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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00037354
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001072(dpy-10)|WBGene00017300(rpc-2)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00017300(rpc-2)
Availability: available
References:
Synonyms: +/mT1 II; F09F7.3(ok3162)/mT1 [dpy-10(e128)] III.
Alternate IDs: WB-STRAIN:VC2479, CGC_VC2479
Notes: F09F7.3. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok3162 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: ATACCCAACAGCAGGCACTC. External right primer: TTCGACAATTCCGTCATCAA. Internal left primer: TGTTACCTCAAAAGTCAAGGCT. Internal right primer: CGATTGGTTAGAGAACGGGA. Internal WT amplicon: 1204 bp. Deletion size: 794 bp. Deletion left flank: ACGTTTGGAGCTCGCTGGATCATTGCTTTC. Deletion right flank: TGGTGAAAGCCGTCAGAGATTTGAGAAGGT. Insertion Sequence: AT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037354 Copy
http://www.wormbase.org/db/get?name=WBStrain00037359
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001554(gcy-34)
Genomic Alteration: WBGene00001554(gcy-34)
Availability: available
References:
Synonyms: gcy-34(ok2953) V.
Alternate IDs: WB-STRAIN:VC2486, CGC_VC2486
Notes: M04G12.3. External left primer: GATTCTGATTTGCTTTCGGG. External right primer: ATCGACCTCAAATTCCGTTG. Internal left primer: CCGCACCTCTTTCATGATCT. Internal right primer: CATTTGCAATTTTATCAATGCTG. Internal WT amplicon: 1184 bp. Deletion size: 668 bp. Deletion left flank: ACTTTGTAAACACCACGAAGAACCACAATT. Deletion right flank: TTTTCACTTGAAGTACAAAAACAGCATTGA.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037359 Copy
http://www.wormbase.org/db/get?name=WBStrain00037363
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00016670(ilys-3)
Genomic Alteration: WBGene00016670(ilys-3)
Availability: available
References:
Synonyms: ilys-3(ok3222) IV.
Alternate IDs: WB-STRAIN:VC2496, CGC_VC2496
Notes: C45G7.3. External left primer: ATGCCAAAATCAAATGCACA. External right primer: CCACCTAAACACTTCCGTCC. Internal left primer: CTCCACTTCCTGTTTGCCAT. Internal right primer: TATGGGGTTTCCTGCAGATT. Internal WT amplicon: 1156 bp. Deletion size: 855 bp. Deletion left flank: TGAACTGTATCTTTGGTAGAATGGGTAGTA. Deletion right flank: CAGGGAGCATGCGAAGTGATGGCTCGTAAC.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037363 Copy
http://www.wormbase.org/db/get?name=WBStrain00037329
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00002040(hum-7)
Genomic Alteration: WBGene00002040(hum-7)
Availability: available
References:
Synonyms: hum-7(ok3054) I.
Alternate IDs: WB-STRAIN:VC2436, CGC_VC2436
Notes: F56A6.2. External left primer: CCTAAGAAACAGTGGCCGAG. External right primer: TTGACGAGGTTGATGAGCAC. Internal left primer: TTGACGTCACTTCTCTTCCAGA. Internal right primer: CAGGACCTCTCTTGCTAGGC. Internal WT amplicon: 1199 bp. Deletion size: 653 bp. Deletion left flank: CGAACTTTGTAAGATTCACAGCATGAACCT. Deletion right flank: ATAATTTCTACTTATATTCAGTCATATAAA. Insertion Sequence: AATAT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037329 Copy
http://www.wormbase.org/db/get?name=WBStrain00037324
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00007007(mak-2)
Genomic Alteration: WBGene00007007(mak-2)
Availability: available
References:
Synonyms: mak-2(gk1110) IV.
Alternate IDs: WB-STRAIN:VC2426, CGC_VC2426
Notes: C44C8.6. The gk1110 deletion allele in this strain was identified and isolated using PCR, and its breakpoints were determined by capillary sequencing of PCR products. Its presence was confirmed by the Vancouver Gene Knockout Lab by CGH (comparative genome hybridization). External left primer: ACTCTGTGCCACCAAAAACC. External right primer: CATATCCGTCCATTGTTCCC. Internal left primer: TGTCCTGCTTCAGTTTCCCT. Internal right primer: CATTGGTTGTCCGTGTTGAG. Internal WT amplicon: 1976 bp. Deletion size: 1024 bp. Deletion left flank: GAATTTTTAAATCAAAACTATTTGTTCCAA. Deletion right flank: GCGTATGACTAAGTCTATGCCTAAGCCTAA. Insertion Sequence: T.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037324 Copy
http://www.wormbase.org/db/get?name=WBStrain00037327
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004811(skr-5)
Genomic Alteration: WBGene00004811(skr-5)
Availability: available
References:
Synonyms: skr-5(ok3068) V.
