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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00035236
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: oaSi37 II; unc-119(ed3) III.
Alternate IDs: WB-STRAIN:UE95, CGC_UE95
Notes: oaSi37 [par-5p::par-5(partially recoded)::par-5 3' UTR(mutated proximal poly(A)site) + unc-119(+)] II. MOS single copy insertion of PAR-5 under control of the long PAR-5 3'UTR isoforms (utr.1 and utr.2) to test gene dosage control and investigate crossregulation between the two par-5 loci. Reference: Mikl, M. and Cowan, CR. Cell Rep. 2014 Sep 11;8(5):1380-90.
Proper citation: RRID:WB-STRAIN:WBStrain00035236 Copy
http://www.wormbase.org/db/get?name=WBStrain00035235
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: oaSi34 II; unc-119(ed3) III.
Alternate IDs: WB-STRAIN:UE92, CGC_UE92
Notes: oaSi34 [par-5p::par-5(partially recoded)::par-5 3' UTR(mutated splice sites, mutated proximal poly(A)site) + unc-119(+)] II. MOS single copy insertion of PAR-5 under control of the PAR-5 3'UTR.1 isoform exclusively to test gene dosage control and investigate crossregulation between the two par-5 loci. Reference: Mikl, M. and Cowan, CR. Cell Rep. 2014 Sep 11;8(5):1380-90.
Proper citation: RRID:WB-STRAIN:WBStrain00035235 Copy
http://www.wormbase.org/db/get?name=WBStrain00035386
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; leEx1606.
Alternate IDs: WB-STRAIN:UL1606, CGC_UL1606
Notes: leEx1606 contains [aha-1::GFP + unc-119(+)]. Trangenic animals are superficially wild-type. Maintain by picking GFP+.
Proper citation: RRID:WB-STRAIN:WBStrain00035386 Copy
http://www.wormbase.org/db/get?name=WBStrain00035395
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; leEx1709.
Alternate IDs: WB-STRAIN:UL1709, CGC_UL1709
Notes: leEx1709 [ahr-1::GFP + unc-119(+)]. Superficially wild-type. Grove C. A., De Masi F., et al (2009) Cell 138(2); 314-327.
Proper citation: RRID:WB-STRAIN:WBStrain00035395 Copy
http://www.wormbase.org/db/get?name=WBStrain00035394
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; leEx1692.
Alternate IDs: WB-STRAIN:UL1692, CGC_UL1692
Notes: leEx1692 contains [hlh-34::GFP + unc-119(+)]. Trangenic animals are superficially wild-type. Maintain by picking GFP+.
Proper citation: RRID:WB-STRAIN:WBStrain00035394 Copy
http://www.wormbase.org/db/get?name=WBStrain00035375
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; leEx1435.
Alternate IDs: WB-STRAIN:UL1435, CGC_UL1435
Notes: leEx1435 contains [hnd-1::GFP + unc-119(+)]. Trangenic animals are superficially wild-type. Maintain by picking GFP+.
Proper citation: RRID:WB-STRAIN:WBStrain00035375 Copy
http://www.wormbase.org/db/get?name=WBStrain00035374
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; leEx1432.
Alternate IDs: WB-STRAIN:UL1432, CGC_UL1432
Notes: leEx1432 [hlh-10::GFP + unc-119(+)]. Superficially wildtype. Grove C. A., De Masi F., et al (2009) Cell 138(2); 314-327.
Proper citation: RRID:WB-STRAIN:WBStrain00035374 Copy
http://www.wormbase.org/db/get?name=WBStrain00035379
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; leEx1531.
Alternate IDs: WB-STRAIN:UL1531, CGC_UL1531
Notes: leEx1531 contains [mml-1::GFP + unc-119(+)]. Trangenic animals are superficially wild-type. Maintain by picking GFP+.
Proper citation: RRID:WB-STRAIN:WBStrain00035379 Copy
http://www.wormbase.org/db/get?name=WBStrain00035378
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; leEx1447.
Alternate IDs: WB-STRAIN:UL1447, CGC_UL1447
Notes: leEx1447 contains [hif-1::GFP + unc-119(+)]. Trangenic animals are superficially wild-type. Maintain by picking GFP+.
