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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00062660
Source Database: WormBase (WB)
Affected Genes: WBGene00003071(lrp-1)
Genomic Alteration: WBGene00003071(lrp-1)
Availability: unknown
Source References: EMPTY
Synonyms: lrp-1(wrd320[lrp-1::mNG::3xFLAG]) I.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted internally in the last exon of the endogenous lrp-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062660 Copy
http://www.wormbase.org/db/get?name=WBStrain00062665
Source Database: WormBase (WB)
Affected Genes: WBGene00000602(col-13)
Genomic Alteration: WBGene00000602(col-13)
Availability: unknown
Source References: EMPTY
Synonyms: col-13(wrd344[col-13::mNG::3xFLAG]) V.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-13 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062665 Copy
http://www.wormbase.org/db/get?name=WBStrain00062662
Source Database: WormBase (WB)
Affected Genes: WBGene00000928(dbd-2)
Genomic Alteration: WBGene00000928(dbd-2)
Availability: unknown
Source References: EMPTY
Synonyms: dao-2(wrd328[dao-2::mNG::3xFLAG]) II.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous dao-2 locus by CRISPR using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062662 Copy
http://www.wormbase.org/db/get?name=WBStrain00062663
Source Database: WormBase (WB)
Affected Genes: WBGene00001074(dpy-13)
Genomic Alteration: WBGene00001074(dpy-13)
Availability: unknown
Source References: EMPTY
Synonyms: dpy-13(syb3318(wrd337[dpy-13::30x linker::mScarlet(dpi)::10x linker]) IV.
Notes: 30x linker::mScarlet(dpi)::10x linker tag inserted into the C-terminus of the endogenous dpy-13 locus by CRISPR using a Cas9 RNP. Derived by modification of syb3318[dpy-13::mNG] in parental strain PHX3318, replacing mNeonGreen with the mScarlet and linkers.
Proper citation: RRID:WB-STRAIN:WBStrain00062663 Copy
http://www.wormbase.org/db/get?name=WBStrain00062668
Source Database: WormBase (WB)
Affected Genes: WBGene00010677(gtbp-1)
Genomic Alteration: WBGene00010677(gtbp-1)
Availability: unknown
Source References: EMPTY
Synonyms: gtbp-1(ax5000[gtbp-1::tagRFP]) IV.
Notes: Made_by: Sean Lee|"tagRFP tag inserted into endogenous gtbp-1 locus. Reference: Lee, CYS, et al. Elife. 020 Jan 24:9:e52896. doi: 10.7554/eLife.52896. PMID: 31975687."
Proper citation: RRID:WB-STRAIN:WBStrain00062668 Copy
http://www.wormbase.org/db/get?name=WBStrain00062700
Source Database: WormBase (WB)
Affected Genes: WBGene00001072(dpy-10)|WBGene00001401(fbf-1)|WBGene00001402(fbf-2)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00001401(fbf-1), WBGene00001402(fbf-2)
Availability: unknown
Source References: EMPTY
Synonyms: fbf-1(ok91) fbf-2(q1272[*q1023])/mIn1 [mIs14 dpy-10(e128)] II.
Notes: Made_by: Brian Carrick|"Pick wild-type GFP+ to maintain. 3xFlag tag inserted into endogenous fbf-2 locus with engineered (H453A H454A E457A Y479A) substitutions. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (Sterile). Pick WT dim GFP and check for correct segregation of progeny to maintain."
Proper citation: RRID:WB-STRAIN:WBStrain00062700 Copy
http://www.wormbase.org/db/get?name=WBStrain00062659
Source Database: WormBase (WB)
Affected Genes: WBGene00000739(col-166)
Genomic Alteration: WBGene00000739(col-166)
Availability: unknown
Source References: EMPTY
Synonyms: col-166(wrd319[col-166::mNG::3xFLAG]) X.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted internally to produce a translational fusion after amino acid 120 in the endogenous col-166 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062659 Copy
http://www.wormbase.org/db/get?name=WBStrain00062650
Source Database: WormBase (WB)
Affected Genes: WBGene00009384(piit-1)
Genomic Alteration: WBGene00009384(piit-1)
Availability: unknown
Source References: EMPTY
Synonyms: piit-1(wrd302[piit-1::mNG::3xFLAG]) V.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous piit-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062650 Copy
http://www.wormbase.org/db/get?name=WBStrain00062653
Source Database: WormBase (WB)
Affected Genes: WBGene00000699(col-125)
Genomic Alteration: WBGene00000699(col-125)
Availability: unknown
Source References: EMPTY
Synonyms: col-125(wrd300 wrd312[col-125::mScarlet::2xOLLAS]) IV.
Notes: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous col-125 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd300.
