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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00054754
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: F13E6.1(utx75[F13E6.1::mNG::3xFlag]) X.
Notes: C-terminal tag of F13E6.1 via CRISPR/Cas9 knock-in of mNeonGreen at F13E6.1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd 5 AAATCGTGCTCTCCCAAGCA 3; rev 5 CTTGTCACCTGACGGGATGT 3. Left flank: 5' CCAGTTGCGGAGGAGGCGAAGCCAATCTCT 3' (1 silent mutation); Right flank: 5' TAAattcattcatttcacataccaatatgt 3'; sgRNA: atgaatTTAAGAGATTGGCT; Cas9/sgRNA plasmid: pGLOW26; mNG^SEC^3xFlag plasmid: pGLOW104; SEC insertion allele strain: GLW94.|"Made_by: Gonzalo Botello-Lins (Glow Worms '20)"
Proper citation: RRID:WB-STRAIN:WBStrain00054754 Copy
http://www.wormbase.org/db/get?name=WBStrain00054755
Source Database: WormBase (WB)
Affected Genes: WBGene00004323(rde-1)
Genomic Alteration: WBGene00004323(rde-1)
Availability: unknown
Source References: EMPTY
Synonyms: rde-1(ar660) V.
Notes: Made_by: Justin Shaffer|"rde-1(ar660)[rde-1(flexon)]. 9th intron of rde-1 replaced with a flexon - an artificial exon flanked by artificial introns that each contain a lox2272 site (a flexon). Severely reduces rde-1 function, function can be restored upon excision of the flexon by Cre recombinase. Reference: Shaffer JM & Greenwald I. Proc Natl Acad Sci U S A. 2022 Jan 18;119(3):e2117451119. doi: 10.1073/pnas.2117451119. PMID: 35027456."|"rde-1(ar660)[rde-1(lox2272-flexon-lox2272)]. 9th intron of rde-1 replaced with a flexon - an artificial exon flanked by artificial introns that each contain a lox2272 site (a flexon). Severely reduces rde-1 function, function can be restored upon excision of the flexon by Cre recombinase. Reference: Shaffer JM & Greenwald I. Proc Natl Acad Sci U S A. 2022 Jan 18;119(3):e2117451119. doi: 10.1073/pnas.2117451119. PMID: 35027456."
Proper citation: RRID:WB-STRAIN:WBStrain00054755 Copy
http://www.wormbase.org/db/get?name=WBStrain00054740
Source Database: WormBase (WB)
Affected Genes: WBGene00002123(inx-1)
Genomic Alteration: WBGene00002123(inx-1)
Availability: unknown
Source References: EMPTY
Synonyms: inx-1(tm3524) X.
Notes: Derived by out-crossing parental strain FX18538 five times.|"Made_by: Savannah Sojka"
Proper citation: RRID:WB-STRAIN:WBStrain00054740 Copy
http://www.wormbase.org/db/get?name=WBStrain00054747
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: H34C03.2(utx55[mNG::3xFlag::H34C03.2]) IV.
Notes: Made_by: Christine Collins, Vinay Pillai, Wesley Yeung|"N-terminal tag of H34C03.2 via CRISPR/Cas9 knock-in of mNeonGreen at H34C03.2 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gggattgcctacactcaaatatacgtaacg 3'; Right flank: 5' ATGCCCACGCCGGAAGTGTTCCCATGGATG 3 (4 silent mutations); gRNA: cgtaacgATGCCAACTCCCG; Cas9/sgRNA plasmid: pGLOW9; mNG^SEC^3xFlag plasmid: pGLOW106; SEC insertion allele strain: GLW72."
Proper citation: RRID:WB-STRAIN:WBStrain00054747 Copy
http://www.wormbase.org/db/get?name=WBStrain00054742
Source Database: WormBase (WB)
Affected Genes: WBGene00006747(unc-7)
Genomic Alteration: WBGene00006747(unc-7)
Availability: unknown
Source References: EMPTY
Synonyms: unc-7(e5) X.
Notes: Derived by out-crossing parental strain CB5.|"Made_by: Savannah Sojka"
Proper citation: RRID:WB-STRAIN:WBStrain00054742 Copy
http://www.wormbase.org/db/get?name=WBStrain00054745
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: gjIs140.
