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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pUCmini-iCAP-PHP.B2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103003 | Synthetic construct isolate AAV-PHP.B2 VP1 gene | Synthetic | Ampicillin | PMID:26829320 | Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. | Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 1 | |
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pUCmini-iCAP-PHP.B Resource Report Resource Website 10+ mentions |
RRID:Addgene_103002 | Synthetic construct isolate AAV-PHP.B VP1 gene | Synthetic | Ampicillin | PMID:26829320 | Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. | Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 14 | |
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pUCmini-iCAP-PHP.eB Resource Report Resource Website 100+ mentions |
RRID:Addgene_103005 | Synthetic construct isolate AAV-PHP.eB VP1 gene | Synthetic | Ampicillin | PMID:28671695 | Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. | Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 135 | |
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pUCmini-iCAP-PHP.B3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103004 | Synthetic construct isolate AAV-PHP.B3 VP1 gene | Synthetic | Ampicillin | PMID:26829320 | Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. | Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 1 | |
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pCAGGS_3E5E_luciferase Resource Report Resource Website 1+ mentions |
RRID:Addgene_103055 | Ebolavirus minigenome | Zaire ebolavirus, Mayinga variant | Ampicillin | PMID:28807685 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 2 | ||
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pSC101_TIMER Resource Report Resource Website 1+ mentions |
RRID:Addgene_103057 | TIMER-bac | Synthetic | Kanamycin | PMID:25126781 | TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance. | Backbone Size:9262; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | mutations: S4T and S179T | 2026-09-12 02:13:54 | 4 |
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pCAGGS_L_EBOV Resource Report Resource Website 1+ mentions |
RRID:Addgene_103052 | L gene of Ebola virus | Zaire ebolavirus, Mayinga | Ampicillin | PMID:28807685 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 4 | ||
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pCAGGS_VP30_EBOV Resource Report Resource Website 1+ mentions |
RRID:Addgene_103051 | VP30 gene of Ebola virus | Zaire ebolavirus, Mayinga | Ampicillin | PMID:28807685 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 3 | ||
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pCAGGS_HA-VP35_EBOV Resource Report Resource Website 1+ mentions |
RRID:Addgene_103053 | VP35 | Zaire ebolavirus, Mayinga | Ampicillin | PMID:27252530 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 1 | ||
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pKSJ331 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103063 | Colicin E1 and E1 immunity protein | E. coli | Ampicillin | PMID:19029376 | Insert was cloned from ~base pair 5015 to ~500 of the circular ColE1 plasmid, where numbering begins within the colicin E1 gene. The ColE1 sequences include both the promoter for colicin E1 and for E1 immunity protein, which is transcribed in the opposite direction from the colicin gene. The cloned DNA does not include the intact kil (lysis protein) gene. | Backbone Marker:NEB; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 1 | |
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pLenti-Crispr-V2-HMID.v1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103062 | Cas9 plus expressed GESTALT V1-V5 guide | Homo sapiens | Ampicillin | PMID:27229144 | Derived from https://www.addgene.org/52961/ | Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 4 | |
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pAAV-CMV-FLEX-TVAmCherry-2A-oG Resource Report Resource Website 1+ mentions |
RRID:Addgene_102985 | TVA-mCherry fusion protein after CRE-mediated recombination | Ampicillin | PMID:28689641 | Vector Backbone:pAAV; Vector Types:AAV, Adeno Associated Viral Vector; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 1 | |||
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pCAGGS_VP35_EBOV Resource Report Resource Website 1+ mentions |
RRID:Addgene_103050 | VP35 gene of Ebola virus | (Zaire ebolavirus, Mayinga) | Ampicillin | PMID:28807685 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 4 | ||
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STAgR_Neo Resource Report Resource Website 1+ mentions |
RRID:Addgene_102992 | Ampicillin | PMID:29702666 | Backbone Marker:Stricker Lab; Vector Backbone:Human gRNA Expression Vector / PCR Template for STAgR; Vector Types:CRISPR, PCR Template for STAgR Vectors; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 1 | ||||
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pLKO.1P shNFS1_2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_102964 | shNFS1_2 | Homo sapiens | Ampicillin | PMID:29168506 | TRCN0000229755 | Backbone Marker:Broad Institute; Backbone Size:7466; Vector Backbone:pLKO_TRC005; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:53 | 2 | |
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pLKO.1P shNFS1_4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_102966 | shNFS1_4 | Homo sapiens | Ampicillin | PMID:29168506 | TRCN0000180881 | Backbone Marker:Broad Institute; Backbone Size:7466; Vector Backbone:pLKO.1P; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:53 | 1 | |
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pUDE724 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103023 | crPDR12-3.S | Saccharomyces cerevisiae | Ampicillin | PMID:29106617 | Backbone Marker:Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR; Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 1 | ||
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pUDE735 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103024 | crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S | Saccharomyces cerevisiae | Ampicillin | PMID:29106617 | Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 2 | ||
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pUDE714 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103021 | crHIS4-4.S | Saccharomyces cerevisiae | Ampicillin | PMID:29106617 | Backbone Marker:Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR; Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 1 | ||
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pUDE710 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103020 | crADE2-3.crHIS4-4.S | Saccharomyces cerevisiae | Ampicillin | PMID:29106617 | Backbone Marker:Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR; Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-12 02:13:54 | 2 |
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