Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Vector Backbone Description: Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110085 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Apramycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110088 Copy
Species: Synthetic
Genetic Insert: mCherry, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Comments: Mobilizable, stable replicating expression vector for Streptomyces, strong constitutive SF14* promoter, codon optimized Strep-tagII-mCherry gene, tsr, aac(3)IV
Proper citation: RRID:Addgene_115683 Copy
Species: Streptomyces
Genetic Insert: gusA, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Gene knock out; Bacterial Resistance:Apramycin
Comments: Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV
Proper citation: RRID:Addgene_115678 Copy
Vector Backbone Description: Backbone Size:6440; Vector Backbone:pUC19; Vector Types:Expression vector for targeted integration; Bacterial Resistance:Apramycin
Defining Citation: PMID:34890250
Comments: For cloning in homology arms, can use Gateway, restriction ligation, or homology-based cloning such as Gibson/Infusion cloning. eGFP can be swapped for your gene of interest or can be fused (recommend homology base cloning).
Original pSelAct backbone was developed here:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
pSelAct-Express was develop and tested here:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174375 Copy
Species: Synthetic
Genetic Insert: attL
Vector Backbone Description: Backbone Marker:Bierman M. et al. Gene 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.; Backbone Size:3469; Vector Backbone:pOJ260; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:35456877
Proper citation: RRID:Addgene_172193 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:6573; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:35801861
Proper citation: RRID:Addgene_190814 Copy
Species: Synthetic
Genetic Insert: dCas9-XTEN-rpoA
Vector Backbone Description: Backbone Size:6578; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:35801861
Proper citation: RRID:Addgene_190815 Copy
Species: Synthetic
Genetic Insert: AmR
Vector Backbone Description: Backbone Size:6370; Vector Backbone:pFNC-6; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39778677
Proper citation: RRID:Addgene_227670 Copy
Vector Backbone Description: Backbone Size:9558; Vector Backbone:pBAC2015, pGM1190, and pUC19; Vector Types:; Bacterial Resistance:Apramycin
Defining Citation: PMID:39666857
Proper citation: RRID:Addgene_227579 Copy
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:39666857
Proper citation: RRID:Addgene_227581 Copy
Species: Synthetic
Genetic Insert: Modified Cas9 with polyaspartate linking
Vector Backbone Description: Backbone Size:7510; Vector Backbone:pCRISPomyces-2; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39833194
Comments: Depositor notes that there are SNPs in pSG5 Rep that cause the following amino acid substitutions: L468H R467Q C354W S215A K165E H144R, but these are not of concern for plasmid function.
Proper citation: RRID:Addgene_234428 Copy
Species: Stackebrandtia nassauensis
Genetic Insert: SnaA
Vector Backbone Description: Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:38947204
Comments: encodes biosynthetic genes for threopeptin, including SnaA, SnaB, SnaC, SnaD, SnaE, SnaO, SnaT1, SnaT2
Proper citation: RRID:Addgene_225055 Copy
Genetic Insert: apramycin resistance gene
Vector Backbone Description: Vector Backbone:pSGAb; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Proper citation: RRID:Addgene_226171 Copy
Species: Stackebrandtia nassauensis
Genetic Insert: inactivated SnaE
Vector Backbone Description: Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:38947204
Comments: also encodes SnaA, SnaB, SnaC, SnaD, SnaO, SnaT1, SnaT2
Proper citation: RRID:Addgene_225057 Copy
Species: Stackebrandtia nassauensis
Genetic Insert: inactivated SnaA
Vector Backbone Description: Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:38947204
Comments: also encodes SnaB, SnaC, SnaD, SnaE, SnaO, SnaT1, SnaT2
Proper citation: RRID:Addgene_225056 Copy
Species: Synthetic
Genetic Insert: T7RNAP Circuit
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:35366417
Proper citation: RRID:Addgene_199993 Copy
Genetic Insert: msfGFP
Vector Backbone Description: Backbone Marker:SEVA collection; Backbone Size:3228; Vector Backbone:pSEVA831; Vector Types:; Bacterial Resistance:Apramycin
Defining Citation: PMID:37975669
Comments: Please visit https://doi.org/10.1101/2023.06.29.547033 for bioRxiv preprint.
Proper citation: RRID:Addgene_208016 Copy
Species: Synthetic
Genetic Insert: AcCas12n
Vector Backbone Description: Vector Backbone:p15a; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:37402371
Proper citation: RRID:Addgene_203806 Copy
Vector Backbone Description: Vector Backbone:R6Kγ; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39302127
Comments: Tn7 expression vector.
Proper citation: RRID:Addgene_222354 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within NIF that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
Welcome to the Neuroscience Information Framework Project, designed to serve the biomedical research community. NIF maintains the largest searchable collection of neuroscience data, the largest catalog of biomedical resources, and the largest ontology for neuroscience on the web. We welcome all feedback and suggestions and are actively looking for resource providers to make their resources accessible through NIF. Learn about the tools available to help you share your data and discover a dynamic inventory of Web-based neuroscience resources.
