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https://www.themiamiproject.org/research-resources/core-facilities/drug-discovery-core/
Core at Miami Project to Cure Paralysis leverages PerkinElmer Opera Phenix Plus high content screening system to provide assay development and phenotypic screening. Facility is also equipped with PerkinElmer Janus G3 liquid handler, featuring modular and variable dispense heads, to automate screening workflows and enhance throughput. Core supports phenotypic and target-based assays. Phenotypic assays range from 2D to 3D, widefield to confocal, and antibody to dye based. It collaborates with users to develop customized assays and analytical pipelines tailored to their specific needs. Current specializations include neurite tracing, lipid droplet quantification, cell viability, morphological and kinetic analyses, phagocytosis, cell marker detection, cell adhesion, among others.
Proper citation: Drug Discovery Core Facility (RRID:SCR_022542) Copy
Assists graduate students, staff and faculty launch, economize and expedite their research, especially when it involves DNA extraction and quality checks, targeted genotyping and DNA content analysis. Facilitates usage of complementary cores and assists with research planning and data analysis.AGL members have free lab access and reduced costs for services as well as one-on-one training and advising with AGL staff.
Proper citation: Texas A and M University AgriGenomics Laboratory Core Facility (RRID:SCR_022533) Copy
Facility offers genomics profiling services including RNAseq, small RNA sequencing, and ATACseq.
Proper citation: Cornell University Biotechnology Resource Center Transcriptional Regulation and Expression Core Facility (RRID:SCR_022532) Copy
https://www.cincinnatichildrens.org/research/cores/single-cell-genomics
Core to perform expression level analysis of thousands of genes in parallel.Uses RNA-Seq assays from Tecan Genomics, Takara and Illumina for samples with limited amounts of starting RNA. Microarray technology utilizing the Affymetrix GeneChip platform is also available.
Proper citation: Cincinnati Children's Hospital Single Cell Genomics Core Facility (RRID:SCR_022653) Copy
https://www.cincinnatichildrens.org/research/cores/cardio-imaging
Offers imaging techniques using imaging and measurement tools. Offers consulting services ranging from educational seminars to assistance with grant and industry funding support.
Proper citation: Cincinnati Children's Hospital Cardiovascular Imaging Core Facility (RRID:SCR_022626) Copy
Provides access to technologies and services for study of genomics and epigenomics of cancer, in addition to providing technical expertise for project design, trouble shooting and pre and post award support. Services include next generation sequencing, single cell genomics, spatial genomics, gene expression assays and molecular quantitation, services for sample extraction and QC.
Proper citation: University of Miami Sylvester Onco Genomics Shared Resource Core Facility (RRID:SCR_022502) Copy
https://www.cincinnatichildrens.org/research/divisions/b/bmi/resource-service
Provides computational resources, services, and support to investigators including clinical research informatics, application and technology development, and bioinformatics analyses.
Proper citation: Cincinnati Children's Hospital Biomedical Informatics Core Facility (RRID:SCR_022622) Copy
https://www.med.unc.edu/genomics/about/
Full service sequencing facility to assist with genetic and genomic research. Part of Integrated Genomics Cores. Offers comprehensive library services, NextGen sequencing and alternative technologies including long reads. Offers library preparations and multiple sequencing platforms including MiSeq, HiSeq, NovaSeq, NextSeq, BioNano, Oxford Nanopore. Services also include using techniques and data analyses via sister core BARC.
Proper citation: UNC School of Medicine Integrated Genomics Cores High Throughput Sequencing Core Facility (RRID:SCR_022620) Copy
https://www.cincinnatichildrens.org/research/divisions/c/critical/medialab
Develops high quality video and animation to enhance medical education.
Proper citation: Cincinnati Children's Hospital Critical Care Media Lab Core Facility (RRID:SCR_022629) Copy
Clinical Imaging Core Service Center includes RADCORE, Tumor Response Assessment (TRAC) and Research imaging guided biopsies cores. Supports cancer clinical trials. RADCORE staff include clinical research professionals with range of expertise in imaging based clinical research protocols and offers services in consultation, management of research protocols and Radiology IND/IDE support office. Tumor Response Assessment Core (TRAC) team of Radiologist is skilled in evaluating tumor response imaging assessments for patients enrolled in cancer clinical trials being conducted at UPHS. Research imaging guided biopsies Interventional Radiology and technical staff provides consultation to define biopsy protocol and pathology tissue collection.
Proper citation: University of Pennsylvania Perelman School of Medicine Clinical Imaging Service Center Core Facility (RRID:SCR_022487) Copy
https://www.cincinnatichildrens.org/research/cores/translational-core-laboratory/viral-vector-core
Provides research grade retroviral, lentiviral and adeno associated virus vectors. Offers generation of stable producer lines, and non-GMP quality control testing including vector titer by functional assay FACS or PCR, endotoxin, mycoplasma, and USP sterility testing.Provides standard packaging plasmids, the investigator provides the viral vector to be used for the production run.
Proper citation: Cincinnati Children's Hospital Viral Vector Core Facility (RRID:SCR_022641) Copy
Provides facilities and expertise to support clinical assay development from technology developed in academic laboratories.Provides assay development and testing support for early phase clinical trials with focus on cell and gene therapy.
