducible-degron (AID) peptide. Endogenous pash-1 tagged with the AID peptide at the C-terminus. Strain expresses modified Arabidopsis thaliana TIR1 tagged with mRuby in soma and germline. In addition, strain expresses mirtron-versions of mir-35 and mir-51, which are processed independently of Drosha and Pasha. miRNA biogenesis can be stringently inhibited via simultaneous removal of Drosha and Pasha, causing absence of all canonical miRNAs and embryonic lethality upon Auxin treatment. Reference: Dexheimer, PJ, et al. Curr Biol. 2020. in press.|"Made_by: Philipp Dexheimer"
Proper citation: RRID:WB-STRAIN:WBStrain00050761 Copy
http://www.wormbase.org/db/get?name=WBStrain00050748
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: lucSi100 II; unc-119(ed3) III.
Notes: lucSi100 [hsp16.41::vhhGFP4::zif-1::SL2::mCherry::his-11::tbb-2 3'UTR] II. Superficially wild-type morphology. Single-copy insertion of a GFP-nanobody::zif-1 fusion transgene under a heat-shock promoter for timely controlled degradation of GFP-tagged proteins (Wang et al. (2017). A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Development 144, 2694-2701.)
Proper citation: RRID:WB-STRAIN:WBStrain00050748 Copy
http://www.wormbase.org/db/get?name=WBStrain00050747
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)|WBGene00011908(pash-1)
Genomic Alteration: WBGene00006843(unc-119), WBGene00011908(pash-1)
Availability: unknown
Synonyms: pash-1(luc71[pash-1::2xGGSG::3xFLAG::AID::myc]) I; ieSi57 II; unc-119(ed3) III; ieSi38 IV.
Notes: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Endogenous pash-1 tagged with the auxin-inducible-degron (AID) peptide at the C-terminus. Strain expresses modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and soma. Animals are superficially wild-type; addition of auxin induces embryonic lethality and larval arrest phenotypes. Reference: Dexheimer, PJ, et al. Curr Biol. 2020. in press.|"Made_by: Philipp Dexheimer"
Proper citation: RRID:WB-STRAIN:WBStrain00050747 Copy
http://www.wormbase.org/db/get?name=WBStrain00051037
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)|WBGene00017088(akir-1)
Genomic Alteration: WBGene00006843(unc-119), WBGene00017088(akir-1)
Availability: unknown
Synonyms: akir-1(iow88[GFP11::akir-1]) I; iowSi8 II; unc-119(ed3) III.
Notes: akir-1(iow88[GFP11::akir-1]) I. iowSi8[pie-1p::GFP1-10::him-3 3UTR + Cbr-unc119(+)] II. CRISPR/Cas9 insertion of GFP11 tag into endogenous akir-1 locus in background strain SSM471. GFP::AKIR-1 fluorescence only observed in the germline. If germline silencing occurs, transgene expression can be recovered by growing worms at 25C for 2 generations. Reference: Hefel A & Smolikove S. G3. 2019 Jun 5;9(6):1933-1943. PMID: 30992318.|"Made_by: Adam Hefel"
Proper citation: RRID:WB-STRAIN:WBStrain00051037 Copy
http://www.wormbase.org/db/get?name=WBStrain00040117
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; wwIs18.
Alternate IDs: WB-STRAIN:VL412, CGC_VL412
Notes: Made_by: N Martinez/M Ow|"wwIs18 [mir-79p::GFP + unc-119(+)]. Wild type."
Proper citation: RRID:WB-STRAIN:WBStrain00040117 Copy
http://www.wormbase.org/db/get?name=WBStrain00040115
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; wwEx31.
Alternate IDs: WB-STRAIN:VL397, CGC_VL397
Notes: Made_by: N Martinez/M Ow|"wwEx31 [mir-268p::GFP + unc-119(+)]. Maintain by picking WT."
Proper citation: RRID:WB-STRAIN:WBStrain00040115 Copy
http://www.wormbase.org/db/get?name=WBStrain00040121
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; wwEx32.
Alternate IDs: WB-STRAIN:VL431, CGC_VL431
Notes: Made_by: N Martinez/M Ow|"wwEx32 [mir-61-250p::GFP + unc-119(+)]. Maintain by picking WT."
Proper citation: RRID:WB-STRAIN:WBStrain00040121 Copy
http://www.wormbase.org/db/get?name=WBStrain00040124
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; wwIs9.
Alternate IDs: WB-STRAIN:VL442, CGC_VL442
Notes: Made_by: N Martinez/M Ow|"wwIs9 [mir-392p::GFP + unc-119(+)]. Wild type."
Proper citation: RRID:WB-STRAIN:WBStrain00040124 Copy
http://www.wormbase.org/db/get?name=WBStrain00040123
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; wwIs11.
Alternate IDs: WB-STRAIN:VL440, CGC_VL440
Notes: Made_by: N Martinez/M Ow|"wwIs11 [mir-47p::GFP + unc-119(+)]. Wild type."
Proper citation: RRID:WB-STRAIN:WBStrain00040123 Copy
http://www.wormbase.org/db/get?name=WBStrain00040125
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; wwIs17.
Alternate IDs: WB-STRAIN:VL445, CGC_VL445
Notes: Made_by: N Martinez/M Ow|"wwIs17 [mir-76p::GFP + unc-119(+)]. Wild type."
Proper citation: RRID:WB-STRAIN:WBStrain00040125 Copy
http://www.wormbase.org/db/get?name=WBStrain00040106
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; wwEx28.
Alternate IDs: WB-STRAIN:VL205, CGC_VL205
Notes: Made_by: N Martinez/M Ow|"wwEx28 [mir-72p::GFP + unc-119(+)]. Maintain by picking non-Unc."
Proper citation: RRID:WB-STRAIN:WBStrain00040106 Copy
http://www.wormbase.org/db/get?name=WBStrain00040105
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; wwIs3.
Alternate IDs: WB-STRAIN:VL200, CGC_VL200
Notes: Made_by: N Martinez/M Ow|"wwIs3 [mir-236::GFP + unc-119(+)]. Wild-type."
Proper citation: RRID:WB-STRAIN:WBStrain00040105 Copy
http://www.wormbase.org/db/get?name=WBStrain00040103
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; wwEx20.
Alternate IDs: WB-STRAIN:VL187, CGC_VL187
Notes: Made_by: N Martinez/M Ow|"wwEx20 [mir-245p::GFP + unc-119(+)]. Maintain by picking non-Unc."
Proper citation: RRID:WB-STRAIN:WBStrain00040103 Copy
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