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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00055057
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: hsp-12.6(syb1364[hsp-12.6::mKate2]) IV.
Notes: Made_by: SunyBiotech|"mKate2 tag was inserted into the endogenous hsp-12.6 locus using CRISPR/Cas9. HSP-12.6::mKate2 expression most visible in the muscles. Reference: Koutsoumparis A, et al. Curr Biol. 2022 Apr 26;S0960-9822(22)00581-4. doi: 10.1016/j.cub.2022.04.012. PMID: 35504281"
Proper citation: RRID:WB-STRAIN:WBStrain00055057 Copy
http://www.wormbase.org/db/get?name=WBStrain00055054
Source Database: WormBase (WB)
Affected Genes: WBGene00004010(pha-1)
Genomic Alteration: WBGene00004010(pha-1)
Availability: unknown
Source References: EMPTY
Synonyms: pha-1(e2123) III; otEx8047.
Notes: Made_by: Daniel Merritt|"otEx8047 [pks-1p::TagRFP + pha-1(+)]. Maintain at 25C to select for array. TagRFP expression marks CAN neurons."
Proper citation: RRID:WB-STRAIN:WBStrain00055054 Copy
http://www.wormbase.org/db/get?name=WBStrain00055055
Source Database: WormBase (WB)
Affected Genes: WBGene00000464(ceh-44)|WBGene00006807(unc-75)
Genomic Alteration: WBGene00000464(ceh-44), WBGene00006807(unc-75)
Availability: unknown
Source References: EMPTY
Synonyms: unc-75(ot1351) I; ceh-44(ot1015[ceh-44::GFP]) III.
Notes: Made_by: Michael Cesar|"ot1351 is a CRISPR-engineered deletion of unc-75 gene rendering all 3 RNA recognition motifs null on unc-75; reduces pan-neuronal nuclear GFP expression fluorescence. GFP tag inserted at the C-terminus of the endogenous ceh-44 locus by CRISPR. Please contact Oliver Hobert prior to publishing work using this strain."
Proper citation: RRID:WB-STRAIN:WBStrain00055055 Copy
http://www.wormbase.org/db/get?name=WBStrain00055061
Source Database: WormBase (WB)
Affected Genes: WBGene00003386(mod-1)
Genomic Alteration: WBGene00003386(mod-1)
Availability: unknown
Source References: EMPTY
Synonyms: mod-1(syb1841[mod-1::T2A::mNeonGreen]) V.
Notes: Endogenous mod-1 locus tagged with mNeonGreen. Inclusion of the T2A self-cleaving peptide allows mNeonGreen406to be expressed as a cytosolic protein. Generated in N2 background. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https:|"Made_by: Steve Flavell"
Proper citation: RRID:WB-STRAIN:WBStrain00055061 Copy
http://www.wormbase.org/db/get?name=WBStrain00055046
Source Database: WormBase (WB)
Affected Genes: WBGene00005016(sqt-1)|WBGene00006537(tbb-2)|WBGene00194659(linc-1)
Genomic Alteration: WBGene00005016(sqt-1), WBGene00006537(tbb-2), WBGene00194659(linc-1)
Availability: unknown
Source References: EMPTY
Synonyms: linc-1(ot1249[linc-1p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) I.
Notes: Made_by: Sandeep Wontakal|"The Null Transcriptional Reporter (NuTR) cassette was used to remove the linc-1 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. GFP expression is seen in the male somatic gonad."
Proper citation: RRID:WB-STRAIN:WBStrain00055046 Copy
http://www.wormbase.org/db/get?name=WBStrain00055049
Source Database: WormBase (WB)
Affected Genes: WBGene00011141(nlp-66)
Genomic Alteration: WBGene00011141(nlp-66)
Availability: unknown
Source References: EMPTY
Synonyms: otIs669 V; nlp-66(syb4403[nlp-66:SL2:GFP::H2B])X.
