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Plasmids are provided by Addgene and DGRC.

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https://www.addgene.org/pooled-library/bloom-sars-cov-2-rbd-ssm/

Vector Backbone Description: Vector Types:Yeast Expression
Comments: 420_pETcon_SARS2_LP.8.1.1 (Addgene #254411), SARS-CoV-2 RBD LP.8.1.1 SSM library (lib1) (Addgene #254414), SARS-CoV-2 RBD LP.8.1.1 SSM library (lib2) (Addgene #254415)

Proper citation: RRID:Addgene_1000000281 Copy   


https://www.addgene.org/kits/doelsnitz-ired-screening/

Vector Backbone Description: Vector Types:Bacterial Expression, Synthetic Biology
Comments: pIR01-AUD39529.1 (Addgene #232673), pIR02-AUD39583.1 (Addgene #232674), pIR03-EMF01349.1 (Addgene #232675), pIR04-NUT93668.1 (Addgene #232676), pIR05-WP_009080771.1 (Addgene #232677), pIR06-WP_013019548.1 (Addgene #232678), pIR07-WP_083466898.1 (Addgene #232679), pIR08-WP_123102485.1 (Addgene #232680), pIR09-WP_123993384.1 (Addgene #232681), pIR10-WP_132480478.1 (Addgene #232682), pIR11-WP_232847731.1 (Addgene #232683), pIR12-WP_240033132.1 (Addgene #232684), pIR13-WP_253870842.1 (Addgene #232685), pIR14-WP_277212655.1 (Addgene #232686), pIR15-MF540792.1 (Addgene #232687), pIR16-AUD39487.1 (Addgene #232688), pIR17-ATL31837.1 (Addgene #232689), pIR18-ATW48861.1 (Addgene #232690), pIR19-AUD39517.1 (Addgene #232691), pIR20-EME99395.1 (Addgene #232692), pIR21-OMI34305.1 (Addgene #232693), pIR22-PRX45020.1 (Addgene #232694), pIR23-WP_030629722.1 (Addgene #232695), pIR24-WP_037254083.1 (Addgene #232696), pIR25-WP_111548309.1 (Addgene #232697), pIR26-WP_112256886.1 (Addgene #232698), pIR27-WP_116022473.1 (Addgene #232699), pIR28-WP_220260546.1 (Addgene #232700), pIR29-WP_230421476.1 (Addgene #232701), pIR30-WP_240598438.1 (Addgene #232702), pIR31-GHE75528.1 (Addgene #232703), pIR32-MCP2166807.1 (Addgene #232704), pIR33-PFG99428.1 (Addgene #232705), pIR34-WP_029384055.1 (Addgene #232706), pIR35-WP_073484372.1 (Addgene #232707), pIR36-WP_073486480.1 (Addgene #232708), pIR37-WP_084468547.1 (Addgene #232709), pIR38-WP_172381658.1 (Addgene #232710), pIR39-WP_184965005.1 (Addgene #232711), pIR40-WP_187234818.1 (Addgene #232712), pIR41-WP_189878949.1 (Addgene #232713), pIR42-WP_191257941.1 (Addgene #232714), pIR43-WP_229347914.1 (Addgene #232715), pIR44-WP_242906400.1 (Addgene #232716), pIR45-WP_253669224.1 (Addgene #232717), pIR46-WP_266533875.1 (Addgene #232718), pScreen-R2.1 (Addgene #232672), pScreen-S1.3 (Addgene #232671)

