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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type D (wild type D; wild type D, II)
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01496 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (twi1[HAn,3’neo3]; TWI1::HA-TWi1-neo3, pm-r, VII)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: N-terminal HA tag on TWI1 with 3’ flanking neo3
Source References: PMID:12297043
Notes: CU428 was transformed with HA_TWI1-neo3 and endogenous TWI1 loci were completely replaced. See Mochizuki et al (2002) Cell 110,689-699. Chaker and Allis labs have some strains.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02069 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Source References: PMID:9343391, PMID:16908532
Notes: Somatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01374 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HTAZ ; MTT1/mtt1[5’neo2,∆::HTAZ(K4R,K7R,K10R,K13R K16R,,K21R)]; pm-r, H2A.Z RRRRRRneo regulated by MTT1 promoter, VII)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Macronucleus: inserted HTAZ with K4R, K7R, K10R, K13R K16R, , K21R Into MTT1 locus and neo2 in 5’flanking of MTT1
Source References: PMID:12665578, PMID:11430834
Notes: Cell has wt H2A.Z in its locus, and a mutated H2A.Z RRRRRR copy in MTT1 locus, neo2 cassette is inserted at 5’ of MTT, complete replacement ?. Somatic transformation.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02463 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (tcd2[∆?::neo4]/TCD2[5’bsr1]; Mix of pm-r and bs-r, II)
Affected Genes: HPL7 (TTHERM_00551070)
Genomic Alteration: Macronucleus: TCD2 was knocked out with neo4, then the wt TCD2 gene with a bsr1cassette upstream was somatically reintroduced into the cell
Notes: Using Complete somatic Neo4 knockout Tcd2 strains. Tcd2 gene was reintroduced with blasticidin cassette (H4-BSR-B) upstream.
H3K27 methylation was recovered.
Bsr/Bsr, Tcd2/Tcd2, Neo4/Neo4 This is what Wei wrote, not sure if the replacement was complete or if both resistance are still present.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02506 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E441D, E443D, E445D,E446D,E447D ]; pm-r, mp-?, ?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E441D, E443D, E445D, E446D, E447D or neo replacing coding of some ATU1 genes
Source References: PMID:9037049, PMID:10831613
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pATU5D derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E441D, E443D, E445D,E446D,E447D. Slow growth.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02229 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TTHERM_00137600[∆::NEO2] (TTHERM_00137600[∆::NEO2]; timing and location of basal body assembly, ?)
Affected Genes: Sas6b
Genomic Alteration: Micronucleus: TTHERM_00118700[∆::NEO2]
Source References: PMID:19158390
Notes: From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03323 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MPR1-1/mpr1-1; TWI7/twi7[∆?::neo3] (mpr1-1; pm-r, ?)
Affected Genes: TWI7 (TTHERM_00600450)
Genomic Alteration: Micronucleus: Partial coding of TWI7 replaced by neo3
Notes: heterozygote TWI7 KO heterokaryon Partial TWI7 coding sequences were replaced by Neo3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02870 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: oad1/oad1; chx1-1/chx1-1? (oad1; cy-s)
Source References: PMID:20189389, PMID:8401477
Notes: Submitted by Jacek Gaertig. Originally created by D. Pennock.
Proper citation: RRID:TSC_SD01784 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: homozygous HMG C KO with BSR (mp-r)
Affected Genes: HMGB (TTHERM_00660180)
Notes: Obtained thru 1st round exclusion of ∆B∆C gl KO. Genotype confirmed by cross with 427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01388 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2] (hta1[∆::neo2], htax[∆::neo2]/htax [S1P,,K5R,K8R,K10R, K12R,K17R, S122A, k123R, S124A, K125R, K126R, T127A, S129A]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: HTAX and HTA1 KO with neo Macronucleus: Rescue with mutated HTAX with S1P, , K5R, K8R, K10R, K12R, K17R, S122A, k123R, S124A, K125R, K126R, T127A, S129A
Source References: PMID:17242195
Notes: HTAX S1P+5R+4A+3R mutation somatic rescue strain of HTA1, HTA2 double knockout
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02199 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-EF4; Basal body disorganization and stability defects (TS)., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-EF3
Source References: PMID:21562224
Notes: EF-hand mutant allele rescue of cen1∆ - D145A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03288 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (RDR1?/rdr1[neo3;HA,FLAG]; pm-r
, ?)
Affected Genes: RDR1 (TTHERM_00853150)
Genomic Alteration: Macronucleus: HA-FLAG tagged RDR1 with neo3 in flank
Notes: Shot plasmid pRDP1::TAG-neo3 #110 into conjugating CU428 X B2086 but only got somatic strains, slow growth No other information, don’t know if complete replacement
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02513 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4]
(drh1[∆?::neo4]/drh1[∆?::neo4]; pm-r, ?)
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: neo4 at 5’end of coding, some coding remains Macronucleus: neo4 at 5’ end of coding, some coding remains
Notes: Homozygous homokaryon KO of DRH1 with neo4 cassette. Mates with DRH1g∆∆ C10-11F
Gene is Dicer Related Helicase, sequence is fromTGD 69.m00174. Mating cells stop at mic selection after MeiosisII Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
Needs Cd for pm resistance.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02599 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pm-r)
Affected Genes: HHO1 (TTHERM_00823720) + HHP1 (TTHERM_00705240)
Notes: Complete somatic knockout of H1 andHHP1. See Hui Huang thesis for details. Max paromomycin 100 mg/ml. Retransform ∆HHP1 cells with ∆H1 construct.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02637 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[∆::neo2]/htaz[∆::neo2] (htax[K123R]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[K130R]/htaz[∆;;neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 knockout of HTAX, HTA1, HTAZ Macronucleus: mutant HTAX and HTAZ rescue of mac KO
Source References: PMID:19822522
Notes: HTAX/HTAZ double mutant rescue; Rescue of h∆∆HTA∆HTZ1-F1B1 and B1C5 with Dryl's.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02403 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (V)
Source References: PMID:25973525
Notes: Collected by Paul Doerder on 8/26/2008 from the pond designated SG69-4 in Guys Mills, PA (lat. 41 38.023, long. -79 53.514, elevation 1660 ft). Old pond sign says pond #19.
Geographical area: Western Pennsylvania
Collection number: 892
Has a micronucleus.
Cech's self-splicing intron is present. Cytochrome oxidase I haplotype = WPA1
Proper citation: RRID:TSC_SD01555 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[5’neo2,GFPc]/hht3[5’neo2,GFPc] (?)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Micronucleus: GFP tagged HHT3
Source References: PMID:16908532
Notes: star side of H3.3+GFP germline replacement heterokaryon.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01952 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: act1[208::neo3]/act1[208::neo3] (pm-s , mp-?, ?)
Affected Genes: ACT1 (TTHERM_00190950)
Genomic Alteration: Micronucleus: ACT1 with neo3 inserted at Sfu1 site
Source References: PMID:16524910
Notes: ACT1∆ KO homozygous in mic, heterokaryon
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01798 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E447A]; pm-r, mp-?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E447A or neo replacing coding of some ATU1 genes
Source References: PMID:10831613, PMID:9037049
Notes: It is a progeny of conjugants of AAK02.7 X AAKO5.5 transformed with pA447 derived from pTUB100E3-PvuII (linearized) where the ATU1 gene is marked with Hae III and Pvu II sites and E447A.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01831 Copy
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