Notes: gjIs140 [gpa-4::GFP + dpy-20(+)]. Reporter construct includes 2.5 kb upstream and the first exon of gpa-4 fused in frame with GFP. Reference: Burghoorn et al. Proc. Natl. Acad. Sci. USA 2007 Apr; 104(17):7157-62.|"Made_by: Gert Jansen lab"
Proper citation: RRID:WB-STRAIN:WBStrain00054745 Copy
http://www.wormbase.org/db/get?name=WBStrain00054744
Source Database: WormBase (WB)
Affected Genes: WBGene00001079(dpy-20)
Genomic Alteration: WBGene00001079(dpy-20)
Availability: unknown
Source References: EMPTY
Synonyms: dpy-20(e1282) IV; pkIs1201.
Notes: Made_by: Plasterk lab|"pkIs1201 [gpa-3::GFP + dpy-20(+)]."
Proper citation: RRID:WB-STRAIN:WBStrain00054744 Copy
http://www.wormbase.org/db/get?name=WBStrain00054738
Source Database: WormBase (WB)
Affected Genes: WBGene00001072(dpy-10)|WBGene00006787(unc-52)|WBGene00006994(zyg-9)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00006787(unc-52), WBGene00006994(zyg-9)
Availability: unknown
Source References: EMPTY
Synonyms: zyg-9(or1985)/mnC1[dpy-10(e128) unc-52(e444) umnIs32] II.
Notes: umnIs32 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. or1985 is a CRISPR/Cas9 engineered deletion of zyg-9 removing the entire open reading frame. Heterozygotes are wild-type and GFP+ and segregate WT GFP+ (hets), or1948 homozygotes (GFP-, lay 100% dead embryos) and paralysed DpyUnc GFP+ (mnC1 homozygotes). Maintain by picking WT GFP+. Reference: Harvey AM, et al. PLoS Genet. 2023 Jan 6;19(1):e1010363. doi: 10.1371/journal.pgen.1010363. PMID: 36608115
Proper citation: RRID:WB-STRAIN:WBStrain00054738 Copy
http://www.wormbase.org/db/get?name=WBStrain00054739
Source Database: WormBase (WB)
Affected Genes: WBGene00002123(inx-1)
Genomic Alteration: WBGene00002123(inx-1)
Availability: unknown
Source References: EMPTY
Synonyms: inx-1(tm6662) X.
Notes: Derived by out-crossing parental strain FX18537 five times.|"Made_by: Savannah Sojka"
Proper citation: RRID:WB-STRAIN:WBStrain00054739 Copy
http://www.wormbase.org/db/get?name=WBStrain00054793
Source Database: WormBase (WB)
Affected Genes: WBGene00006604(tra-1)
Genomic Alteration: WBGene00006604(tra-1)
Availability: unknown
Source References: EMPTY
Synonyms: tra-1(q106) III/eT1[qIs60](III;V).
Notes: qIs60 [pes-10::GFP + gut specific promoter::GFP + myo-2::GFP]. Heterozygotes are wild-type GFP+ and segregate wild-type GFP+ heterozygotes, non-GFP males, and dead eggs (eT1 homozygotes). Pick wild-type GFP+ and check for proper segregation of progeny to maintain. Reference: Schedl T, et al. Genetics. 1989 Dec;123(4):755-69. doi: 10.1093/genetics/123.4.755. PMID: 2612895.
Proper citation: RRID:WB-STRAIN:WBStrain00054793 Copy
http://www.wormbase.org/db/get?name=WBStrain00054795
Source Database: WormBase (WB)
Affected Genes: WBGene00000254(bli-4)|WBGene00001609(glp-1)
Genomic Alteration: WBGene00000254(bli-4), WBGene00001609(glp-1)
Availability: unknown
Source References: EMPTY
Synonyms: glp-1(q172) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Notes: Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q172 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Crittenden SL, et al. Development. 1994 Oct;120(10):2901-11. doi: 10.1242/dev.120.10.2901. PMID: 7607080.
Proper citation: RRID:WB-STRAIN:WBStrain00054795 Copy
http://www.wormbase.org/db/get?name=WBStrain00054792
Source Database: WormBase (WB)
Affected Genes: WBGene00014165(puf-12)
Genomic Alteration: WBGene00014165(puf-12)
Availability: unknown
Source References: EMPTY
Synonyms: puf-12(q810) II.
Notes: Made_by: Aaron Kerschner|"Superficially wild-type."
Proper citation: RRID:WB-STRAIN:WBStrain00054792 Copy
http://www.wormbase.org/db/get?name=WBStrain00054836
Source Database: WormBase (WB)
Affected Genes: WBGene00021711(wago-11)
Genomic Alteration: WBGene00021711(wago-11)
Availability: unknown
Source References: EMPTY
Synonyms: wago-11(tor129[GFP::3xFLAG::wago-11]) II.
