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Genome Access Technology Center at the McDonnell Genome Institute offers comprehensive next generation sequencing, microarray, PCR and Bioinformatic services. In addition to generating high quality genomic, transcriptomic, and proteomic data, performs data analysis and provides technological support to users. Full service facility, from hypothesis to publication. Offers advanced analysis of microarray data. Provides free initial consultation to discuss project and offers several tiers of analysis packages to best suit your needs. NGS equipment includes NovaSeqs, Seqwell II, Oxford. Microarray expression and genotyping - all platforms.
Proper citation: Washington University School of Medicine Genome Technology Access Center Core Facility (RRID:SCR_001030) Copy
https://nyumc.ilab.agilent.com/service_center/4273
Core offers services for researchers who want to apply advanced molecular genetic techniques in rodent models of physiology and disease. Provides expertise in generating novel mutant and transgenic mouse strains using genome engineering in mouse embryos and in embryonic stem cells (ESCs). Available technologies include:Generation of genome-edited mice by embryo pronuclear microinjection of DNA and genome editors (e.g., CRISPR/Cas9, site-specific recombinases) or traditional BAC transgenesis;Generation of genome-edited mice from mouse embryonic stem cells (mESCs) by chimeric blastocyst injection;Generation of genome-edited mice from mESCs by tetraploid blastocyst injection; Generation of mice from induced pluripotent stem cells;Assisted reproductive technologies; Sperm and embryo cryopreservation, storage and import/export;in vitro fertilization (IVF); Embryo rederivation technologies for animal import into barrier vivaria through quarantine.
Proper citation: NYU Langone’s Advanced Rodent Transgenics Laboratory ART-Lab Core Facility (RRID:SCR_017692) Copy
https://www.ohsu.edu/dna-services-core
Services offered include Human cell line authentication: Accurate validation of human cell lines using STR analysis and meeting the ANSI/ATCC requirements; Fragment analysis: Detection of changes in length of specific DNA sequence to indicate presence or absence of genetic marker; Oligo synthesis: Creation of custom primers and probes for oligo and siRNA synthesis through Integrated DNA Technologies (IDT); DNA quantification: Quantification, size and quality assessment of DNA samples; Consulting: For project design and sample preparation assistance.
Proper citation: OHSU DNA Services Core Facility (RRID:SCR_009968) Copy
https://www.med.upenn.edu/scxc/
Offers in vivo services specializing in immunodeficient and xenograft models (PDX, humanized immune system). Facility has dedicated BSL2 barrier space equipped with optical imaging, for applications ranging from immunotherapy, cancer biology, infectious diseases and regenerative medicine. Offers services centered around repository of live and fully annotated cells from adult patients with hematologic malignancies (AML, ALL, MPN, MDS), and hematopoietic stem/progenitor cells from healthy donors (BM, CB, and FL).
Proper citation: Pennsylvania University Perelman School of Medicine Stem Cell and Xenograft Core Facility (RRID:SCR_010035) Copy
https://cancer.dartmouth.edu/scientists-researchers/clinical-pharmacology
Supports Dartmouth Cancer Center investigators in design, performance, data analysis, and interpretation of pharmacology objectives in preclinical, clinical and epidemiological studies.
Proper citation: Dartmouth College Clinical Pharmacology Shared Resource Core Facility (RRID:SCR_000873) Copy
https://www.colorado.edu/facility/ems/
Core provides access to instruments including:FEI Tecnai 12 Spirit TEM, FEI Tecnai F20 (200kV) FEG-TEM 200kV FEG-TEM,Gatan US4000 4k x 4k CCD, bottom-mount,CryoTEM and electron tomography,High-resolution TEM;FEI Tecnai F20 (200kV) FEG-TEM,300kV FEG-TEM,Gatan US4000 4k x 4k CCD, bottom-mount,CryoTEM and electron tomography,High-resolution TEM,FEI/Phillips CM100 (100kV) TEM,100kV, tungsten TEM,2k x 2k AMT CCD, bottom-mount.
Proper citation: Colorado University at Boulder EM Services Core Facility (RRID:SCR_001432) Copy
https://genome.med.harvard.edu/
Provided core services include Sanger DNA Sequencing, Next Generation DNA Sequencing (Four Illumina MiSeqs, four Illumina NextSeq 500s and one Illumina NovaSeq 6000),Next Generation Automated Library Sample Prep (Perkin Elmer Sciclone Genomic, ChIP, RNAseq and other methods), Single Cell DNA and RNA-Seq (10X Genomics Chromium), Bioanalyzer Analysis DNA and RNA (Two Agilent 2100 BioAnalyzers and two Agilent 2200 TapeStations), Qiagen Qiacube Automated DNA / RNA Prep (single to multiple samples), Oligonucleotide Ordering (IDT Portal), Reagents and Supplies Ordering (multiple vendors / products). Facility web based Laboratory Information Management System provides users with access to order services and supplies as well as retrieve data and review and pay invoices all online.
Proper citation: Harvard Medical School Biopolymers Core Facility (RRID:SCR_007175) Copy
https://biospherix.com/oxycycler-gt4181n/
Nitric Oxide, O2, CO2 controller with limit control of relative humidity and monitoring of temperature conditions when used in combination with C-Chamber incubator subchamber from BioSpherix. Features secondary monitor of Nitric Oxide and may be used in combination with I-Hood Incubator Hood from BioSpherix in order to ventilate fumes.