Alternate IDs: WB-STRAIN:VC2434, CGC_VC2434
Notes: F47H4.10. External left primer: ATTTGTTGGGCGGTTATGAA. External right primer: CCGTCTCCACATCCATCTCT. Internal left primer: TTGATTTAAGATGGTTGTGGAAGA. Internal right primer: GCCTCCACCAACTGAAGAAG. Internal WT amplicon: 1342 bp. Deletion size: 813 bp. Deletion left flank: CTTCTGATGATGTAGAGTTCATTGTAGGTT. Deletion right flank: GAATAGGGAGTTAAAATAGGAAATAAAATA.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037327 Copy
http://www.wormbase.org/db/get?name=WBStrain00037328
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00010873(trpa-2)
Genomic Alteration: WBGene00010873(trpa-2)
Availability: available
References:
Synonyms: M05B5.6(ok3189) I.
Alternate IDs: WB-STRAIN:VC2435, CGC_VC2435
Notes: M05B5.6. External left primer: CAAAGGCGATGTCATTTGTG. External right primer: CTCGTCCAACTCACACCAGA. Internal left primer: AAAGCTGAACCAGGATTTTATGA. Internal right primer: TCCGGGAAATAAAGAGAAGGA. Internal WT amplicon: 1127 bp. Deletion size: 478 bp. Deletion left flank: GGCCATTTCAGATGAGGTAATATTTTACTT. Deletion right flank: GGATATTTGGACATTTGAGTTCCCCAAAAT.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037328 Copy
http://www.wormbase.org/db/get?name=WBStrain00037325
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00008205(sams-1)
Genomic Alteration: WBGene00008205(sams-1)
Availability: available
References:
Synonyms: sams-1(ok2946) X.
Alternate IDs: WB-STRAIN:VC2428, CGC_VC2428
Notes: C49F5.1. External left primer: AGGACTTGCGAGAGTACGGA. External right primer: CTTGAGAGCTTTTGGCTGCT. Internal left primer: AGTGAATCTGTGTCCGAGGG. Internal right primer: GGGAACTCAGAGTGACCGAA. Internal WT amplicon: 1248 bp. Deletion size: 707 bp. Deletion left flank: TAGCTTGTTTCAATGTTTTTGTTCAGGATT. Deletion right flank: TCGGAACTTCCCCACTCACCGACGAAGAGC.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037325 Copy
http://www.wormbase.org/db/get?name=WBStrain00037331
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003938(pax-2)
Genomic Alteration: WBGene00003938(pax-2)
Availability: available
References:
Synonyms: pax-2(ok3078) IV.
Alternate IDs: WB-STRAIN:VC2438, CGC_VC2438
Notes: K06B9.5. External left primer: AGGCTAATGAAACGTGCCAA. External right primer: AATTTTCGAGTCGTTGGTCG. Internal left primer: ACAGAGAAATGGCGAGTTGC. Internal right primer: CACGTGGGTAATGTGGTACG. Internal WT amplicon: 1183 bp. Deletion size: 527 bp. Deletion left flank: AACTAAAAAGTGTACTTTATTGAGATTATA. Deletion right flank: GTGTTCTCCGACAAGTCCAGTAGGATAGAC. Insertion Sequence: GTGTACTTTATTGAGATTATA.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037331 Copy
http://www.wormbase.org/db/get?name=WBStrain00037334
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000405(cdk-1)|WBGene00001072(dpy-10)
Genomic Alteration: WBGene00000405(cdk-1), WBGene00001072(dpy-10)
Availability: available
References:
Synonyms: +/mT1 II; cdk-1(ok1881)/mT1 [dpy-10(e128)] III.
Alternate IDs: WB-STRAIN:VC2446, CGC_VC2446
Notes: Mutagen:UV/TMP|"T05G5.3. Homozygous sterile deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok1881 homozygotes (sterile Unc). Pick WT and check for correct segregation of progeny to maintain. External left primer: CACTAAGCAATGGTCTCGCA. External right primer: CGACAACAATGGAAACATCG. Internal left primer: CGCTTACGCCTTTTCTATCG. Internal right primer: ACCATTCTCTCGTGAATCCG. Internal WT amplicon: 2136 bp. Deletion size: 1140 bp. Deletion left flank: AATCGGCGAAGGAACATACGGAGTCGTCTA. Deletion right flank: TCGTCTTCCGTGTATTGTTTAACTATCACC."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037334 Copy
http://www.wormbase.org/db/get?name=WBStrain00037332
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003527(nas-8)
Genomic Alteration: WBGene00003527(nas-8)
Availability: available
References:
Synonyms: nas-8(ok3137) IV.
