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Species: Other
Genetic Insert: TfCut2_b.45_h90_t.5_i4400_11_59
Vector Backbone Description: Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_257141 Copy
Species: Homo sapiens
Genetic Insert: Pre-thrombin 2 S525A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4396; Vector Backbone:pCMV-Sport 6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42837787
Proper citation: RRID:Addgene_255520 Copy
Species: Glycine max
Genetic Insert: 4-Coumarate-CoA ligase
Vector Backbone Description: Vector Backbone:pBbE5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42843616
Proper citation: RRID:Addgene_258003 Copy
Species: Glycine max
Genetic Insert: 4-Coumarate-CoA ligase
Vector Backbone Description: Vector Backbone:pBbE5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42843616
Proper citation: RRID:Addgene_258004 Copy
Species: Glycine max
Genetic Insert: 4-Coumarate-CoA ligase
Vector Backbone Description: Vector Backbone:pBbE5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42843616
Proper citation: RRID:Addgene_258002 Copy
Species: Glycine max
Genetic Insert: 4-Coumarate-CoA ligase
Vector Backbone Description: Vector Backbone:pBbE5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42843616
Proper citation: RRID:Addgene_258007 Copy
Species: Drosophila melanogaster
Genetic Insert: MyLOV-VI-zip
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5265; Vector Backbone:pBiEx-1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2025.08.19.666446 for bioRxiv preprint.
Proper citation: RRID:Addgene_233724 Copy
Species: Other
Genetic Insert: NT-CRISPR plasmid (pST_116) with gRNA targetting pta2
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250053 Copy
Species: E. coli BL21 (TaKaRa)
Genetic Insert: Cytosine permease
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42841632
Comments: Please note: This plasmid contains a Q419stop mutation in codA and a Q335P mutation in bpsA. These mutations are not known to impact plasmid function.
Proper citation: RRID:Addgene_246236 Copy
Species: E. coli (Ec)
Genetic Insert: AsnRS
Vector Backbone Description: Backbone Marker:Novagen / MilliporeSigma; Backbone Size:5249; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Expression is lac-inducible (IPTG).
This plasmid is an original b.next design for implementing the PURE cell-free system, first described by Shimizu et al. (Nat Biotechnol 2001, PMID 11479568). It can be produced using the OnePot method from Lavickova & Maerkl (ACS Synth Biol 2019, PMID 30632751).
Protocols for using these plasmids, along with validation data documented as Developer Notes, are linked in the Resource Information section.
For additional information about Nucleus, the open developer platform for synthetic cells, see: https://bnext.bio/nucleus.
Proper citation: RRID:Addgene_259940 Copy
Vector Backbone Description: Backbone Marker:ELEGEN; Backbone Size:2594; Vector Backbone:pAAV2-Amp; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Compatible with the Eyquem lab's SEED-Selection TCR knocking sorting technique when cloned with suitable TRBC domains (i.e. containing the D112K mutation), see https://www.nature.com/articles/s41587-024-02531-6
Compatible with BsmBI mediated makeTCR system, see https://www.biorxiv.org/content/10.1101/2025.04.27.647198v2
Compatible with Birnbaum lab's SapI mediated TCRAFT system, see https://linkinghub.elsevier.com/retrieve/pii/S1074761326000014
Replacement for previous version of vector (unreleased) that was not compatible with SEED selection sgRNA target site.
Proper citation: RRID:Addgene_256431 Copy
Species: E. coli (Ec)
Genetic Insert: GlnRS
Vector Backbone Description: Backbone Marker:Novagen / MilliporeSigma; Backbone Size:5246; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Expression is lac-inducible (IPTG).
This plasmid is an original b.next design for implementing the PURE cell-free system, first described by Shimizu et al. (Nat Biotechnol 2001, PMID 11479568). It can be produced using the OnePot method from Lavickova & Maerkl (ACS Synth Biol 2019, PMID 30632751).
Protocols for using these plasmids, along with validation data documented as Developer Notes, are linked in the Resource Information section.
For additional information about Nucleus, the open developer platform for synthetic cells, see: https://bnext.bio/nucleus.
Proper citation: RRID:Addgene_259947 Copy
Species: E. coli (Ec)
Genetic Insert: EF-Tu
Vector Backbone Description: Backbone Marker:Novagen / MilliporeSigma; Backbone Size:5246; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Expression is lac-inducible (IPTG).
This plasmid is an original b.next design for implementing the PURE cell-free system, first described by Shimizu et al. (Nat Biotechnol 2001, PMID 11479568). It can be produced using the OnePot method from Lavickova & Maerkl (ACS Synth Biol 2019, PMID 30632751).
Protocols for using these plasmids, along with validation data documented as Developer Notes, are linked in the Resource Information section.
For additional information about Nucleus, the open developer platform for synthetic cells, see: https://bnext.bio/nucleus.
Proper citation: RRID:Addgene_259946 Copy
Species: Synthetic
Genetic Insert: Estrogen Response Element
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42664973
Comments: Please visit https://doi.org/10.64898/2025.12.14.694224 for bioRxiv preprint.
Proper citation: RRID:Addgene_254810 Copy
Species: E. coli (Ec)
Genetic Insert: EF-Ts
Vector Backbone Description: Backbone Marker:Novagen / MilliporeSigma; Backbone Size:5246; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Expression is lac-inducible (IPTG).
This plasmid is an original b.next design for implementing the PURE cell-free system, first described by Shimizu et al. (Nat Biotechnol 2001, PMID 11479568). It can be produced using the OnePot method from Lavickova & Maerkl (ACS Synth Biol 2019, PMID 30632751).
Protocols for using these plasmids, along with validation data documented as Developer Notes, are linked in the Resource Information section.
For additional information about Nucleus, the open developer platform for synthetic cells, see: https://bnext.bio/nucleus.
Proper citation: RRID:Addgene_259945 Copy
Species: Other
Genetic Insert: dropout part, lv0, pos 3, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249993 Copy
Species: Other
Genetic Insert: dropout part, lv0, pos 4, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249995 Copy
Species: Other
Genetic Insert: dropout part, lv0, pos 2, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249991 Copy
Species: Homo sapiens
Genetic Insert: PPP2R1A
Vector Backbone Description: Backbone Marker:Dr. Christian J. Braun; Backbone Size:9425; Vector Backbone:Cas13_DR1_dsRed-T2A-Puro; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin and Bleocin (Zeocin)
Defining Citation: PMID:42266180
Proper citation: RRID:Addgene_248705 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike
Vector Backbone Description: Vector Backbone:pDMJ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37034621
Comments: Please visit https://doi.org/10.1101/2023.03.30.535005 for bioRxiv preprint.
Proper citation: RRID:Addgene_257098 Copy
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