Proper citation: RRID:WB-STRAIN:WBStrain00035378 Copy
http://www.wormbase.org/db/get?name=WBStrain00035381
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; leEx1546.
Alternate IDs: WB-STRAIN:UL1546, CGC_UL1546
Notes: leEx1546 [hlh-2::GFP + unc-119(+)]. Superficially wildtype. Grove C. A., De Masi F., et al (2009) Cell 138(2); 314-327.
Proper citation: RRID:WB-STRAIN:WBStrain00035381 Copy
http://www.wormbase.org/db/get?name=WBStrain00035383
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; leEx1566.
Alternate IDs: WB-STRAIN:UL1566, CGC_UL1566
Notes: leEx1566 [lin-32::GFP + unc-119(+)]. Superficially wildtype. Grove C. A., De Masi F., et al (2009) Cell 138(2); 314-327.
Proper citation: RRID:WB-STRAIN:WBStrain00035383 Copy
http://www.wormbase.org/db/get?name=WBStrain00035460
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; jfIs2.
Alternate IDs: WB-STRAIN:UV7, CGC_UV7
Notes: jfIs2[pie-promoter::GFP::zhp-3 + unc-119(+)]. Maintain at 15C.
Proper citation: RRID:WB-STRAIN:WBStrain00035460 Copy
http://www.wormbase.org/db/get?name=WBStrain00035422
Source Database: WormBase (WB)
Affected Genes: WBGene00002992(lin-3)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00002992(lin-3), WBGene00006843(unc-119)
Availability: available
Synonyms: csSi3 [lin-3::lin-3S + unc-119(+)] II; lin-3(n1059) IV/nT1[qIs51] (IV;V)
Alternate IDs: WB-STRAIN:UP2813, CGC_UP2813
Notes: lin-3(-) heterozygous balanced strain containing single copy MOS-mediated insertion of csSi1 transgene encoding lin-3S (short) splice isoform, expressed under control of the lin-3 promoter. The transgene rescues lethal and Vulvaless phenotypes (but not sterility) of lin-3 mutants, which can be recognized by absence of myo-2::GFP from the nT1 balancer. Pick GFP+ to maintain.
Proper citation: RRID:WB-STRAIN:WBStrain00035422 Copy
http://www.wormbase.org/db/get?name=WBStrain00035424
Source Database: WormBase (WB)
Affected Genes: WBGene00002992(lin-3)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00002992(lin-3), WBGene00006843(unc-119)
Availability: available
Synonyms: csSi2 [lin-3::lin-3XL + unc-119(+)] II; lin-3(n1059) IV/nT1[qIs51] (IV;V)
Alternate IDs: WB-STRAIN:UP2815, CGC_UP2815
Notes: lin-3(-) heterozygous balanced strain containing single copy MOS-mediated insertion of csSi1 transgene encoding lin-3XL (extra long) splice isoform, expressed under control of the lin-3 promoter. The transgene partially rescues lethality (but not Vulvaless or sterile phenotypes) of lin-3 mutants, which can be recognized by absence of myo-2::GFP from the nT1 balancer. Pick GFP+ to maintain.
Proper citation: RRID:WB-STRAIN:WBStrain00035424 Copy
http://www.wormbase.org/db/get?name=WBStrain00049949
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:33027250, PMID:37782660
Synonyms: ijmSi3 I; unc-119(ed3) III; ltIs37 IV.
Notes: Generated from papers flagged positive during the last month for data type afp_otherstrain and contains genotype and gene data.|"ijmSi3 [mex-5p::cls-2(re-encoded)::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] I. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. The coding sequence of GFP-tagged cls-2 in the transgene was re-coded using silent mutations to render it insensitive to RNAi-depletion of endogenous cls-2 expression. mCherry expression marks histones. Not known if unc-119(ed3) is still carried in the background of this strain. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Schlientz A and Bowerman B. PLoS Genet. 2020 Oct 7;16(10):e1008751. doi: 10.1371/journal.pgen.1008751."|"Made_by: Aleesa Schlientz"|"Supplementary_genotype ijmSi3 [pJD342/pJD330; ChrI_5mex-5_cls-2reenc::GFP_3tbb-2; cb-unc-119(+)]I; unc-119(ed3) III?; itIs37[pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV"
Proper citation: RRID:WB-STRAIN:WBStrain00049949 Copy
http://www.wormbase.org/db/get?name=WBStrain00050174
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:34003969
Synonyms: vsSi32 unc-119(ed3) III.
