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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-EF4; Basal body disorganization and stability defects (TS)., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-EF3
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Notes: EF-hand mutant allele rescue of cen1∆ - D145A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03288 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (RDR1?/rdr1[neo3;HA,FLAG]; pm-r
, ?)
Affected Genes: RDR1 (TTHERM_00853150)
Genomic Alteration: Macronucleus: HA-FLAG tagged RDR1 with neo3 in flank
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Notes: Shot plasmid pRDP1::TAG-neo3 #110 into conjugating CU428 X B2086 but only got somatic strains, slow growth No other information, don’t know if complete replacement
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02513 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4]
(drh1[∆?::neo4]/drh1[∆?::neo4]; pm-r, ?)
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: neo4 at 5’end of coding, some coding remains Macronucleus: neo4 at 5’ end of coding, some coding remains
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Notes: Homozygous homokaryon KO of DRH1 with neo4 cassette. Mates with DRH1g∆∆ C10-11F
Gene is Dicer Related Helicase, sequence is fromTGD 69.m00174. Mating cells stop at mic selection after MeiosisII Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
Needs Cd for pm resistance.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02599 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pm-r)
Affected Genes: HHO1 (TTHERM_00823720) + HHP1 (TTHERM_00705240)
Genomic Alteration:
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Notes: Complete somatic knockout of H1 andHHP1. See Hui Huang thesis for details. Max paromomycin 100 mg/ml. Retransform ∆HHP1 cells with ∆H1 construct.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02637 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[∆::neo2]/htaz[∆::neo2] (htax[K123R]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[K130R]/htaz[∆;;neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 knockout of HTAX, HTA1, HTAZ Macronucleus: mutant HTAX and HTAZ rescue of mac KO
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Notes: HTAX/HTAZ double mutant rescue; Rescue of h∆∆HTA∆HTZ1-F1B1 and B1C5 with Dryl's.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02403 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (V)
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Notes: Collected by Paul Doerder on 8/26/2008 from the pond designated SG69-4 in Guys Mills, PA (lat. 41 38.023, long. -79 53.514, elevation 1660 ft). Old pond sign says pond #19.
Geographical area: Western Pennsylvania
Collection number: 892
Has a micronucleus.
Cech's self-splicing intron is present. Cytochrome oxidase I haplotype = WPA1
Proper citation: RRID:TSC_SD01555 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[5’neo2,GFPc]/hht3[5’neo2,GFPc] (?)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Micronucleus: GFP tagged HHT3
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Notes: star side of H3.3+GFP germline replacement heterokaryon.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01952 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: act1[208::neo3]/act1[208::neo3] (pm-s , mp-?, ?)
Affected Genes: ACT1 (TTHERM_00190950)
Genomic Alteration: Micronucleus: ACT1 with neo3 inserted at Sfu1 site
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Notes: ACT1∆ KO homozygous in mic, heterokaryon
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01798 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E447A]; pm-r, mp-?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E447A or neo replacing coding of some ATU1 genes
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Notes: It is a progeny of conjugants of AAK02.7 X AAKO5.5 transformed with pA447 derived from pTUB100E3-PvuII (linearized) where the ATU1 gene is marked with Hae III and Pvu II sites and E447A.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01831 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[5’neo3,HAn]/twi11[5’neo3,HAn] (twi11[5’neo3,HAn]; pm-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
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Notes: Homozygous HA-tagged TWI 11 homokaryon.HA coding sequence was inserted just after the start codon of TWI11. neo3 cassette was inserted upstream of TWI11 coding.
mates with HA-TWI11 F2-1, F2-8.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02804 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BLT1/ blt1-1[∆,neo3]; MPR1-?/ MPR1-? (BLT1; mpr1-1; mp-r, ?)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Micronucleus: Neo3 replaces BLT coding
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Notes: BLT germline heterokaryon, heterozygous KO in mic, pm sensitive (120 ug/ml). Cd induced Neo cassette (1.2 ug/ml)--MTT promoter, Neo coding, BTU2 terminator. Construct pBLTMKO,
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02253 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: rbp3[3’neo3,∆::GFP]/rbp3[3’neo3,∆::GFP];mpr1-1/mpr1-1; chx1-1/chx1-1 (mp-?, cy-?, ?)
Affected Genes: RPB3 (TTHERM_00193849)
Genomic Alteration: Micronucleus: GFP replaces RPB3 coding, neo3 in 3’ flanking region. homozygous
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Notes: Homozygous heterokaryon of ∆RPB3 (=cnjc). B2086 X Cu428 background, can mate with he∆ccg+-4-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02653 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (pm-r)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration:
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Notes: Same as B2086-XS49 (15) but diff. cell line.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02136 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E445D]; pm-r, mp-?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E445D, some have neo1 replacing coding
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Notes: It is a progeny of conjugants of AAK02.7 X AAKO5.5 transformed with pD445 derived from pTUB100E3-PvuII (linearized) where the ATU1 gene is marked with Hae III and Pvu II sites and E445D. Partial replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02772 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janA-1/janA-1; disA-1/disA-1 (janA-1; disA-1; janA, disA, IV)
Affected Genes: JANA (janusA), DISA (disorganizedA)
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Notes: Additive expression:the janus phenotype is superimposed upon a disorganized cortical landscape. It is uncertain whether or not eja1-1 is present in this stock.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01569 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2; CHX1/CHX1; mpr1?/mpr1? (mp-r, VII)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: Neo2 cassette knockout of HTAX coding region
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Notes: HTA1 Germline knockout heterokaryon. Transformed CU428 X B2086 with pQR10B (removes all coding, replaces with neo cassette). It can mate with HTA1 G1 14B.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02812 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (hta1[ S134A]; hta2[∆::neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTA1 and HTA2 genes Macronucleus: mutated HTA1
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Notes: It is somatic rescue of strain of HTA1,HTA2 double knockout by HTA1 S134A mutation.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02384 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (chx1-1; cy-r, ory-r, tax-s, VII)
Affected Genes:
Genomic Alteration: Micronucleus: btu1-1::btu1-1M350K/btu1-1::btu1-1M350K (homozygous) Macronucleus: btu1-1::btu1-1M350K (homozygous)
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Proper citation: RRID:TSC_SD00481 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU-HA-TAP]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
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Notes: Rescue of GTU05XGTU06 knockout heterokaryons with pTTMG-HA TAP. GTU1 with HA+TAP tag into MTT1 locus. MTT1 promoter, grow with 0.2ug/ml CdCl in 1xSPP.
5’MTT1-GTU-HA-TAP-MTT1 3’
TAP tag from Eric Phzicky is:
GTU-HA-6XHis-3Cprotease cleavage-S.Aureus protein A. Constuct pTTMG-HA used to rescue GTU5 xGTU06 KO heterokaryons.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02146 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-2/hpo1-2; bcd1-1/bcd1-1 (hpo1-2; bcd1-1; hpo1, bcd1, IV)
Affected Genes: HPO1 (hypoangular1), BCD1 (broadened cortical domains1)
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Notes: hpo1-2 strongly antagonizes the broadening of CVP domains brought about by bcd1-1; the resulting phenotype is a compromise between the broadening of the CVP domains brought about by bcd1 and the narrowing of these domains generated by hpo1
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01577 Copy
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