Notes: GFP tag inserted at the C-terminus of the endogenous nlp-66 locus by CRISPR. Allele generated by SUNY Biotech. See description of strain OH15262 for full description of otIs669 NeuroPAL (Neuronal Polychromatic Atlas of Landmarks) transgene (Yemini E, et al. Cell. 2021 Jan 7;184(1):272-288.e11. PMID: 33378642). Reference: Reilly MB, et al. bioRxiv 2022.04.29.490095; doi: https:|"Made_by: Suny Biotech"
Proper citation: RRID:WB-STRAIN:WBStrain00055049 Copy
http://www.wormbase.org/db/get?name=WBStrain00055042
Source Database: WormBase (WB)
Affected Genes: WBGene00004245(puf-9)
Genomic Alteration: WBGene00004245(puf-9)
Availability: unknown
Source References: EMPTY
Synonyms: puf-9(ot1236[puf-9::GFP::loxP::3xFLAG]) X.
Notes: Made_by: Sandeep Wontakal|"SEC cassette was used to generate a C-terminal GFP tag of puf-9. Expression is cytoplasmic and pan-somatic throughout larval development into adults."
Proper citation: RRID:WB-STRAIN:WBStrain00055042 Copy
http://www.wormbase.org/db/get?name=WBStrain00055050
Source Database: WormBase (WB)
Affected Genes: WBGene00006652(ttx-1)
Genomic Alteration: WBGene00006652(ttx-1)
Availability: unknown
Source References: EMPTY
Synonyms: ttx-1(syb1679[ttx-1::GFP]) ot1264) V.
Notes: Made_by: Berta Vidal (Hobert lab)|"ot1264 is a CRISPR deletion removing -10.8 kb to -1.8 kb before the first exon of ttx-1, made in the context of the ttx-1::GFP allele syb1679. Notably ttx-1 expression in RIB is lost, and RIB markers are off or dim. Reference: Reilly MB, et al. Widespread employment of conserved C. elegans homeobox genes in neuronal identity specification. bioRxiv 2022.04.29.490095; doi: https:"
Proper citation: RRID:WB-STRAIN:WBStrain00055050 Copy
http://www.wormbase.org/db/get?name=WBStrain00055052
Source Database: WormBase (WB)
Affected Genes: WBGene00004010(pha-1)
Genomic Alteration: WBGene00004010(pha-1)
Availability: unknown
Source References: EMPTY
Synonyms: pha-1(e2123) III; otIs355; otEx7950.
Notes: Made_by: Wendy Cao|"otIs355 [rab-3p(prom1)::2xNLS::TagRFP] IV. otEx7950 [clec-166::GFP + pha-1(+)]. Maintain at 25C to select for animals carrying the array. Pan-neuronal nuclear RFP expression. Co-expression of GFP and TagRFP in M5 neuron can be used to isolate M5 by FACS. Used by CeNGEN project for RNA-Seq (https:"
Proper citation: RRID:WB-STRAIN:WBStrain00055052 Copy
http://www.wormbase.org/db/get?name=WBStrain00055039
Source Database: WormBase (WB)
Affected Genes: WBGene00005016(sqt-1)|WBGene00006537(tbb-2)|WBGene00219694(linc-36)
Genomic Alteration: WBGene00005016(sqt-1), WBGene00006537(tbb-2), WBGene00219694(linc-36)
Availability: unknown
Source References: EMPTY
Synonyms: linc-36(ot1229[linc-36p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) IV.
Notes: Made_by: Sandeep Wontakal|"The Null Transcriptional Reporter (NuTR) cassette was used to remove the linc-36 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. GFP expression is seen in the sperm of both hermaphrodites and males."
Proper citation: RRID:WB-STRAIN:WBStrain00055039 Copy
http://www.wormbase.org/db/get?name=WBStrain00055041
Source Database: WormBase (WB)
Affected Genes: WBGene00005016(sqt-1)|WBGene00006537(tbb-2)|WBGene00006650(tts-1)
Genomic Alteration: WBGene00005016(sqt-1), WBGene00006537(tbb-2), WBGene00006650(tts-1)
Availability: unknown
Source References: EMPTY
Synonyms: tts-1(ot1234[tts-1p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) X.
