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Species: Schizosaccharomyces pombe
Genetic Insert: urg1 promoter
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-natMX6-P3nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19355 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: urg1 promoter
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pFA6a-hphMX6-P3nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19356 Copy
Species: Caenorhabditis elegans
Genetic Insert: mes-3 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19371 Copy
Species: Caenorhabditis elegans
Genetic Insert: puf-5 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19372 Copy
Genetic Insert: Enhanced Green Fluorescent Protein
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2348; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_19364 Copy
Vector Backbone Description: Backbone Marker:Vernet et al 1987; Backbone Size:6400; Vector Backbone:pVT102U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19880801
Proper citation: RRID:Addgene_19363 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 1 alpha
Vector Backbone Description: Backbone Size:5188; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17220275
Proper citation: RRID:Addgene_19365 Copy
Species: Caenorhabditis elegans
Genetic Insert: daz-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19368 Copy
Species: Caenorhabditis elegans
Genetic Insert: tbb-2 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19401 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-142 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19367 Copy
Vector Backbone Description: Backbone Size:4764; Vector Backbone:pRK7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10934474
Proper citation: RRID:Addgene_19400 Copy
Species: Caenorhabditis elegans
Genetic Insert: spn-4 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19369 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19405 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-5 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19404 Copy
Genetic Insert: empty vector
Vector Backbone Description: Backbone Size:2932; Vector Backbone:pTREtight; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_19407 Copy
Species: Caenorhabditis elegans
Genetic Insert: fbf-2 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19406 Copy
Species: Kluyveromyces lactis
Genetic Insert: GDP1
Vector Backbone Description: Backbone Size:2932; Vector Backbone:pTREtight2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_19408 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: his3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P41nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19340 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi5605 targeting region
Vector Backbone Description: Backbone Size:2334; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Standard Multiple Cloning site vector to generate MosSCI inserts at ttTi5605 location. Contains C.Briggsae unc-119 rescue gene. Also contains genomic regions flanking the ttTi5605 Mos1 insertion. Use C.elegans strain EG4322 to generate insertions.
Proper citation: RRID:Addgene_19330 Copy
Species: Caenorhabditis elegans
Genetic Insert: Phsp-16::Mos1 transposase
Vector Backbone Description: Backbone Size:2530; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: This plasmid was made by Dr. Jean Louis Bessereau and described in the publication: Bessereau JL, Wright A, Williams DC, Schuske K, Davis MW, Jorgensen EM.
Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line.
Nature. 2001 Sep 6;413(6851):70-4.
There is che-1 sequence in the plasmid. It comes from the glh-2 3' UTR fragment in the plasmid. The UTR fragment extends into the 3' region of the che-1 gene, which is tail to tail with glh-2 on the chromosome. This is only the last exon of che-1 and it is not relevant to the function of the construct.
Proper citation: RRID:Addgene_19333 Copy
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