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Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: In our experience, plasmid yields are highest when cells are harvested at late log stage.
Proper citation: RRID:Addgene_30501 Copy
Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: In our experience, plasmid yields are highest when cells are harvest at late log stage.
Proper citation: RRID:Addgene_30503 Copy
Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: In our experience, plasmid yields are highest when cells are harvested at late log stage.
Proper citation: RRID:Addgene_30502 Copy
Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: In our experience, plasmid yields are highest when cells are harvest at late log stage.
Note that this plasmid also confers resistance to Spectinomycin, but Addgene provides it in an Amp stab.
Proper citation: RRID:Addgene_30505 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphate kinase
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38333 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphate kinase
Vector Backbone Description: Backbone Size:5389; Vector Backbone:pETM-41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_38334 Copy
Species: Escherichia coli
Genetic Insert: Lon protease fragment
Vector Backbone Description: Vector Backbone:pETM41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: See "Nomura, K., Kato, J., Takiguchi, N., Ohtake, H., Kuroda, A., 2004. Effects of inorganic polyphosphate on the proteolytic and DNA-binding activities of Lon in Escherichia coli. J. Biol. Chem. 279, 34406–34410." for more information.
Proper citation: RRID:Addgene_38330 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphatase
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: See "Bolesch, D.G., Keasling, J.D., 2000. Polyphosphate binding and chain length recognition of Escherichia coli exopolyphosphatase. J. Biol. Chem. 275, 33814–33819" for more information.
Proper citation: RRID:Addgene_38328 Copy
Species: Escherichia coli
Genetic Insert: rpoA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3993; Vector Backbone:pGEMEX1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45398 Copy
Species: Escherichia coli
Genetic Insert: LacI
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4092; Vector Backbone:pBADmyc-hisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: LacI expression was induced with 0.2% (w/v) L-arabinose and cells incubated for 4 h at 20 °C.
Proper citation: RRID:Addgene_46394 Copy
Species: Escherichia coli
Genetic Insert: Ec86 RT
Vector Backbone Description: Backbone Marker:Fraser lab; Vector Backbone:pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170185 Copy
Species: Escherichia coli
Genetic Insert: Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Vector Backbone Description: Backbone Size:3907; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Introduced in Reynolds et al. Cell 2011, PMID: 22196731.
Proper citation: RRID:Addgene_171954 Copy
Species: Escherichia coli
Genetic Insert: Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Vector Backbone Description: Backbone Size:5206; Vector Backbone:phis8-3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Designed and introduced as A120-NoJ in PMID: 18927392, Further described as DL121 in PMID: 22196731
Proper citation: RRID:Addgene_171953 Copy
Species: Escherichia coli
Genetic Insert: B7N6P4
Vector Backbone Description: Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert name is a UniProt identifier
Proper citation: RRID:Addgene_163180 Copy
Species: Escherichia coli
Genetic Insert: TcR (tetA/tetR)
Vector Backbone Description: Backbone Size:2677; Vector Backbone:pMK2010; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: The plasmids from this article are also used with plasmid pXint129 (www.addgene.org/178208).
Proper citation: RRID:Addgene_178086 Copy
Species: Escherichia coli
Genetic Insert: lacI
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_167838 Copy
Species: Escherichia coli
Genetic Insert: lacI
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_167837 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167966 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167964 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc with Y705E, Y706E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167955 Copy
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