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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
mCHOP10.pCDNA1
 
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RRID:Addgene_21899 CHOP10 Mus musculus Ampicillin and Tetracycline PMID:1547942 Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence. Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:44:28 1
CHOP 6: mCHOP-WT-9E10-pcDNA1
 
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RRID:Addgene_21913 CHOP10 Mus musculus Ampicillin and Tetracycline From depositing lab: "9E10 (Myc-epitope) tagged mouse CHOP coding region only. The insert was joined to the epitope tag using patch-PCR and incorporating unique BamH1 and Xho1 sites for ligation into pcDNA1. Stocks are provided as MC1060 (or MC1061, we are not sure) host strain transformed with the plasmid. Note that pCDNA1 plasmids must be propagated on combined amp and tet selection. I would recommedn that you consider transferring the insert into a better host strain such as pcDNA3" Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:44:28 9
pAXEF-Rap1-E63(GTP)
 
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RRID:Addgene_32698 RAP1 Homo sapiens Ampicillin and Tetracycline PMID:15469846 Q63 changed to E to give phenotype Vector Backbone:pAXEF-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline E63: Consitutively GTP-bound 2026-07-25 12:45:53 4
pRS_1N_400
 
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RRID:Addgene_170476 T7A1, O1, O-, Lambda t1 in lacZ fragment Synthetic Ampicillin and Tetracycline PMID:35188572 LacI operators separated by 400 base pairs. Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:42:37 0
pDM_N1_400
 
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RRID:Addgene_170474 T7A1, O-, O1, Lambda t1 in modified lacZ fragment Synthetic Ampicillin and Tetracycline PMID:35188572 LacI operators separated by 400 base pairs. Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline diminished hairpins near O1 and diminished latent promoters near Lambda T1 terminator 2026-07-25 12:42:37 0
pRS_SN_400
 
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RRID:Addgene_170472 T7A1, Os, O-, Lambda t1 in lacZ fragment Synthetic Ampicillin and Tetracycline LacI operators separated by 400 base pairs. Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:42:37 0
pYY_S1_400
 
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RRID:Addgene_170470 T7A1, Os, O1, lambda t1, in lacZ fragment Synthetic Ampicillin and Tetracycline PMID:28940077 LacI operators separated by 400 base pairs. Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:42:37 0
pRS_2N_400
 
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RRID:Addgene_170479 T7A1, O2, O-, Lambda t1 in lacZ fragment Synthetic Ampicillin and Tetracycline LacI operators separated by 400 base pairs. Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:42:37 0
pMOD4 RT-G
 
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RRID:Addgene_19184 rpsL tetA E.coli Ampicillin and Tetracycline PMID:19116030 Backbone Marker:Epicentre; Backbone Size:2100; Vector Backbone:pMOD4; Vector Types:Recombineering; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:44:07 0
qs5
 
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RRID:Addgene_24498 Gq alpha Mus musculus Ampicillin and Tetracycline PMID:8863834 This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gs alpha(QYELL) 2026-07-25 12:44:50 1
sq5
 
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RRID:Addgene_24499 Gs alpha Rattus norvegicus Ampicillin and Tetracycline PMID:8863834 This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase. There isn't much experience with this chimera at the moment. It may be useful for people who find the AC stimulation is better readout of receptor activation than PLC stimulation. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). 2026-07-25 12:44:50 2
qi5
 
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RRID:Addgene_24501 Gq alpha Mus musculus Ampicillin and Tetracycline PMID:8387644 This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF). This construct allows many Gi-coupled receptors to stimulate phospholipase C (PLC). This is the most popular chimera, perhaps because it is easier to talk about coupling to Gi-coupled receptors with "qi5" rather than "qo5" or "qz5." Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF) 2026-07-25 12:44:50 6
qo5
 
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RRID:Addgene_24500 Gq alpha Mus musculus Ampicillin and Tetracycline PMID:8387644 This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY). Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity. This can increase the signal-to-noise ratio. Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY) 2026-07-25 12:44:50 1
pcDNA1-Tastin
 
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RRID:Addgene_11871 Tastin Homo sapiens Ampicillin and Tetracycline PMID:7758945 Backbone Size:4033; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:36:18 0
pTU1-A-gap_RiboJ_GFP+_MGApt_Bba_B0015
 
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RRID:Addgene_107577 GFP+ Synthetic Ampicillin and Tetracycline PMID:29666238 Backbone Marker:N/A; Backbone Size:5788; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline F64L/ S65T/ Q80R/ F99S/ M153T/ V163A 2026-07-25 12:34:33 0
pCVD27
 
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RRID:Addgene_63690 ctxA Other Ampicillin and Tetracycline Kaper, J. B., J. G. Morris, Jr., and M. Nishibuchi. 1988. DNA probes for pathogenic Vibrio species, p. 65-77. In F. C. Tenover (ed.), DNA probes for infectious disease. CRC Press, Inc., Boca, Raton, Fla. Backbone Marker:NEB; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:49:53 0
pPtPBR11
 
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RRID:Addgene_80386 Ampicillin and Tetracycline Sequence is associated with the manuscript Diner et al. 2016 in review Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Synthetic Biology, : Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:52:21 1
pPtPBR2
 
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RRID:Addgene_80387 Ampicillin and Tetracycline Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Synthetic Biology, ; Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:52:21 0
Human ICAM-2
 
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RRID:Addgene_8634 ICAM-2 Homo sapiens Ampicillin and Tetracycline PMID:2497351 This clone will direct the expression of functional human ICAM-2 in a variety of cell types. This sequence has been deposited in Genbank with accession number X15606. Also see ref: Nature 329, 840-842 (1987) Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:53:11 0
Human ICAM-1
 
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RRID:Addgene_8632 ICAM-1 Homo sapiens Ampicillin and Tetracycline PMID:3349522 This clone will direct the expression of functional human ICAM-1 in a variety of cell types. This sequence has been deposited in Genbank with accession number J03132. Also see refs: Nature 329, 840-842 (1987) and Nature 339, 61-64 (1989) Please use either GenBank IDs NM_000201.1 or J03132.1 as a refence sequence for the ICAM-1 insert in this plasmid. GenBank ID NM_000201.2 (updated after this plasmid was created) is not accurate for comparison. Backbone Marker:BCCM/LMBP; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:53:11 4

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