Notes: Made_by: Sandeep Wontakal|"The Null Transcriptional Reporter (NuTR) cassette was used to remove the tts-1 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. GFP expression is seen in several tissues including the pharynx but is significantly upregulated in a stress-dependent manner in a variety of tissues."
Proper citation: RRID:WB-STRAIN:WBStrain00055041 Copy
http://www.wormbase.org/db/get?name=WBStrain00055040
Source Database: WormBase (WB)
Affected Genes: WBGene00005016(sqt-1)|WBGene00006537(tbb-2)|WBGene00219689(linc-41)
Genomic Alteration: WBGene00005016(sqt-1), WBGene00006537(tbb-2), WBGene00219689(linc-41)
Availability: unknown
Source References: EMPTY
Synonyms: linc-41(ot1231[linc-41p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) IV.
Notes: Made_by: Sandeep Wontakal|"The Null Transcriptional Reporter (NuTR) cassette was used to remove the linc-41 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. GFP expression is seen in the male somatic gonad, spermatheca of hermaphrodites, as well as several other tissues in the head."
Proper citation: RRID:WB-STRAIN:WBStrain00055040 Copy
http://www.wormbase.org/db/get?name=WBStrain00055124
Source Database: WormBase (WB)
Affected Genes: WBGene00001135(eat-4)
Genomic Alteration: WBGene00001135(eat-4)
Availability: unknown
Source References: EMPTY
Synonyms: eat-4(syb4257[eat-4::T2A::GFP::H2B]) III.
Notes: Endogenous eat-4 locus tagged with T2A::GFP::H2B. Healthy strain that has all glutamatergic neurons marked with GFP. Reference: Vidal B, et al. Elife. 2022 Mar 24;11:e76003. doi: 10.7554/eLife.76003. PMID: 35324425|"Made_by: Suny Biotech"
Proper citation: RRID:WB-STRAIN:WBStrain00055124 Copy
http://www.wormbase.org/db/get?name=WBStrain00055123
Source Database: WormBase (WB)
Affected Genes: WBGene00001463(flp-20)
Genomic Alteration: WBGene00001463(flp-20)
Availability: unknown
Source References: PMID:37935195
Synonyms: flp-20(syb4049[flp-20::SL2::GFP::H2B]) X.
Notes: GFP tag inserted at the C-terminus of the endogenous flp-20 locus by CRISPR. Allele generated by SUNY Biotech. Please contact Oliver Hobert prior to publishing work using this strain.|"GFP tag inserted at the C-terminus of the endogenous flp-20 locus by CRISPR. Allele generated by SUNY Biotech. Reference: Ripoll-Sanchez L, et al. Neuron. 2023 Nov 15;111(22):3570-3589.e5. doi: 10.1016/j.neuron.2023.09.043. PMID: 37935195."|"Made_by: Suny Biotech"|"Supplementary_genotype flp-20(syb4049[flp-20::SL2::GFP::H2B]) X"
Proper citation: RRID:WB-STRAIN:WBStrain00055123 Copy
http://www.wormbase.org/db/get?name=WBStrain00055125
Source Database: WormBase (WB)
Affected Genes: WBGene00010982(flp-32)
Genomic Alteration: WBGene00010982(flp-32)
Availability: unknown
Source References: PMID:37935195
Synonyms: flp-32(syb4374[flp-32::SL2::gfp::H2B])X.
