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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pUCmini-iCAP-PHP.B2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103003 | Synthetic construct isolate AAV-PHP.B2 VP1 gene | Synthetic | Ampicillin | PMID:26829320 | Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. | Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 1 | |
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pUCmini-iCAP-PHP.B Resource Report Resource Website 10+ mentions |
RRID:Addgene_103002 | Synthetic construct isolate AAV-PHP.B VP1 gene | Synthetic | Ampicillin | PMID:26829320 | Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. | Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 14 | |
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pUCmini-iCAP-PHP.eB Resource Report Resource Website 100+ mentions |
RRID:Addgene_103005 | Synthetic construct isolate AAV-PHP.eB VP1 gene | Synthetic | Ampicillin | PMID:28671695 | Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. | Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 135 | |
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pUCmini-iCAP-PHP.B3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103004 | Synthetic construct isolate AAV-PHP.B3 VP1 gene | Synthetic | Ampicillin | PMID:26829320 | Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. | Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 1 | |
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pSC101_TIMER Resource Report Resource Website 1+ mentions |
RRID:Addgene_103057 | TIMER-bac | Synthetic | Kanamycin | PMID:25126781 | TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance. | Backbone Size:9262; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | mutations: S4T and S179T | 2026-09-12 02:13:54 | 4 |
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YFP-SspBx6 Resource Report Resource Website |
RRID:Addgene_103778 | YFP-SspBx6 | Synthetic | Kanamycin | PMID:29115293 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-12 02:14:02 | 0 | ||
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TIA1 RRM-mCherry-iLIDx6 Resource Report Resource Website |
RRID:Addgene_103782 | TIA1 RRM-mCherry-iLIDx6 | Synthetic | Kanamycin | PMID:29115293 | Backbone Marker:Clontech; Backbone Size:4717; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-12 02:14:02 | 0 | ||
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pMT2 TIA1 RRM-CFP-FRBx5 Resource Report Resource Website |
RRID:Addgene_103783 | TIA1 RRM-CFP-FRBx5 | Synthetic | Ampicillin | PMID:29115293 | Backbone Marker:Unknown; Backbone Size:5100; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:14:02 | 0 | ||
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PCS2+Cas9-mSA Resource Report Resource Website 1+ mentions |
RRID:Addgene_103882 | CAS9-MSA | synthetic | Ampicillin | PMID:29889212 | non | Backbone Size:4072; Vector Backbone:PCS2+; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | none | 2026-09-12 02:14:03 | 9 |
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pEB2-DsRed-Express2 Resource Report Resource Website |
RRID:Addgene_103999 | DsRed-Express2 | Synthetic | Kanamycin | PMID:29320486 | This FP derives from DsRed. Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3224; Vector Backbone:pEB2; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | Mutations relative to DsRed (R2D, S4T, K5E, E10P, R17H, T21S, R36K, H41T, N42Q, V44A, K47Q, C117T, K121H, M141L, A145P, D169G, Q188K, I210V, T217A, G219A, L225Q) | 2026-09-12 02:14:04 | 0 |
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pEB1-mClover3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103994 | mClover3 | Synthetic | Kanamycin | PMID:29320486 | This FP derives from wtGFP. In this plasmid, the FP is coded with the original wtGFP nucleotide coding sequence except where mutations specific to the FP occur (see map). Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | Mutations relative to wild-type GFP (S30R, Y39N, S65G, Q69A, F99S, N105T, Y145F, N149Y, M153T, G160C, V163A, I171V, T203H, A206K) | 2026-09-12 02:14:04 | 1 |
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pEB2-2ndVal DsRedEx2 Resource Report Resource Website |
RRID:Addgene_104024 | 2ndVal DsRed-Express2 | Synthetic | Kanamycin | PMID:29320486 | This is a version of DsRed-Express2 with a valine inserted in the second position. Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3224; Vector Backbone:pEB2; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | DsRed-Express2 with valine inserted in 2nd position | 2026-09-12 02:14:05 | 0 |
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pEB1-comClover3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_104023 | comClover3 | Synthetic | Kanamycin | PMID:29320486 | This is a codon optimized version of mClover3. Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | Same protein sequence as mClover3 (codon optimized nucleotide sequence) | 2026-09-12 02:14:05 | 1 |
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pEB2-2ndVal mCherry-L Resource Report Resource Website |
RRID:Addgene_104026 | 2ndVal mCherry-L | Synthetic | Kanamycin | PMID:29320486 | This is a version of mCherry-L with a valine inserted in the second position. Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3224; Vector Backbone:pEB2; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | mCherry-L with valine inserted in 2nd position | 2026-09-12 02:14:05 | 0 |
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pEB1-comVenJBC Resource Report Resource Website 1+ mentions |
RRID:Addgene_104020 | comVenJBC | Synthetic | Kanamycin | PMID:29320486 | This is a codon optimized version of mVenus JBC. Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | Same protein sequence as mVenus JBC (codon optimized nucleotide sequence) | 2026-09-12 02:14:05 | 1 |
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pEB1-coClover Resource Report Resource Website 1+ mentions |
RRID:Addgene_104022 | coClover | Synthetic | Kanamycin | PMID:29320486 | This is a codon optimized version of Clover. Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | Same protein sequence as Clover (codon optimized nucleotide sequence) | 2026-09-12 02:14:05 | 1 |
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pEB1-comVenME Resource Report Resource Website 1+ mentions |
RRID:Addgene_104021 | comVenME | Synthetic | Kanamycin | PMID:29320486 | This is a codon optimized version of mVenus ME. Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | Same protein sequence as mVenus ME (codon optimized nucleotide sequence) | 2026-09-12 02:14:05 | 1 |
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pEB1-T-Sapphire Resource Report Resource Website 1+ mentions |
RRID:Addgene_103977 | T-Sapphire | Synthetic | Kanamycin | PMID:29320486 | This FP derives from wtGFP. In this plasmid, the FP is coded with the original wtGFP nucleotide coding sequence except where mutations specific to the FP occur (see map). Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | Mutations relative to wild-type GFP (Q69M, C70V, S72A, Y145F, V163A, S175G, T203I) | 2026-09-12 02:14:04 | 4 |
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pEB1-mCerulean3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103973 | mCerulean3 | Synthetic | Kanamycin | PMID:29320486 | This FP derives from wtGFP. In this plasmid, the FP is coded with the original wtGFP nucleotide coding sequence except where mutations specific to the FP occur (see map). Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3674; Vector Backbone:pEB1; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | Mutations relative to wild-type GFP (F64L, Y66W, S72A, Y145A, N146I, S147H, H148G, M153T, V163A, K166G, I167L, R168N, H169C, A206K) | 2026-09-12 02:14:04 | 2 |
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pEB2-2ndVal mScarlet-I Resource Report Resource Website |
RRID:Addgene_104028 | 2ndVal mScarlet-I | Synthetic | Kanamycin | PMID:29320486 | This is a version of mScarlet-I with a valine inserted in the second position. Please see "Notes On Plasmids In This Collection" document for further details and references. | Backbone Size:3224; Vector Backbone:pEB2; Vector Types:Bacterial Expression, Low copy; Bacterial Resistance:Kanamycin | mScarlet-I with valine inserted in 2nd position | 2026-09-12 02:14:05 | 0 |
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