Proper citation: RRID:Addgene_1000000260 Copy   


https://www.addgene.org/kits/youles-moclo-plant/

Vector Backbone Description: Vector Types:Synthetic Biology
Comments: pICSL01002_CP (Addgene #245687), pICSL01003_CP (Addgene #245688), pICSL01005-LC (Addgene #245697), pICSL01005_CP (Addgene #245689), pICSL01008_CP (Addgene #245690), pICSL12001 (Addgene #245625), pICSL12002 (Addgene #245626), pICSL12005 (Addgene #245627), pICSL12007 (Addgene #245628), pICSL12009A (Addgene #245629), pICSL12013 (Addgene #245630), pICSL12014 (Addgene #245631), pICSL12015 (Addgene #245632), pICSL12026 (Addgene #245633), pICSL12027 (Addgene #245634), pICSL12028 (Addgene #245635), pICSL12029 (Addgene #245636), pICSL12030 (Addgene #245637), pICSL12033 (Addgene #245638), pICSL12034 (Addgene #245639), pICSL12038 (Addgene #245640), pICSL12039 (Addgene #245641), pICSL12040 (Addgene #245642), pICSL12046 (Addgene #245643), pICSL12047 (Addgene #245644), pICSL12048 (Addgene #245645), pICSL12049 (Addgene #245646), pICSL12050 (Addgene #245647), pICSL12055 (Addgene #245648), pICSL12063 (Addgene #245649), pICSL12080 (Addgene #245650), pICSL12081 (Addgene #245651), pICSL12082 (Addgene #245652), pICSL13003 (Addgene #245653), pICSL13004 (Addgene #245654), pICSL13005 (Addgene #245655), pICSL13008 (Addgene #245656), pICSL13009 (Addgene #245657), pICSL13010 (Addgene #245658), pICSL13015 (Addgene #245659), pICSL13016A (Addgene #245660), pICSL13017 (Addgene #245661), pICSL13018 (Addgene #245662), pICSL13019 (Addgene #245663), pICSL13020 (Addgene #245664), pICSL41258_CP (Addgene #245691), pICSL41264_CP (Addgene #245692), pICSL41276_CP (Addgene #245693), pICSL41295_CP (Addgene #245694), pICSL41308-LC (Addgene #245696), pICSL41308_CP (Addgene #245695), pICSL47732-LC (Addgene #245712), pICSL47732_RFP (Addgene #245698), pICSL47742-LC (Addgene #245713), pICSL47742_RFP (Addgene #245699), pICSL47751-LC (Addgene #245714), pICSL47751_RFP (Addgene #245700), pICSL47761-LC (Addgene #245715), pICSL47761_RFP (Addgene #245701), pICSL47772-LC (Addgene #245716), pICSL47772_RFP (Addgene #245702), pICSL47781-LC (Addgene #245717), pICSL47781_RFP (Addgene #245703), pICSL47791-LC (Addgene #245718), pICSL47791_RFP (Addgene #245704), pICSL47799 (Addgene #245719), pICSL47802_RFP (Addgene #245705), pICSL47811_RFP (Addgene #245706), pICSL47822_RFP (Addgene #245707), pICSL47831_RFP (Addgene #245708), pICSL47841_RFP (Addgene #245709), pICSL47852_RFP (Addgene #245710), pICSL47861_RFP (Addgene #245711), pICSL47869 (Addgene #245720), pICSL60002 (Addgene #245665), pICSL60005 (Addgene #245666), pICSL60006 (Addgene #245667), pICSL60007 (Addgene #245668), pICSL60008 (Addgene #245669), pICSL60013 (Addgene #245670), pICSL60020 (Addgene #245671), pICSL60024 (Addgene #245672), pICSL60025 (Addgene #245673), pICSL60026 (Addgene #245674), pICSL60027 (Addgene #245675), pICSL60028 (Addgene #245676), pICSL60038 (Addgene #245677), pICSL61001 (Addgene #245678), pICSL61002 (Addgene #245679), pICSL61003 (Addgene #245680), pICSL61004 (Addgene #245681), pICSL62001 (Addgene #245682), pICSL62002 (Addgene #245683), pICSL62003 (Addgene #245684), pICSL62004 (Addgene #245685), pICSL62005 (Addgene #245686)