Alternate IDs: WB-STRAIN:VC2439, CGC_VC2439
Notes: C34D4.9. External left primer: ACCATTATCCCACAAATGCC. External right primer: TGGAAGCTTCACATCACTGC. Internal left primer: TACACGAAAATGGCCAAATG. Internal right primer: ATTGGTACATCAGATTCATTTTTAA. Internal WT amplicon: 1132 bp. Deletion size: 422 bp. Deletion left flank: CTGCGTTCGATTTGTTCCTAGAACCGCTGT. Deletion right flank: TTGTATTTGCACAGTATAGATTTGCAAATC.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037332 Copy
http://www.wormbase.org/db/get?name=WBStrain00037333
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00017803(F26A1.4)
Genomic Alteration: WBGene00017803(F26A1.4)
Availability: available
References:
Synonyms: F26A1.4(gk1167) III.
Alternate IDs: WB-STRAIN:VC2441, CGC_VC2441
Notes: F26A1.4. External left primer: TTTAGGTCTGGCACTACCCG. External right primer: AAAACATTGACACACCTGCG. Internal left primer: AAAGCGGCAGCAGTTAAGAA. Internal right primer: CTACCGGTACTGCCATTCGT. Internal WT amplicon: 1327 bp. Deletion size: 174 bp. Deletion left flank: TTATGTTTATGTTTCAGTTCTGACACGCCA. Deletion right flank: TTAAAATATTTCAGATTGAATGTGGAGATG.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037333 Copy
http://www.wormbase.org/db/get?name=WBStrain00037338
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001542(gcy-17)
Genomic Alteration: WBGene00001542(gcy-17)
Availability: available
References:
Synonyms: gcy-17(gk1155) I.
Alternate IDs: WB-STRAIN:VC2450, CGC_VC2450
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"W03F11.2. External left primer: TCTAGGTCAAAAGCGGAGGA. External right primer: CTTTAACACGGTGAAGGGGA. Internal left primer: GGAAATGGAGCATCGAGGTA. Internal right primer: CATATGCAAGATGTTTGCCG. Internal WT amplicon: 1241 bp. Deletion size: 655 bp. Deletion left flank: GCATTGAGCTTCTGCTAATGACATCGGCCA. Deletion right flank: TAAATTTTGAGAGTAAAGTTCTTACATTTC."
Proper citation: RRID:WB-STRAIN:WBStrain00037338 Copy
http://www.wormbase.org/db/get?name=WBStrain00037336
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019205(kcc-2)
Genomic Alteration: WBGene00019205(kcc-2)
Availability: available
References:
Synonyms: kcc-2(ok3074) IV/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2448, CGC_VC2448
Notes: H16O14.1. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3074 homozygotes (sterile with no eggs, often with vulval blip). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GGACGGCCAGATTCAGTAAA. External right primer: CGTAATGGCGGTTTTTGATT. Internal left primer: TTTGGTAACTTCTGGCCGTC. Internal right primer: GGGGCTTGTTTGAAAGAACA. Internal WT amplicon: 1227 bp. Deletion size: 582 bp. Deletion left flank: CAAACTAAGTATTTATTCTTTTAACATTTT. Deletion right flank: AGTAATTCTTTTTGGATGTTTCATGTCAAC. Insertion Sequence: TAAAAAGTATTTATTCTTTTAACATTCTTTTAACAC.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037336 Copy
http://www.wormbase.org/db/get?name=WBStrain00037308
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00012888(sas-6)
Genomic Alteration: WBGene00012888(sas-6)
Availability: available
References:
Synonyms: sas-6(ok2554) IV/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2399, CGC_VC2399
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y45F10D.9. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok2554 homozygotes (sterile, no eggs). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CTCATGAGAATCCCCGTTGT. External right primer: ACGGGATAAGTGGCTCACAC. Internal left primer: CGGAGGACTCCCAACTGATA. Internal right primer: TGAAAATGCGGGAAACTCTC. Internal WT amplicon: 2129 bp. Deletion size: 1435 bp. Deletion left flank: TATTGTCACGGAATGGGGTGCGCTGAAATT. Deletion right flank: CTGTTACTTTTGAAAATCGTTTGCTCCCTT."