Proper citation: Cincinnati Children's Hospital Translational Trial Development and Support Laboratory Core Facility (RRID:SCR_022640) Copy
https://sites.google.com/ucsc.edu/mal/
Operated by Institute of Marine Sciences with support from UCSC Division of Physical and Biological Sciences. Available to UCSC and University of California marine and coastal research community.Provides equipment including Shimadzu TOC-VCPH TC/TN analyzer,Dionex ICS-2000 Ion Chromatograph,UIC Carbon Coulometer Analyzer, Perkin-Elmer AAnalyst 100 Atomic Absorption Spectrophotometer, Thermo Genesys 10S UV-Visible Spectrophotometer, Turner Designs 10-AU Field Fluorometer, Turner Designs TD-700 Lab Fluorometer, Turner Designs TD-20/20 Luminometer,Lachat QuikChem 8000 Flow Injection Analyzer, Guildline 8410 PortaSal Salinometer, Labconco Freeze Dryer.
Proper citation: University of California at Santa Cruz Marine Analytical Laboratory Core Facility (RRID:SCR_022480) Copy
https://www.cincinnatichildrens.org/service/p/pathology/mass-spectrometry
Provides analytical resource focusing on application of mass spectrometry and allied chromatographies to analysis of small molecules, generally of less than 1,000 daltons molecular weight, in complex clinical and biological samples.
Proper citation: Cincinnati Children's Hospital Mass Spectrometry Core Facility (RRID:SCR_022638) Copy
https://www.unmc.edu/vcr/cores/vcr-cores/small-animal-imaging/miti/index.html
Core provides intravital imaging equipment to acquire advanced descriptive and quantitative data for imaging studies. Includes Olympus FVMPE-RS advanced multiphoton microscope, multichannel, multiphoton excitation system specifically tailored for intravital and deep tissue imaging.System is additionally equipped with 458 and 588 nm stimulation laser lines for photobleaching, light stimulation and/or photoactivation of various fluorescent or caged constructs. Uses Spectra-Physics InSight X3 dual-line, tunable multiphoton laser for ultrafast, multichannel imaging.Available Neurotar Mobile Home Cage allows researchers to integrate real-time imaging with behavioral/locomotion tracking. Offers training and access to imaging system, support in design of experimental procedures, acquisition of image data, and analysis of acquired images.
Proper citation: University of Nebraska Medical Center Multiphoton Intravital and Tissue Imaging Core Facility (RRID:SCR_022478) Copy
https://www.cincinnatichildrens.org/service/c/clinical-trials/biobank
Provides access to services for standardized and centralized acquisition, processing, storage and distribution of biospecimens for research.
Proper citation: Cincinnati Children's Hospital Discover Together Biobank Core Facility (RRID:SCR_022632) Copy
https://www.cincinnatichildrens.org/research/cores/imaging
Provides access to advanced analytical imaging and image post-processing methods for collaborators involved in basic and clinical research.
Proper citation: Cincinnati Children's Hospital Imaging Research Center Core Facility (RRID:SCR_022631) Copy
https://www.vet.upenn.edu/research/core-resources-facilities/extracellular-vesicle-core
Provides comprehensive or selected services in necessary isolation, quantification and characterization of EVs. Isolation of EV is based on size exclusion using high-performance (SEC-HPLC) or gravity fed (e.g. iZon column) liquid chromatography, ultracentrifugation, and/or density gradient ultracentrifugation. Accurately characterizes EV particle size distribution and concentration using resistive pulse sensing techniques (nCS1, Spectradyne, LLC). Immunophenotype can be accomplished using nanoscale flow cytometry and/or chip array (ExoViewTM) techniques. Provides services in training and education for individuals and lab groups in all methods above and study design consultation.
Proper citation: University of Pennsylvania Penn Vet Extracellular Vesicle Core Facility (RRID:SCR_022444) Copy
Core provides equipment for flow cytometric analysis, full service cell sorting, training, and dissemination.
Proper citation: British Columbia Children's Hospital Research Institute Flow Cytometry Core Facility (RRID:SCR_022790) Copy
https://asrc.gc.cuny.edu/neuroscience/facilities/live-imaging-bioenergetics-facility/
Provides Zeiss LSM880 upright two photon confocal microscope with Airyscan, FAST model and CLARITY lens; LSM880 inverted live cell imaging confocal microscope with Airyscan and FAST model; Agilent Seahorse XFe24 live cell metabolic analyzer.Supported applications include in vivo imaging of live animals, time lapse live cell imaging with high resolution optical sectioning, deep imaging of fixed CLARITY tissues, calcium imaging, photo switching and photo uncaging, Fluorescence Recovery After Photobleaching, Forster Resonance Energy Transfer, laser ablation, and measuring mitochondrial respiration and glycolysis in live cells in real time.Provides advanced imaging analysis software Imaris for data processing.
Proper citation: City University of New York Advanced Science Research Center Live Imaging and Bioenergetics Core Facility (RRID:SCR_022945) Copy
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