Proper citation: RRID:Addgene_1000000278 Copy   


https://www.addgene.org/kits/youles-moclo-plant/

Vector Backbone Description: Vector Types:Synthetic Biology
Comments: pICSL30001 (Addgene #245529), pICSL30002 (Addgene #245530), pICSL30007 (Addgene #245531), pICSL30012 (Addgene #245532), pICSL30013 (Addgene #245533), pICSL30014 (Addgene #245534), pICSL30016A (Addgene #245535), pICSL30020 (Addgene #245536), pICSL30021 (Addgene #245537), pICSL30023 (Addgene #245538), pICSL30024 (Addgene #245539), pICSL30026 (Addgene #245540), pICSL30031 (Addgene #245541), pICSL30032 (Addgene #245542), pICSL30033 (Addgene #245543), pICSL30034 (Addgene #245544), pICSL30040 (Addgene #245545), pICSL30041 (Addgene #245546), pICSL30042 (Addgene #245547), pICSL30043 (Addgene #245548), pICSL30044 (Addgene #245549), pICSL30047 (Addgene #245550), pICSL30048 (Addgene #245551), pICSL30049 (Addgene #245552), pICSL30050 (Addgene #245553), pICSL30051 (Addgene #245554), pICSL30052 (Addgene #245555), pICSL30053 (Addgene #245556), pICSL30054 (Addgene #245557), pICSL30055 (Addgene #245558), pICSL30056 (Addgene #245559), pICSL30057 (Addgene #245560), pICSL30058 (Addgene #245561), pICSL30059 (Addgene #245562), pICSL30060 (Addgene #245563), pICSL30061 (Addgene #245564), pICSL30062 (Addgene #245565), pICSL30063 (Addgene #245566), pICSL30064 (Addgene #245567), pICSL30065 (Addgene #245568), pICSL30066 (Addgene #245569), pICSL30068 (Addgene #245570), pICSL30069 (Addgene #245571), pICSL30070 (Addgene #245572), pICSL30071 (Addgene #245573), pICSL30072 (Addgene #245574), pICSL30073 (Addgene #245575), pICSL50002 (Addgene #245576), pICSL50003 (Addgene #245577), pICSL50005 (Addgene #245578), pICSL50017 (Addgene #245579), pICSL50018 (Addgene #245580), pICSL50019 (Addgene #245581), pICSL50020 (Addgene #245582), pICSL50021 (Addgene #245583), pICSL50022 (Addgene #245584), pICSL50023 (Addgene #245585), pICSL50028 (Addgene #245586), pICSL50029 (Addgene #245587), pICSL50030 (Addgene #245588), pICSL50031 (Addgene #245589), pICSL50032 (Addgene #245590), pICSL50033 (Addgene #245591), pICSL50034 (Addgene #245592), pICSL50035 (Addgene #245593), pICSL50036 (Addgene #245594), pICSL50037 (Addgene #245595), pICSL50038 (Addgene #245596), pICSL50039 (Addgene #245597), pICSL50040 (Addgene #245598), pICSL50041 (Addgene #245599), pICSL50042 (Addgene #245600), pICSL50043 (Addgene #245601), pICSL50044 (Addgene #245602), pICSL50047 (Addgene #245603), pICSL50048 (Addgene #245604), pICSL50049 (Addgene #245605), pICSL50050 (Addgene #245606), pICSL50051 (Addgene #245607), pICSL50052 (Addgene #245608), pICSL50053 (Addgene #245609), pICSL50055 (Addgene #245610), pICSL50056 (Addgene #245611), pICSL50057 (Addgene #245612), pICSL50058 (Addgene #245613), pICSL50059 (Addgene #245614), pICSL50060 (Addgene #245615), pICSL50061 (Addgene #245616), pICSL50062 (Addgene #245617), pICSL50063 (Addgene #245618), pICSL50064 (Addgene #245619), pICSL50065 (Addgene #245620), pICSL50066 (Addgene #245621), pICSL50067 (Addgene #245622), pICSL50068 (Addgene #245623), pICSL50069 (Addgene #245624)

Proper citation: RRID:Addgene_1000000277 Copy   


http://www.addgene.org/29589

Species: H. sapiens (human)
Genetic Insert: pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro (Addgene ##28289)
Vector Backbone Description: Vector Types: Cell Surface, Extracellular, and DNA Binding Targets; Bacterial Resistance: Ampicillin;

Proper citation: RRID:Addgene_29589 Copy   


http://www.addgene.org/28288

Species: M. musculus (mouse)
Genetic Insert: pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro (Addgene ##28289)
Vector Backbone Description: Vector Types: Disease Targets; Bacterial Resistance: Ampicillin;

Proper citation: RRID:Addgene_28288 Copy   


http://www.addgene.org/28286

Species: H. sapiens (human)
Genetic Insert: pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro (Addgene ##28289)
Vector Backbone Description: Vector Types: Disease Targets; Bacterial Resistance: Ampicillin;

Proper citation: RRID:Addgene_28286 Copy   


http://www.addgene.org/28287

Species: M. musculus (mouse)
Genetic Insert: pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro (Addgene ##28289)
Vector Backbone Description: Vector Types: Pathway Targets; Bacterial Resistance: Ampicillin;

Proper citation: RRID:Addgene_28287 Copy   


http://www.addgene.org/255881

Species: Synthetic
Genetic Insert: NovoSplit657(A)
Vector Backbone Description: Backbone Size:5360; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42461986
Comments: Please visit https://doi.org/10.1101/2025.08.03.668343 for bioRxiv preprint.

Proper citation: RRID:Addgene_255881 Copy   


http://www.addgene.org/255882

Genetic Insert: NovoSplit657(B)
Vector Backbone Description: Backbone Size:5606; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42461986
Comments: Please visit https://doi.org/10.1101/2025.08.03.668343 for bioRxiv preprint.