Proper citation: RRID:WB-STRAIN:WBStrain00037308 Copy
http://www.wormbase.org/db/get?name=WBStrain00037302
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001545(gcy-20)|WBGene00005692(sru-29)
Genomic Alteration: WBGene00001545(gcy-20), WBGene00005692(sru-29)
Availability: available
References:
Synonyms: gkDf17 II; gkDf18 C50C10.2(gk3035) gcy-20(gk1184) V.
Alternate IDs: WB-STRAIN:VC2391, CGC_VC2391
Notes: C40A11.7, C40A11.8, C40A11.1, F56E10.1, Y38C9A.1, C50C10.2, F21H7.9. The allele gk1184 was identified by PCR, validated by CGH, and can be detected using the following PCR primers. External left primer: AATCACTTTCGGTGCAGCTT. External right primer: GTATGCCCCACAGTTTTGCT. Internal left primer: AGTATCGCGGCATTGTTAGC. Internal right primer: TGCTCAAGCTTGGAGAGACA. Internal WT amplicon: 2419 bp. Deletion size: 2042 bp. Deletion left flank: ACCGCAATTAATTCCAATTCTAAGGTTTAT. Deletion right flank: ACTGGCGTCTTACAGTAAATTTTGTGTGAC. The allele gkDf17 was identified by CGH but not confirmed by PCR. Left flanking probe: ATTCCGCGATGTCTCCTTAAATCTTTTGGCAGAGGTTCTCGATTATCCAT. Right flanking probe: ATTGATCGAAAGTTACGAAGACGTGGACTAGTCCCAAAATTCCTAGTGAC. Left deleted probe: GAAAATAGATTTCTACCACTGAACTGTTTTTCTTAACAAACTCATCGAAT. Right deleted probe: CTGTTGAGAACATATCTAGTATTAAGGAAGGAGGGAACTATTCCACAGGC. The allele gkDf18 was identified by CGH but not confirmed by PCR. Left flanking probe: CGAATTTTCGAGGAAGATGAAGTTTATGCGGACGTCCAAAGTGTTGAAAA. Right flanking probe: GATTTCGCTGTGATAAGCGTCGAGGAGGCAATCGAAATGTGGAGCTTCTG. Left deleted probe: CCAAAGTGTTGAAAAACGGAAAATTCAGGATTTCGACGAGCGAATTGAGG. Right deleted probe: CAATTATGCAAATCTCGTCGATATTATACAAAATGATATAGATTTCGCTG. The allele gk3035 was identified by CGH but not confirmed by PCR. Left flanking probe: TGTTTCAGTATTGCCGTCTTATTATGTATAGATTTGCTATTCCATTTCTA. Right flanking probe: CATTTTCGAGTTCAATTTTCTGTGCAAACGCTGGAATGACAATATTCATG. Left deleted probe: TTTATCGTCCCATTAGCATTGTCACTTTTCAATGTTACTACAGTAGGATT. Right deleted probe: TTGTACGAAGGAGAGGAATATGCAAAGTTGAATGCTATTATTCATCTGTC.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037302 Copy
http://www.wormbase.org/db/get?name=WBStrain00037387
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00022632(ZC581.2)
Genomic Alteration: WBGene00022632(ZC581.2)
Availability: available
References:
Synonyms: ZC581.2(gk1146) I.
Alternate IDs: WB-STRAIN:VC2530, CGC_VC2530
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZC581.2. External left primer: TGTCTCCCAACTTCTCGTCC. External right primer: CAATGAAGAATGATCGGGCT. Internal left primer: GGAAACTTTGGAGCGTTCTG. Internal right primer: TTTACGACGTGTTCCATCCA. Internal WT amplicon: 1387 bp. Deletion size: 95 bp. Deletion left flank: TTGATCAATGTGAAATTTTGCGCAGAATCA. Deletion right flank: TTCCCGGAAGATGTTGTCATCAAAATTGAG."
Proper citation: RRID:WB-STRAIN:WBStrain00037387 Copy
http://www.wormbase.org/db/get?name=WBStrain00037388
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00018569(F47F2.1)
Genomic Alteration: WBGene00018569(F47F2.1)
Availability: available
References:
Synonyms: F47F2.1(gk1140) X.