Notes: CRISPR/Cas9 engineered tagged endogenous locus. Reference: Cros C & Hobert O. Proc Natl Acad Sci USA. 2022 Sep 13;119(37):e2206817119. doi: 10.1073/pnas.2206817119. PMID: 36067313.|"CRISPR/Cas9 engineered tagged endogneous locus. Reference: Cros C & Hobert O. bioRxiv 2022.04.19.488792; doi: https:"|"Made_by: Suny Biotech"|"Supplementary_genotype flp-32(syb4374[flp-32::SL2::gfp::H2B]) X"
Proper citation: RRID:WB-STRAIN:WBStrain00055125 Copy
http://www.wormbase.org/db/get?name=WBStrain00055120
Source Database: WormBase (WB)
Affected Genes: WBGene00000240(pah-1)
Genomic Alteration: WBGene00000240(pah-1)
Availability: unknown
Source References: EMPTY
Synonyms: pah-1(syb3634[GFP::H2B::T2A::pah-1]) II.
Notes: Made_by: Frank Schroeder via SUNY Biotech|"Superficially wild-type. GFP tag inserted into endogenous pah-1 locus by CRISPR/Cas9."
Proper citation: RRID:WB-STRAIN:WBStrain00055120 Copy
http://www.wormbase.org/db/get?name=WBStrain00055086
Source Database: WormBase (WB)
Affected Genes: WBGene00022276(nlp-40)
Genomic Alteration: WBGene00022276(nlp-40)
Availability: unknown
Source References: EMPTY
Synonyms: nlp-40(syb3208 [nlp-40::T2A::3XNLS::GFP]) I.
Notes: GFP tag inserted at the C-terminus of the endogenous nlp-40 locus by CRISPR. Allele generated by SUNY Biotech. Please contact Oliver Hobert prior to publishing work using this strain.|"Made_by: Suny Biotech"
Proper citation: RRID:WB-STRAIN:WBStrain00055086 Copy
http://www.wormbase.org/db/get?name=WBStrain00055089
Source Database: WormBase (WB)
Affected Genes: WBGene00001452(flp-9)
Genomic Alteration: WBGene00001452(flp-9)
Availability: unknown
Source References: EMPTY
Synonyms: flp-9(syb3229[flp-9::T2A::3xNLS::GFP]) IV.
Notes: CRISPR/Cas9 engineered tagged endogenous locus.|"CRISPR/Cas9 engineered tagged endogneous locus."|"Made_by: Suny Biotech"
Proper citation: RRID:WB-STRAIN:WBStrain00055089 Copy
http://www.wormbase.org/db/get?name=WBStrain00055122
Source Database: WormBase (WB)
Affected Genes: WBGene00011997(nlp-51)
Genomic Alteration: WBGene00011997(nlp-51)
Availability: unknown
Source References: PMID:37935195
Synonyms: nlp-51(syb3936[nlp-51::SL2::GFP::H2B]) II.
Notes: GFP tag inserted at the C-terminus of the endogenous nlp-51 locus by CRISPR. Allele generated by SUNY Biotech. Reference: Reilly MB, et al. bioRxiv 2022.04.29.490095; doi: https:|"GFP tag inserted at the C-terminus of the endogenous nlp-51 locus by CRISPR. Allele generated by SUNY Biotech. Reference: Reilly MB, et al. PLoS Genet. 2022 Sep 30;18(9):e1010372. doi: 10.1371/journal.pgen.1010372. PMID: 36178933."|"Made_by: Suny Biotech"|"Supplementary_genotype nlp-51(syb3936[nlp-51::SL2::GFP::H2B]) II"
Proper citation: RRID:WB-STRAIN:WBStrain00055122 Copy
http://www.wormbase.org/db/get?name=WBStrain00055088
Source Database: WormBase (WB)
Affected Genes: WBGene00003755(nlp-17)
Genomic Alteration: WBGene00003755(nlp-17)
Availability: unknown
Source References: EMPTY
Synonyms: nlp-17(syb3222[nlp-17::T2A::3NLS::GFP]) IV.
Notes: Endogenous locus tagged with T2A::3xNLS::GFP using CRISPR/Cas9. Please contact Oliver Hobert prior to publishing work using this strain.|"Made_by: Suny Biotech"
Proper citation: RRID:WB-STRAIN:WBStrain00055088 Copy
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