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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[3’neo2, (T35E, S43E, S45E, T47E, T54E, T37K,A44K, T46K,V49K,V53K), GFPc]; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Change Threonine 35, 47, 54 and serine 43, 45 to glutamic acid (charge patch) and T37K, A44K, T46K, V49K, V53K and GFP tag
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Notes: Complete somatic tranformant of CU428 cells with pHHO5KE5 /neo construct. Phosphorylation sites 1- 5 of H1 changed to glutamic acid and 5 other sites changed to lysines,c-terminal GFP neo 2 cassette in 3’ flanking region for selection. See paper
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02478 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ift122[∆::neo3]/ift122[∆::neo3] (WT; pm-?, mp-?, ?)
Affected Genes: IFT122 (TTHERM_00694540)
Genomic Alteration: Micronucleus: Neo3 into coding of IFT122A
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Notes: This is the non-star side of the IFT122 KO heterokaryon.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01787 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: WT (giw1-?[∆::neo3]; ∆p115, pm-r (neo3), II)
Affected Genes: p115, GIW1 (TTHERM_01276320)
Genomic Alteration: Macronucleus: KO of GIW1 with neo3
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Notes: Somatic KO of p115, in B2086
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01823 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ift122[∆::neo3]/ift122[∆::neo3]; ift172[∆::neo3]/ift172[∆::neo3] (WT; pm-?, mp-?, ?)
Affected Genes: IFT122 (TTHERM_00694540), IFT172 (TTHERM_00089240)
Genomic Alteration: Micronucleus: Neo3 into coding of IFT122A and of IFT172
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Notes: IFT122 and IFT172 double KO heterokaryon
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01789 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (MTT1/mtt1[5’neo2, ∆, 2HAn(::HTA.Y 1-258)]; pm-r, ?)
Affected Genes: HTAY (TTHERM_01079200)
Genomic Alteration: Macronucleus: Region of HTAY gene encoding AA 1 to 258 with two HA tags at n-terminus was inserted into the MTT1 gene.
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Notes: Somatic transfomation of CU428xB2086 with pXS88. (MTT-neo2-2HA-HTA.Y n258) into MTT1 locus, Incomplete replacement
n258 means AA 1-258 of HTA.Y are present
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02884 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
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Proper citation: RRID:TSC_SD01156 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: lkn1-1/lkn1-1 (lkn1-1, V)
Affected Genes: LKN1 (low kinety number 1)
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Notes: A reduced number of ciliary rows combined with a reduced fidelity in transmission of pre-existing row numbers, probably owing to interruptions and irregularities in the rows.
The phenotype is unconditional and penetrance is 100%. Cell mutiplication is unaffected.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01434 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (cnjB[∆?::neo3]; pm-r, VI)
Affected Genes: CnjB (TTHERM_01091290)
Genomic Alteration: Macronucleus: Neo3 replaces part of CnjB coding
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Notes: somatic CnjB KO. Partial CnjB coding sequences were replaced by Neo3. Background: CU427.
Grow in 0.5 to 1.0 ug/ml CdCl2 and 100 ug/ml paromomycin.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02767 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆HHT2/HHF2;.∆HHT1;∆HHF1 homozygous heterkaryon (pm-r)
Affected Genes: HHT1 (TTHERM_00570560),HHT2 (TTHERM_00189180), HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170)
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Notes: These are intermediate germlineKO heterokaryons not tested for somatic rescue. Final KO strains are #4 and#7. This is the star side.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02006 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1; Nulli 3 (CHX1; mpr1-1; cy-s, mp-r, IV)
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Proper citation: RRID:TSC_SD00108 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 2L (CHX1; pmr1-1; cy-s, pm-r, II)
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Notes: Deletion on left arm of chromosome 2.
Proper citation: RRID:TSC_SD00909 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; MPR1[C3]/MPR1[C3]; pmr1-1/pmr1-1; MAT3/MAT3 (CHX1[C3]; cy-s, VII)
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Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-s, pm-r, mat3
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01282 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (V)
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Notes: Collected by Paul Doerder on 7/24/2009 from the pond designated Gregg Lake in Antrim, NH (lat. 43 02.605, long. -71 59.383, elevation 1063 ft). Boat access near beach, great veg.
Geographical area: New England
Collection number: 1175
Has a micronucleus.
Proper citation: RRID:TSC_SD01560 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1[C3]-1/pmr1[C3]-1 (pmr1-1[C3]; pm-r, ?)
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Proper citation: RRID:TSC_SD00195 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1[∆,::neo1]/btu1[∆,::neo1]; btu2[∆,::bsr1]/btu2[∆,::bsr1] (assorted to pm-s so BTU1
assorted to bsr-s so BTU2; mp-r, VII)
Affected Genes: BTU1 (TTHERM_00348510) + BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: BTU1 coding replaced with neo1 cassette, BTU2 is blasticidin KO. Macronucleus: assorted to WT or lost resistance cassettes
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Notes: Obtained from Jacek Gaertig 3/8/2000. Germline KO BTU1 neo1 cassette; BTU2 is blasticidin KO. Mates with DB2Apm-1.-2,-3 for heterokaryon rescue.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02014 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S10A,S28A]; pm-r, ?)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo knockout of HHT1, HHT2, HHF1 Macronucleus: KO of HHT1, HHF1, and for HHT2 have mutations S10A and S28A
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Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S10AS28A #6-later after seq.transformant found a wrong nucleotide~25 bases upsteam of ATG, transformant seems normal.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02375 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: bp3[3’neo3,∆::GFP]/rbp3[3’neo3,∆::GFP];mpr1-1/mpr1-1; chx1-1/chx1-1 (mp-?, cy-?, ?)
Affected Genes: RPB3 (TTHERM_00193849)
Genomic Alteration: Micronucleus: GFP replaces RPB3 coding, neo3 in 3’ flanking region. homozygous
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Notes: Homozygous heterokaryon of ∆RPB3 (=cnjc). B2086 X Cu428 background, can mate with he∆ccg+-1-1
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02654 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (cy-r, II)
Affected Genes: PPO1 (TTHERM_00865290)
Genomic Alteration: Macronucleus: Ppo1p with n-terminal V5 tag, cells very sick, not used in paper
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Notes: B2086 cells Transformed with V5-PP50 with the cycloheximide resistance cassette H4-rpL29-B upstream. Partial replacement . Cells are very sick, give yellow-green auto fluorescence with many vacuoles.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02539 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ubc2[∆::neo2]/ubc2[∆::neo2] (VI)
Affected Genes: UBC2 (TTHERM_00550720)
Genomic Alteration: Micronucleus: Neo2 KO of UBC2
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Notes: UBC2 KO homozygous heterokaryon. Mates with ∆UBC2gl 1B2.8 and 1B2.10
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02538 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (Circular permutation of HHO1 charge patch, impossible to define in correct nomenclature, see paper.; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
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Notes: Somatic transformation of CU428 with HHO1 construct W-N
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02543 Copy
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