Notes: Generated from papers flagged positive during the last month for data type afp_otherstrain and contains genotype and gene data.|"vsSi32 [goa-1::GFP + unc-119(+)] III. GOA-1::GFP translational fusion driven by 5kb of goa-1 promoter. Rescues unc-119 locomotion defect. vsSi32 is inserted into the left arm of chromosome III between sequences 5-tttactgcatactgaacaacaggggaaaagggg-3 and 5-tagaattagctgtaagacggcgtctaggttttgca-3. Reference: Kumar S, et al. G3 (Bethesda). 2021 Aug 7;11(8):jkab167. doi: 10.1093/g3journal/jkab167. PMID: 34003969"
Proper citation: RRID:WB-STRAIN:WBStrain00050174 Copy
http://www.wormbase.org/db/get?name=WBStrain00050636
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: antIs30 II; unc-119(ed9) III.
Notes: antIs30 [attP-f + Cbr-unc-119(+) + glh-2p::phiC31 + rol-6(partial) + myo-2p::GFP + attP-r] II. antIs30 was inserted into ttTi5605 on LG II using MosSCI. GFP expression in pharynx is very weak (as it is single copy) and is easiest to see during the L1-L3 stages. This strain contains a phiC31 docking site and can be used for precise single-copy integration of transgenes via recombination mediated cassette exchange. The docking site contains inverted phiC31-attP sites flanking phiC31 integrase expressed from the glh-2 germline promoter. Integration constructs need to have inverted phiC31-attB sites that flank the intended sequence to be inserted. Reference: Yang FJ, et al. phiC31 integrase for recombination mediated single copy insertion and genome manipulation in C. elegans. Genetics 2021.|"Made_by: Fang-Jung Yang"
Proper citation: RRID:WB-STRAIN:WBStrain00050636 Copy
http://www.wormbase.org/db/get?name=WBStrain00050674
Source Database: WormBase (WB)
Affected Genes: WBGene00001067(dpy-5)|WBGene00002225(klp-15)|WBGene00002226(klp-16)|WBGene00004027(pie-1)|WBGene00006843(unc-119)|WBGene00008107(aspm-1)
Genomic Alteration: WBGene00001067(dpy-5), WBGene00002225(klp-15), WBGene00002226(klp-16), WBGene00004027(pie-1), WBGene00006843(unc-119), WBGene00008107(aspm-1)
Availability: unknown
Synonyms: klp-15(ok1958) aspm-1(syb1260[gfp::aspm-1]) klp-16(or1952) /tmC18[dpy-5(tmIs1236)] I; ltIs37[pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV
Notes: itIs37 [pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. GFP tag inserted into endogenous aspm-1 locus. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry triple mutant homozygotes. Homozygous triple mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020|"ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. GFP tag inserted into endogenous aspm-1 locus. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry triple mutant homozygotes. Homozygous triple mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020"
Proper citation: RRID:WB-STRAIN:WBStrain00050674 Copy
http://www.wormbase.org/db/get?name=WBStrain00050678
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: gwIs39 gwIs34 unc-119(ed3) III.
Notes: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. gwIs34 [myo-3::mCherry + 256xlacO + unc-119(+)] III. Superficially wild-type. Expresses GFP-LacI throughout development from early embryogenesis, forming a small spot at the lacO array. Worms have red muscle (from L1 stage) and green intestine (from late L4 stage). Reference: Meister P, et al. Genes Dev. 2010 Apr 15;24(8):766-82. PMID: 20395364
Proper citation: RRID:WB-STRAIN:WBStrain00050678 Copy
http://www.wormbase.org/db/get?name=WBStrain00050639
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:39652010
Synonyms: muIs252 II; unc-119(ed3) III.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Somatic expression of wrmScarlet1-10 (under the control of the eft-3 promoter and unc-54 3'UTR). Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628
Proper citation: RRID:WB-STRAIN:WBStrain00050639 Copy
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