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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pMSP3535 Resource Report Resource Website 1+ mentions |
RRID:Addgene_46886 | Erythromycin | PMID:10964628 | This plasmid utilizes the NIsin Controlled Expression (NICE) system. NICE® is a registered trademark of NIZO food research BV, P.O.Box 20, 6710 BA Ede, The Netherlands. pMSP3535 contains the Nisin-inducible PnisA promoter, and the pAMB1 replicon for expression in gram-positive bacteria. For protein expression in E. faecalis, induce with 10-25 ng/mL nisin (Sigma, N-5764). See associated article for instructions on how to make nisin solution. Note: Nucleotide sequence encoding the signal sequence and one-third of the mature NisA (nisin) peptide remains associated with the PnisA promoter. The depositing laboratory recommends adding of a Stop codon to the 5' end of inserted sequences to avoid unintentional gene fusions. Addgene's quality control sequencing finds discrepancies with the depositor's provided sequencew which includes a mutation in RepE- F229L. This however is thought to not affect plasmid function. | Backbone Size:8353; Vector Backbone:pMSP3535; Vector Types:Bacterial Expression, Nisin-inducible expression; Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:15:57 | 6 | |||
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pQint Resource Report Resource Website |
RRID:Addgene_25819 | qQint | Erythromycin | PMID:19775243 | Between Addgene sequence and author sequence, there is a 12 nucleotide mismatched region. It is in the intron sequence, which needs to be replaced in order to use the plasmid and should not be of concern. | Backbone Size:6559; Vector Backbone:pAT19; Vector Types:bacterial gene inactivation; Bacterial Resistance:Erythromycin | plasmid to make targeted insertions in Clostridium phytofermentans chromosome using group II intron | Addgene | 2026-11-07 01:13:01 | 0 | |
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pmr101A-cPr-mNeonGreen (ErmR) Resource Report Resource Website |
RRID:Addgene_177208 | mNeonGreen | Branchiostoma lanceolatum | Erythromycin | Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR. Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning. Biotechniques. 2008 Sep;45(3):335-7. doi: 10.2144/000112907. PMID: 18778259. | Vector Backbone:pmr101A; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | Yeast codon optimized | Addgene | 2026-11-07 01:11:24 | 0 | |
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pmr101A-cPr-mCherry (ErmR) Resource Report Resource Website |
RRID:Addgene_177206 | mCherry | Discosoma | Erythromycin | Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR. Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning. Biotechniques. 2008 Sep;45(3):335-7. doi: 10.2144/000112907. PMID: 18778259. | Vector Backbone:pmr101A; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:11:24 | 0 | ||
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pCB578 Resource Report Resource Website 1+ mentions |
RRID:Addgene_141095 | Cas9 and tracrRNA | S. pyogenes | Erythromycin | PMID:30156038 | Vector Backbone:colE1, pAM beta; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:07:01 | 1 | ||
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pMSP3545-dCas9Str Resource Report Resource Website |
RRID:Addgene_153516 | dCas9Str | inactivated dCas9 from Streptococcus pneumoniae | Erythromycin | PMID:33082254 | Backbone Marker:Gary Dunny; Backbone Size:8514; Vector Backbone:pMSP3545; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:07:56 | 0 | ||
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pQlox71R Resource Report Resource Website |
RRID:Addgene_135656 | Intron containing lox site | Synthetic | Erythromycin | PMID:31826971 | Use in combination with pQlox66F (addgene 135657) or pQlox 66R (addgene 135658), then pQcre1 (addgene 135659). Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Lox66 and Lox71 must be in same orientation to delete the intervening sequence, but the directions of introns relative to one another can generate inverted repeats in the chromosome after deletion has taken place, leading to futher recombinations events. See publication for details. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439. | Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:06:08 | 0 | |
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pQcre1 Resource Report Resource Website |
RRID:Addgene_135659 | Cre recombinase | P1 phage | Erythromycin | PMID:31826971 | Cre protein under the control of the gram + PpagA promoter. Leibig M, Krismer B, Kolb M, Friede A, Götz F, Bertram R. 2008. Marker removal in staphylococci via Cre recombinase and different lox sites. Appl Environ Microbiol 74:1316– 1323. | Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:06:08 | 0 | |
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pQadd2 Resource Report Resource Website |
RRID:Addgene_135662 | Lox511/66 and LoxFas/71 | Synthetic | Erythromycin | PMID:31826971 | Cargo can be inserted between the lox sites using the SpeI and XhoI sites. | Vector Backbone:pAT19; Vector Types:Bacterial Expression, Clostridial vector, pAMβ1 origin; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:06:08 | 0 | |
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pTec_mCherry Resource Report Resource Website 1+ mentions |
RRID:Addgene_229145 | mCherry | Discosoma spp | Erythromycin | PMID:38326819 | Backbone Marker:Addgene ; Backbone Size:2800; Vector Backbone:pLp_3050sNuc; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | No | Addgene | 2026-11-07 01:33:15 | 1 | |
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pTlpA_mCherry_YafQ/DinJ Resource Report Resource Website |
RRID:Addgene_229148 | mCherry, DinJ/YafQ | Discosoma spp, Lacticaseibacillus casei | Erythromycin | PMID:36722614 | Addgene observed a ~1.4k base pair insertion downstream of the T7 terminator. This discrepancy does not affect plasmid function. | Backbone Marker:Addgene ; Vector Backbone:pLp_3050sNuc; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:33:18 | 0 | |