Alternate IDs: WB-STRAIN:VC2531, CGC_VC2531
Notes: F47F2.1. Identified by PCR, validated by CGH. External left primer: TGAAAGTGCTCAACATTCGG. External right primer: CAAGGGGGAGCTATACACCA. Internal left primer: AGCATCACGGTGAGTGGTTA. Internal right primer: CATACATCCGATGCGTTGAG. Internal WT amplicon: 1402 bp. Deletion size: 548 bp. Deletion left flank: AGTAATTGAGAAAGATTTTGGTTGCAATTT. Deletion right flank: TGATGGTCGGAAAGCCCCCATTCCGTGGAA. Insertion Sequence: T.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037388 Copy
http://www.wormbase.org/db/get?name=WBStrain00037305
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004170(pqn-90)
Genomic Alteration: WBGene00004170(pqn-90)
Availability: available
References:
Synonyms: pqn-90(gk1086) IV.
Alternate IDs: WB-STRAIN:VC2394, CGC_VC2394
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y73F8A.8. External left primer: ACAACCCGTGCAAGAAAAAC. External right primer: AAGTGGGACGGAACTGTTTG. Internal left primer: ACAATCGCGTCAGTAGGAGC. Internal right primer: CAGGGTTGTAGGACGTTGGT. Internal WT amplicon: 1894 bp. Deletion size: 519 bp. Deletion left flank: AACTGAAGGTTCTAGGATGACAGTCACAATTTGTGGAGCAACAACTGGAGATTGGCATG CTGATTGGCATTGCTGACATTGTGGAGCAGCTGGTTGCTGACATTGTGGAATACATTGT TGTTGGCACTGTTGAGTCTGTTGGCACGAAGTTTGACATTGTTGGCAAGCTGGTGCAGA TGGAAGTGTGCATTGTGGAGCACACTGTTGCTGGCAAACTGGAGCATACTGCTGGCAAG TATTCTGGCACTGCTGGCACTGTGGAGCTGATGGCTGTTGGCA. Deletion right flank: CACTTGGTAAGTTACTTGCTGAACTGGTTGGGCACATGAGCAGGATGTCTGGACTGGAG CAGTGTTCTGACACGAACAACTGTACTGTTGTGGTTGAGTTGCTTGTTGGCATGAACAA CTTGGTTGAACTTGTGAAGCACAACCACATGATTGACGTTTATCACGAATTGAA. Validation: No CGH probes for gk1086."|"Y73F8A.8. External left primer: ACAACCCGTGCAAGAAAAAC. External right primer: AAGTGGGACGGAACTGTTTG. Internal left primer: ACAATCGCGTCAGTAGGAGC. Internal right primer: CAGGGTTGTAGGACGTTGGT. Internal WT amplicon: 1894 bp. Deletion size: 519 bp. Deletion left flank: AACTGAAGGTTCTAGGATGACAGTCACAATTTGTGGAGCAACAACTGGAGATTGGCATGCTGATTGGCATTGCTGACATTGTGGAGCAGCTGGTTGCTGACATTGTGGAATACATTGTTGTTGGCACTGTTGAGTCTGTTGGCACGAAGTTTGACATTGTTGGCAAGCTGGTGCAGATGGAAGTGTGCATTGTGGAGCACACTGTTGCTGGCAAACTGGAGCATACTGCTGGCAAGTATTCTGGCACTGCTGGCACTGTGGAGCTGATGGCTGTTGGCA. Deletion right flank: CACTTGGTAAGTTACTTGCTGAACTGGTTGGGCACATGAGCAGGATGTCTGGACTGGAGCAGTGTTCTGACACGAACAACTGTACTGTTGTGGTTGAGTTGCTTGTTGGCATGAACAACTTGGTTGAACTTGTGAAGCACAACCACATGATTGACGTTTATCACGAATTGAA. Validation: No CGH probes for gk1086."
Proper citation: RRID:WB-STRAIN:WBStrain00037305 Copy
http://www.wormbase.org/db/get?name=WBStrain00037306
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003167(mec-3)
Genomic Alteration: WBGene00003167(mec-3)
Availability: available
References:
Synonyms: mec-3(gk1126) IV.
Alternate IDs: WB-STRAIN:VC2396, CGC_VC2396
Notes: F01D4.6. Identified by PCR, validated by CGH. External left primer: CGCGTTGAAGTCAGTTGTGT. External right primer: GACTCCTGTTGGATTGGCAT. Internal left primer: CTGCCACATCAGTGTTGCTT. Internal right primer: CAAAGCCTCTCAGTGCGATT. Internal WT amplicon: 2450 bp. Deletion size: 774 bp. Deletion left flank: GAGCAAAGCGTAAAAAATGATTACATCTTT. Deletion right flank: TTTTACTTGACTCTCTGAAAGTCGAACAGA. Insertion Sequence: TTACTT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037306 Copy
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