Proper citation: RRID:WB-STRAIN:WBStrain00062653 Copy
http://www.wormbase.org/db/get?name=WBStrain00062652
Source Database: WormBase (WB)
Affected Genes: WBGene00013352(lon-8)
Genomic Alteration: WBGene00013352(lon-8)
Availability: unknown
Source References: EMPTY
Synonyms: lon-8(wrd305[lon-8::mNG::3xFLAG]) V.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous lon-8 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062652 Copy
http://www.wormbase.org/db/get?name=WBStrain00062657
Source Database: WormBase (WB)
Affected Genes: WBGene00013352(lon-8)
Genomic Alteration: WBGene00013352(lon-8)
Availability: unknown
Source References: EMPTY
Synonyms: lon-8(wrd305 wrd317[lon-8::mScarlet::2xOLLAS]) V.
Notes: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous lon-8 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd305.
Proper citation: RRID:WB-STRAIN:WBStrain00062657 Copy
http://www.wormbase.org/db/get?name=WBStrain00062658
Source Database: WormBase (WB)
Affected Genes: WBGene00011522(srap-1)
Genomic Alteration: WBGene00011522(srap-1)
Availability: unknown
Source References: EMPTY
Synonyms: srap-1(wrd318[srap-1::mNG::3xFLAG]) II.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted in the first exon after the signal sequenceof the endogenous srap-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062658 Copy
http://www.wormbase.org/db/get?name=WBStrain00062656
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: ctsa-1.1(wrd303 wrd315[ctsa-1.1::mScarlet::2xOLLAS]) II.
Notes: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous ctsa-1.1 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd303.
Proper citation: RRID:WB-STRAIN:WBStrain00062656 Copy
http://www.wormbase.org/db/get?name=WBStrain00062648
Source Database: WormBase (WB)
Affected Genes: WBGene00000699(col-125)
Genomic Alteration: WBGene00000699(col-125)
Availability: unknown
Source References: EMPTY
Synonyms: col-125(wrd300[col-125::mNG::3xFLAG]) IV.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-125 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062648 Copy
http://www.wormbase.org/db/get?name=WBStrain00062640
Source Database: WormBase (WB)
Affected Genes: WBGene00000601(col-12)
Genomic Alteration: WBGene00000601(col-12)
Availability: unknown
Source References: EMPTY
Synonyms: col-12(wrd279[col-12::mNG::3xFLAG]) V.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-12 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062640 Copy
http://www.wormbase.org/db/get?name=WBStrain00062641
Source Database: WormBase (WB)
Affected Genes: WBGene00000618(col-41)
Genomic Alteration: WBGene00000618(col-41)
Availability: unknown
Source References: EMPTY
Synonyms: col-41(wrd280[col-41::mNG::3xFLAG]) X.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted 6 bp before the stop codon in the endogenous col-41 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062641 Copy
http://www.wormbase.org/db/get?name=WBStrain00062647
Source Database: WormBase (WB)
Affected Genes: WBGene00010723(cpg-7)
Genomic Alteration: WBGene00010723(cpg-7)
Availability: unknown
Source References: EMPTY
Synonyms: cpg-7(wrd287[cpg-7:::mNG::3xFLAG]) II.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous cpg-7 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062647 Copy
http://www.wormbase.org/db/get?name=WBStrain00062639
Source Database: WormBase (WB)
Affected Genes: WBGene00021095(mlt-8)
Genomic Alteration: WBGene00021095(mlt-8)
Availability: unknown
Source References: EMPTY
Synonyms: mlt-8(wrd278[mlt-8::mNG::3xFLAG]) II.
Notes: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous mlt-8 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.
Proper citation: RRID:WB-STRAIN:WBStrain00062639 Copy
http://www.wormbase.org/db/get?name=WBStrain00062738
Source Database: WormBase (WB)
Affected Genes: WBGene00003622(nhr-23)
Genomic Alteration: WBGene00003622(nhr-23)
Availability: unknown
Source References: EMPTY
Synonyms: nhr-23(kry61[nhr-23::AID*::TEV::3xFLAG]) I; ieSi57 II.
Notes: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Strain for somatic depletion of NHR-23::AID*::TEV::3xFLAG using the auxin-inducible degron system. Derived by crossing parental strains KRY87 and CA1200. Reference: Zhang L, et al. Development. 2015 Dec 15;142(24):4374-84. doi: 10.1242/dev.129635. PMID: 26552885.
Proper citation: RRID:WB-STRAIN:WBStrain00062738 Copy
http://www.wormbase.org/db/get?name=WBStrain00062694
Source Database: WormBase (WB)
Affected Genes: WBGene00000254(bli-4)|WBGene00001595(gld-1)
Genomic Alteration: WBGene00000254(bli-4), WBGene00001595(gld-1)
Availability: unknown
Source References: EMPTY
Synonyms: gld-1(q1243) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Notes: Pick GFP+ to maintain. CRIPSR-engineered modification of gld-1 FBEa and FBEa* in gld-1 3'UTR. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1243 homozygotes (sterility/reduced fertility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Increase in distal GLD-1 protein levels and decrease in proximal GLD-1 protein levels. Qiu et al., in preparation.
Proper citation: RRID:WB-STRAIN:WBStrain00062694 Copy
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