Proper citation: RRID:Addgene_255882 Copy   


  • RRID:Addgene_253620

http://www.addgene.org/253620

Genetic Insert: FLP recombinase
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Comments: Alternative name: pRC453 Usage/Comments: This plasmid is used to generate a cSAGE-compatible base strain. Once pSC01 is integrated into the chromosome, this plasmid expresses FLP recombinase to cure / excise the backbone marker and contains a SacB marker for sucrose counterselection. This plasmid is used only for installation of the landing pad and is not required for downstream integration steps after the base strain is constructed. Please visit https://doi.org/10.64898/2026.04.17.717921 for bioRxiv preprint.

Proper citation: RRID:Addgene_253620 Copy   


  • RRID:Addgene_254712

http://www.addgene.org/254712

Species: Saccharomyces cerevisiae
Genetic Insert: YKU70 sgRNA
Vector Backbone Description: Backbone Marker:John Wyrick lab; Backbone Size:11240; Vector Backbone:pML104; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:42519825
Comments: This plasmid contains an example guide sequence targeting **YKU70**. As described in our protocol paper, the guide sequence can be easily changed by designing forward and reverse primers for a new target and then using PCR and seamless plasmid recircularization.

Proper citation: RRID:Addgene_254712 Copy   


  • RRID:Addgene_250521

http://www.addgene.org/250521

Species: Synthetic
Genetic Insert: FenixS
Vector Backbone Description: Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.1101/2025.11.29.691301 for bioRxiv preprint.

Proper citation: RRID:Addgene_250521 Copy   


  • RRID:Addgene_250520

http://www.addgene.org/250520

Species: Synthetic
Genetic Insert: FenixS
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2025.11.29.691301 for bioRxiv preprint.

Proper citation: RRID:Addgene_250520 Copy   


http://www.addgene.org/257837

Species: Salmonella enterica serovar Typhimurium
Genetic Insert: PssaG-BBa_B0034-sfGFP-LVA-L3S2P21
Vector Backbone Description: Backbone Marker:modified from EcoFlex library; Backbone Size:3751; Vector Backbone:pTU2-a-pSC101-Kan; Vector Types:Bacterial Expression, Golden Gate (EcoFlex); Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_257837 Copy   


  • RRID:Addgene_256749

http://www.addgene.org/256749

Vector Backbone Description: Vector Backbone:pSFS2A; Vector Types:Synthetic Biology, Candida albicans expression; Bacterial Resistance:Chloramphenicol
Comments: Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. The different orientation of the F1 ori does not alter the plasmid's function. Please refer to the the Addgene sequence for the most accurate result. Please visit https://doi.org/10.64898/2026.05.11.723596 for bioRxiv preprint.

Proper citation: RRID:Addgene_256749 Copy   


  • RRID:Addgene_256742

http://www.addgene.org/256742

Vector Backbone Description: Vector Backbone:pSFS2A; Vector Types:Synthetic Biology, Candida albicans expression; Bacterial Resistance:Chloramphenicol
Comments: Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. The different orientation of the F1 ori does not alter the plasmid's function. Please refer to the the Addgene sequence for the most accurate result. Please visit https://doi.org/10.64898/2026.05.11.723596 for bioRxiv preprint.

Proper citation: RRID:Addgene_256742 Copy   


  • RRID:Addgene_256743

http://www.addgene.org/256743

Vector Backbone Description: Vector Backbone:pSFS2A; Vector Types:Synthetic Biology, Candida albicans expression; Bacterial Resistance:Chloramphenicol
Comments: Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. The different orientation of the F1 ori does not alter the plasmid's function. Please refer to the the Addgene sequence for the most accurate result. Please visit https://doi.org/10.64898/2026.05.11.723596 for bioRxiv preprint.

Proper citation: RRID:Addgene_256743 Copy   


http://www.addgene.org/255258

Species: Homo sapiens
Genetic Insert: Mito
Vector Backbone Description: Backbone Marker:Takara Bio; Vector Backbone:pIVT-BspQI; Vector Types:In-vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42310021

Proper citation: RRID:Addgene_255258 Copy   


  • RRID:Addgene_255257

http://www.addgene.org/255257

Species: Synthetic
Genetic Insert: ERmoxGFP
Vector Backbone Description: Backbone Marker:Takara Bio; Vector Backbone:pIVT-BspQI; Vector Types:In-vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42310021

Proper citation: RRID:Addgene_255257 Copy   



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