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PJEBAN3 Resource Report Resource Website |
RRID:Addgene_203682 | Yellow Fluorescent Protein (YFP) | Erythromycin | PMID:17014739 | Optimized version of the Cyan- and Yellow fluorescent proteins were created as follows: The hsp60 promoter driven GFP+ [19] was amplified and cloned into the pUC18 plasmid. pUC-yfp++ was obtained by introducing the following mutations by site-directed mutagenesis (Stratagene): F46L, S65G, V68A, S72A, S175G, T203Y, whereas the pUC-cfp++, was obtained by introducing the K26R, Y66W, N146I, N164H, S175G, N212K mutations | Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:28:59 | 0 | ||
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PJEBAN2 Resource Report Resource Website |
RRID:Addgene_203681 | Cyan Fluorescent Protein (CFP) | Erythromycin | PMID:17014739 | Please note that this plasmid contains an additional IS4-like element in the backbone. | Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:29:02 | 0 | ||
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pGBSedit Sham Resource Report Resource Website 1+ mentions |
RRID:Addgene_223200 | N/A | Erythromycin | PMID:40944362 | Vector Backbone:pJC005.em; Vector Types:CRISPR; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:32:59 | 2 | |||
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pGBScrispri Sham Resource Report Resource Website 1+ mentions |
RRID:Addgene_223201 | N/A | Erythromycin | PMID:40944362 | Vector Backbone:pJC005.em; Vector Types:CRISPR; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:32:59 | 2 | |||
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pEC3079 (p7INT-Pveg_lacI) Resource Report Resource Website |
RRID:Addgene_218505 | lacI | Escherichia coli | Erythromycin | PMID:38911550 | This plasmid enables the constitutive expression of LacI in S. pyogenes. It can be used for inducible gene expression in combination with the Plac promoter variants described in the associated publication. Resource information: The Pveg promoter originates from the Bacillus subtilis PY79 genome. The lacI repressor is derived from pPEPY-PF6_lacI (Plasmid #85589) and originally codon-optimised for Streptococcus pneumoniae. However, codon usage is quite similar between S. pyogenes and S. pneumoniae. p7INT was a gift from Prof. Michael Federle (University of Illinois Chicago, USA). The p7INT plasmid integrates into the 3' end of the tmRNA locus in several S. pyogenes strains. It was originally described in: McShan, W.M., McLaughlin, R.E., Nordstrand, A. et al. Vectors containing streptococcal bacteriophage integrases for site-specific gene insertion. Methods Cell Sci 20, 51–57 (1998). https://doi.org/10.1023/A:1009773309163 Please download the file linked below to obtain a well annotated map of the plasmid. | Vector Backbone:p7INT; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:31:17 | 0 | |
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pEC3135 (pERASE) Resource Report Resource Website |
RRID:Addgene_220306 | Erythromycin | PMID:38911550 | This plasmid allows to obtain scarless gene deletions in S. pyogenes and potentially other Gram-positive bacteria. The multiple cloning site (EcoRI, XbaI, SpeI, PstI) contains a Plac_mrfp cassette, allowing for red-white screening of clones in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white on LB agar plates. See the associated publication for more information. Resource Information: The pUC origin for replication in E. coli was taken from pUC19 (Plasmid #50005). The erythromycin resistance cassette was taken from p7INT. The MCS containing the mrfp cassette was taken from pSpy0C4. The coding sequence for the counterselection marker pheS* was taken from the native pheS coding sequence found in S. pyogenes SF370. This sequence was adapted to avoid recombination events of the plasmid at the phis locus due to high DNA sequence homology. Two mutations were introduced into the adapted pheS sequence to facilitate incorporation of the toxic phenylalanine analogue PCPA (4-chlor-DL-phenylalanine) into proteins, leading to bacterial cell death. p7INT was a gift from Prof. Michael Federle (University of Illinois Chicago, USA). The p7INT plasmid integrates into the 3' end of the tmRNA locus in several S. pyogenes strains. It was originally described in: McShan, W.M., McLaughlin, R.E., Nordstrand, A. et al. Vectors containing streptococcal bacteriophage integrases for site-specific gene insertion. Methods Cell Sci 20, 51–57 (1998). https://doi.org/10.1023/A:1009773309163 Please download the detailed plasmid map using the file linked below. | Backbone Size:4457; Vector Backbone:pERASE; Vector Types:Synthetic Biology; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:31:24 | 0 | |||
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pEF1-Pls3-sfGFP Resource Report Resource Website |
RRID:Addgene_205750 | Erythromycin | PMID:38335132 | Vector Backbone:pTRKH2; Vector Types:Bacterial Expression, shuttle vector gram+ gram-; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:34:50 | 0 | ||||
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pPIPL12-FnCas12a Resource Report Resource Website |
RRID:Addgene_199563 | Fncas12a | Francisella novicida | Erythromycin | PMID:37947521 | Backbone Size:4200; Vector Backbone:pGG3221; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology, Tetracycline Inducible; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:29:29 | 0 | ||
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pSR1002 Resource Report Resource Website |
RRID:Addgene_208735 | Erythromycin | PMID:38064657 | Erythromycin resistance in S. aureus | Backbone Size:4475; Vector Backbone:pSR1002; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Erythromycin | Addgene | 2026-11-07 01:29:50 | 0 |
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