Notes: GFP and 3xFLAG tags inserted into endgonenous wago-11 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00054836 Copy
http://www.wormbase.org/db/get?name=WBStrain00054798
Source Database: WormBase (WB)
Affected Genes: WBGene00008924(F17E5.2)
Genomic Alteration: WBGene00008924(F17E5.2)
Availability: unknown
Source References: EMPTY
Synonyms: F17E5.2(q864) X.
Notes: 350 bp deletion in F17E5.2|"Made_by: Erika Sorensen"
Proper citation: RRID:WB-STRAIN:WBStrain00054798 Copy
http://www.wormbase.org/db/get?name=WBStrain00054831
Source Database: WormBase (WB)
Affected Genes: WBGene00004093(ppw-1)
Genomic Alteration: WBGene00004093(ppw-1)
Availability: unknown
Source References: EMPTY
Synonyms: ppw-1(tor119[GFP::3xFLAG::ppw-1c]) I.
Notes: GFP and 3xFLAG tags inserted into endgonenous ppw-1; specifically tags c-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00054831 Copy
http://www.wormbase.org/db/get?name=WBStrain00054797
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: q833 V/nT1[qIs51](IV; V).
Notes: hets are green pharynx WT and segregate green pharynx WT, fertile non-green pharynx and dead eggs (nT1 homozygotes). q833 is a 472 bp deletion into the intergenic region between mir-61/ mir-250 and F55A11.4.|"Made_by: Erika Sorenson"
Proper citation: RRID:WB-STRAIN:WBStrain00054797 Copy
http://www.wormbase.org/db/get?name=WBStrain00054830
Source Database: WormBase (WB)
Affected Genes: WBGene00010263(wago-4)
Genomic Alteration: WBGene00010263(wago-4)
Availability: unknown
Source References: EMPTY
Synonyms: wago-4(tor117[GFP::3xFLAG::wago-4]) II.
Notes: GFP and 3xFLAG tags inserted into endgonenous wago-4 locus. Reference: Lev I, et al. Curr Biol. 2019 Sep 9;29(17):2880-2891.e4. PMID: 31378614
Proper citation: RRID:WB-STRAIN:WBStrain00054830 Copy
http://www.wormbase.org/db/get?name=WBStrain00054799
Source Database: WormBase (WB)
Affected Genes: WBGene00003083(lst-1)
Genomic Alteration: WBGene00003083(lst-1)
Availability: unknown
Source References: EMPTY
Synonyms: lst-1(q867) I.
Notes: CRIPSR-engineered mutation of the lst-1M71 longform start codon (ATG to AT, deletion of G). Reference: Haupt KA, et al. Development. 2019 Oct 17;146(20):dev181644. doi: 10.1242/dev.181644. PMID: 31515205|"Made_by: Alex Murphy"
Proper citation: RRID:WB-STRAIN:WBStrain00054799 Copy
http://www.wormbase.org/db/get?name=WBStrain00054783
Source Database: WormBase (WB)
Affected Genes: WBGene00010677(gtbp-1)
Genomic Alteration: WBGene00010677(gtbp-1)
Availability: unknown
Source References: PMID:37254647
Synonyms: gtbp-1(ax4561[gtbp-1p::IBB domain::mNeonGreen::gtbp-1 3'utr]) IV.
Notes: Made_by: Alex Paix|"The Importin Beta Binding domain (IBB)::mNeonGreen reporter replaces gtbp-1 in the endogenous gtbp-1 locus. Sterile at 25C; maintain at 20C. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans"
Proper citation: RRID:WB-STRAIN:WBStrain00054783 Copy
http://www.wormbase.org/db/get?name=WBStrain00054785
Source Database: WormBase (WB)
Affected Genes: WBGene00001569(meg-3)|WBGene00003994(pgl-3)
Genomic Alteration: WBGene00001569(meg-3), WBGene00003994(pgl-3)
Availability: unknown
Source References: EMPTY
Synonyms: pgl-3(ax4516[pgl-3(delta448-693)::3xFLAG]) V; meg-3(ax3054[meg-3::meGFP]) X.
Notes: 3xFlag tag inserted at truncated C-terminus of endogenous pgl-3 locus. Inserted into parental strain JH3503 meg-3(ax3054[meg-3::meGFP]). Reference: Ouyang JPT, et al. Nature Cell Biology 2022 (24)11291140. DOI: 10.1038/s41556-022-00940-w.|"Made_by: Brennan Danlasky"
Proper citation: RRID:WB-STRAIN:WBStrain00054785 Copy
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