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Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.B2 VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26829320
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_103003 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.B VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26829320
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_103002 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.eB VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_103005 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.B3 VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26829320
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_103004 Copy
Species: Synthetic
Genetic Insert: TIMER-bac
Vector Backbone Description: Backbone Size:9262; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25126781
Comments: TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance.
Proper citation: RRID:Addgene_103057 Copy
Species: Synthetic
Genetic Insert: YFP-SspBx6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29115293
Proper citation: RRID:Addgene_103778 Copy
Species: Synthetic
Genetic Insert: TIA1 RRM-mCherry-iLIDx6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4717; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29115293
Proper citation: RRID:Addgene_103782 Copy
Species: Synthetic
Genetic Insert: TIA1 RRM-CFP-FRBx5
Vector Backbone Description: Backbone Marker:Unknown; Backbone Size:5100; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29115293
Proper citation: RRID:Addgene_103783 Copy
Species: synthetic
Genetic Insert: CAS9-MSA
Vector Backbone Description: Backbone Size:4072; Vector Backbone:PCS2+; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Comments: non
Proper citation: RRID:Addgene_103882 Copy
Species: Synthetic
Genetic Insert: DsRed-Express2
Vector Backbone Description: Backbone Size:3224; Vector Backbone:pEB2; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This FP derives from DsRed. Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_103999 Copy
Species: Synthetic
Genetic Insert: mClover3
Vector Backbone Description: Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This FP derives from wtGFP. In this plasmid, the FP is coded with the original wtGFP nucleotide coding sequence except where mutations specific to the FP occur (see map). Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_103994 Copy
Species: Synthetic
Genetic Insert: 2ndVal DsRed-Express2
Vector Backbone Description: Backbone Size:3224; Vector Backbone:pEB2; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This is a version of DsRed-Express2 with a valine inserted in the second position. Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_104024 Copy
Species: Synthetic
Genetic Insert: comClover3
Vector Backbone Description: Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This is a codon optimized version of mClover3. Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_104023 Copy
Species: Synthetic
Genetic Insert: 2ndVal mCherry-L
Vector Backbone Description: Backbone Size:3224; Vector Backbone:pEB2; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This is a version of mCherry-L with a valine inserted in the second position. Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_104026 Copy
Species: Synthetic
Genetic Insert: comVenJBC
Vector Backbone Description: Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This is a codon optimized version of mVenus JBC. Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_104020 Copy
Species: Synthetic
Genetic Insert: coClover
Vector Backbone Description: Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This is a codon optimized version of Clover. Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_104022 Copy
Species: Synthetic
Genetic Insert: comVenME
Vector Backbone Description: Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This is a codon optimized version of mVenus ME. Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_104021 Copy
Species: Synthetic
Genetic Insert: T-Sapphire
Vector Backbone Description: Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This FP derives from wtGFP. In this plasmid, the FP is coded with the original wtGFP nucleotide coding sequence except where mutations specific to the FP occur (see map). Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_103977 Copy
Species: Synthetic
Genetic Insert: mCerulean3
Vector Backbone Description: Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This FP derives from wtGFP. In this plasmid, the FP is coded with the original wtGFP nucleotide coding sequence except where mutations specific to the FP occur (see map). Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_103973 Copy
Species: Synthetic
Genetic Insert: 2ndVal mScarlet-I
Vector Backbone Description: Backbone Size:3224; Vector Backbone:pEB2; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29320486
Comments: This is a version of mScarlet-I with a valine inserted in the second position. Please see "Notes On Plasmids In This Collection" document for further details and references.
Proper citation: RRID:Addgene_104028 Copy
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