Proper citation: BioSpherix: Oxycycler GT4181N Controller (RRID:SCR_021112) Copy
https://www.coolled.com/products/pe-300white/
Compact and simple to use LED illuminator for microscopes. Provides broad spectrum of illumination, covering excitation bands of fluorophores such as DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more. Operation is by manual control pod with instant on and off, irradiance control, with remote control available via USB or TTL trigger.
Proper citation: CoolLED: pE-300white (RRID:SCR_021073) Copy
https://www.neurasmus.nl/index.php/products/blink-2-0
Scientific setup used for both eyeblink conditioning and prepulse inhibition of startle reflex in animals. Used to measure kinetic and frequency domain properties of conditioned and unconditioned eyelid responses in freely moving mice. Allows determination of eyelid movements in time and space while using air puff as unconditioned stimulus.
Proper citation: Neurasmus: BLINK system (RRID:SCR_021043) Copy
https://mdc.custhelp.com/euf/assets/content/Digidata_1322A_Manual.pdf
Digitizer intended for precision scientific applications.Digitizes at aggregate speed of 500 kHz. It provides sixteen multiplexed, 16-bit analog inputs and two nonmultiplexed, 16-bit analog outputs.
Proper citation: Molecular Devices: Digidata 1322A Digitizer (RRID:SCR_021041) Copy
https://neurophysics.ucsd.edu/Manuals/Axon%20Instruments/Digidata_1440A_Manual.pdf
Digitizer for electrophysiology experiments, to send and receive signals from microelectrode amplifiers, and to interact with peripheral instruments such as solution changers and stimulators. High resolution, low noise digitizer intended for precision scientific applications. Digidata 1440A digitizer communicates with the host computer using USB 2.0. It is plug and play device, so it is automatically recognized by Windows.
Proper citation: Molecular Devices: DigiData 1440 digitizer (RRID:SCR_021038) Copy
MEA amplifier for upright microscopes.Raw data from up to 60 electrodes of microelectrode array is amplified by 60 channels of filter amplifiers that are built very small and compact using Surface Mounted Devices technology.
Proper citation: Multichannel Systems: MEA60 Amplifier (RRID:SCR_021039) Copy
https://www.kloe-france.com/en/laser-lithography/photolithography-systems/uv-exposure/uv-kub-2
Compact exposure masking system is UV-LED masking system equipped with LED based optical head, collimated and homogeneous.One level mask aligner. Its masking function enables to reach resolutions less than 1um. This system is compatible with any of photoresists such as AZ series, Shipley series, SU-8 and K-CL series.
Proper citation: Kloe: UV-KUB 2 - UV-LED masking system (RRID:SCR_021141) Copy
https://andor.oxinst.com/products/scmos-camera-series/zyla-5-5-scmos
Large field of view, high resolution 5.5 megapixel sCMOS camera, ideal for research and OEM usage. Zyla 5.5 offers 100 fps frame rate and ultra low noise performance, in compact design.USB 3.0 version is available, offering up to 40 fps and industry fastest ROI speeds.
Proper citation: Andor: Zyla 5.5 sCMOS (RRID:SCR_021136) Copy
https://biospherix.com/i-glove/
Oxygen controlled Glovebox for both incubation and handling of cells in controlled physiologic or pathophysiologic conditions when used together with third party incubator. Carbon dioxide control is optional. Hosts C-Chamber Incubator Subchamber for transport of cells without exposure to room air.
Proper citation: BioSpherix: I Glove Incubator and Oxygen Glovebox chamber (RRID:SCR_021083) Copy
https://raw.githubusercontent.com/SciCrunch/RRID-Instruments/refs/heads/main/PDF/SCR_021097.pdf
Thermocycles with added control of VeriFlex technology, with six independent temperature blocks that provide precise control over PCR optimization. Color touch screen simplifies setup and use. Optional setups for fast or standard PCR methods provide with flexibility to shorten your PCR cycling times. Equipped with astandard 0.2 mL block configuration.Will be discontinued on December 31, 2021.
Proper citation: Thermo Fisher: Applied Biosystems: Veriti 96-Well Thermal Cycler (RRID:SCR_021097) Copy
http://pharmaspb.com/system/product/catalog_file/INCellAnalyzer2000/GE%20INCellAnalyzer2000.pdf
Cell imaging system for entire high content analysis (HCA) workflow. Delivers high performance hardware, software, and expert support for cell analysis.
Proper citation: GE: IN Cell Analyzer 2000 cell imaging system (RRID:SCR_021128) Copy
https://biospherix.com/oxycycler-at42co/
Provides either dynamic or static control of parts per million levels of carbon monoxide (CO) conditions in animal models modeling when used in combination with either one or two (1 or 2) A-Chamber Animal Chambers.
Proper citation: BioSpherix: OxyCycler AT42CO Controller (RRID:SCR_021186) Copy
https://biospherix.com/oxycycler-a44c/
Provides either dynamic or static control of CO2 conditions for animal modeling when used in combination with one to four (1-4) A-Chamber Animal Chambers purposes.
Proper citation: BioSpherix: OxyCycler A44C Controller (RRID:SCR_021184) Copy
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