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SciCrunch Registry is a curated repository of scientific resources, with a focus on biomedical resources, including tools, databases, and core facilities - visit SciCrunch to register your resource.
http://cushaw2.sourceforge.net/homepage.htm#latest
Software package for next-generation sequencing read alignment that is fast and parallel gapped read alignment to large genomes, such as the human genome.
Proper citation: CUSHAW (RRID:SCR_005479) Copy
http://ngsview.sourceforge.net/
A generally applicable, flexible and extensible next-generation sequence alignment editor. The software allows for visualization and manipulation of millions of sequences simultaneously on a desktop computer, through a graphical interface.
Proper citation: NGSView (RRID:SCR_005637) Copy
http://samtools.sourceforge.net/tview.shtml
Text alignment viewer software based on the GNU ncurses library that works with short indels and shows MAQ consensus. It uses different colors to display mapping quality or base quality, subjected to users' choice.
Proper citation: SAMtools Text Alignment Viewer (RRID:SCR_005611) Copy
http://sourceforge.net/projects/cancergrid-tma/
A web-based application for the management and storage of tissue microarray (TMA) images and the associated metadata. The application enables the user to navigate a grid of TMA core images within a slide, zoom and pan around an image, and enter a score constrained to a specific scoring system. The submitted scores are scored in the eXist open source database, in an XML format, which is compatible with existing TMA standards, and thus allow the data to be archived and re-used in future analysis.
Proper citation: cancergrid-tma (RRID:SCR_005595) Copy
A web-based software package for comparative genomics.
Proper citation: Sybil (RRID:SCR_005593) Copy
http://sourceforge.net/projects/dna-bison/
Allows users with access to a computer cluster to rapidly align whole-genome bisulfite sequencing or RRBS reads.
Proper citation: dna-bison (RRID:SCR_005913) Copy
http://sourceforge.net/projects/cohcap/
An algorithm to analyze single-nucleotide resolution methylation data (Illumina 450k methylation array, targeted BS-Seq, etc.). It provides QC metrics, differential methylation for CpG Sites, differential methylation for CpG Islands, integration with gene expression data, and visualization of methylation values.
Proper citation: COHCAP (RRID:SCR_006499) Copy
A web-based software tool offering an integrated analysis of transcriptome data under genomic, proteomic and metabolic context.
Proper citation: GEPAT (RRID:SCR_003597) Copy
http://sourceforge.net/projects/insertionmapper/
A pipeline tool for the identification of targeted sequences from multidimensional high throughput sequencing data. It consists of four independently working modules: Data Preprocessing, Database Modeling, Dimension Deconvolution and Element Mapping. This pipeline tool is applicable to scenarios requiring analysis of the tremendous output of short reads produced in NGS sequencing experiments of targeted genome sequences.
Proper citation: InsertionMapper (RRID:SCR_004163) Copy
http://cb-commander.sourceforge.net/pmwiki.php
A plugin based software tool that tries to integrate high throughput sequencing algorithms. It allows researchers to design and execute their experiments through a user friendly interface, enabling users to integrate di erent components of an experiment, e.g. algorithms and converters, into one graphically interfaced application that is very easy to use when working on remote servers as well as local computers. The graphical user interface facilitates a visual design of experiments by using a block diagram to represent the components (algorithms, converters, etc.) of an experiment as a pipeline. The users can easily modify this pipeline.
Proper citation: CB-Commander (RRID:SCR_004237) Copy
http://rubioseq.sourceforge.net/
Stand-alone and multiplatform application for the integrated analysis of NGS data. It implements pipelines for the analysis of single nucleotide and copy-number variation and bisulfite-seq and ChIP-seq experiments.
Proper citation: RUbioSeq (RRID:SCR_002508) Copy
http://sourceforge.net/projects/b-o-s-s/
Batch primer selection software program designed to select PCR oligos for gap closure for assemblies containing a large number of gaps. It will select oligos for gap closure of both contig and scaffold gaps.
Proper citation: Batch Oligo Selection Script (RRID:SCR_002808) Copy
http://sourceforge.net/projects/mipe/
A XML format that enables genomics researchers to store critical information on PCR experiments. Accompagnying perl scripts are written to read from (dbSTS) or write to a MIPE XML file.
Proper citation: MIPE (RRID:SCR_003065) Copy
http://mrsfast.sourceforge.net/
A cache-oblivious algorithm designed to map short reads to reference genome assemblies in a fast and memory-efficient manner. It optimizes cache usage to get higher performance. Currently Supported Features: * Mistmatches, No indels * Paired-end Mapping Mode * Discordant Paired-end Mapping Mode (to be used in conjuction with Variation Hunter)
Proper citation: mrsFAST (RRID:SCR_003128) Copy
http://splicq.sourceforge.net/
A Java software package which allows for the identification of splicing events and differentially expressed isoforms in next generation sequencing data.
Proper citation: SpliCQ (RRID:SCR_003266) Copy
http://sourceforge.net/projects/orfer/
An extended software package for high throughput PCR primer design for biological sequences. It reads the NCBI GenBank XML sequence format and extracts open reading frames for proteins. Sequences can be requested by GI or accession number.
Proper citation: ORFprimer (RRID:SCR_003269) Copy
http://sourceforge.net/projects/gemi/
Automated software tool to design polymerase chain reaction (PCR) primers. It accepts multiple aligned and long sequences with degenerated nucleotides. It can be used for quantitative/real-time PCR, conventional and Sanger sequencing. Gemi accepts DNA and RNA sequences with degenerate nucleotide (non-A/C/G/T bases). The programs are as the following: # The first program is to design PCR primers from multiple sequence alignment. # Program to convert ClustalW format (.aln), Phylip (.phy) and (.gde) formats to Fasta format. # Reverse and/or complement program is to find the reverse and complement counterpart of single or multiple sequences.
Proper citation: Gemi (RRID:SCR_003211) Copy
A complete software system used to store and analyze gene expression data.
Proper citation: GEOSS (RRID:SCR_003401) Copy
http://sourceforge.net/projects/amplicon/
Software tool for designing PCR primers on aligned groups of DNA sequences. The most important application is the design of "group-specific" PCR primer sets that amplify a DNA region from a given taxonomic group but do not amplify orthologous regions from other taxonomic groups. It is written in Python 2.3 and Tkinter 8.4. The current script was created for Windows and an executable is available. Future versions of the script should be able to run on Linux and Mac
Proper citation: Amplicon (RRID:SCR_003294) Copy
http://sourceforge.net/projects/pasha/
A parallel short read assembler for large genomes using de Bruijn graphs.
Proper citation: PASHA (RRID:SCR_004455) Copy
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