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Species: Homo sapiens
Genetic Insert: MS4A1
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683180
Comments: Can be used to knockout the human CD20 gene, MS4A1. After which, pCDH-V1-rCD20-BSD, pCDH-V2-rCD20-BSD or pCDH-V3-rCD20-BSD can be used to reconstitute CD20 expression with either variants 1, 2 or 3 mRNA isoforms of CD20.
Proper citation: RRID:Addgene_209749 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683180
Proper citation: RRID:Addgene_209750 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210178 Copy
Species: Homo sapiens
Genetic Insert: MS4A1
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:7133; Vector Backbone:pCDH-CMV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683180
Proper citation: RRID:Addgene_209759 Copy
Species: Homo sapiens
Genetic Insert: MS4A1
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:7133; Vector Backbone:pCDH-CMV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683180
Proper citation: RRID:Addgene_209756 Copy
Species: Homo sapiens
Genetic Insert: MS4A1
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:7133; Vector Backbone:pCDH-CMV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683180
Proper citation: RRID:Addgene_209757 Copy
Species: Homo sapiens
Genetic Insert: RILPL1
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38091401
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.07.544051v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_201470 Copy
Species: Homo sapiens
Genetic Insert: MS4A1
Vector Backbone Description: Backbone Size:7456; Vector Backbone:pCDH-IRES-BSD; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683180
Comments: Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 1 mRNA isoform of CD20. The "CCTGGGGGGTCTTCTGATGATCC" sequence within the CDS of MS4A1 was altered with synonymous substitutions to "GTTGGGCGGACTACTTATGATTC" to avoid recognition by the gRNA from LentiCRISPRv2-sgCD20. This allows for the rescue of CD20 expression after LentiCRISPRv2-sgCD20 vector-mediated knockout.
Proper citation: RRID:Addgene_209752 Copy
Species: Homo sapiens
Genetic Insert: MS4A1
Vector Backbone Description: Backbone Size:7456; Vector Backbone:pCDH-IRES-BSD; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683180
Comments: Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 2 mRNA isoform of CD20. The "CCTGGGGGGTCTTCTGATGATCC" sequence within the CDS of MS4A1 was altered with synonymous substitutions to "GTTGGGCGGACTACTTATGATTC" to avoid recognition by the gRNA from LentiCRISPRv2-sgCD20. This allows for the rescue of CD20 expression after LentiCRISPRv2-sgCD20 vector-mediated knockout.
Proper citation: RRID:Addgene_209753 Copy
Species: Homo sapiens
Genetic Insert: MS4A1
Vector Backbone Description: Backbone Size:7456; Vector Backbone:pCDH-IRES-BSD; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683180
Comments: Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform of CD20. The "CCTGGGGGGTCTTCTGATGATCC" sequence within the CDS of MS4A1 was altered with synonymous substitutions to "GTTGGGCGGACTACTTATGATTC" to avoid recognition by the gRNA from LentiCRISPRv2-sgCD20. This allows for the rescue of CD20 expression after LentiCRISPRv2-sgCD20 vector-mediated knockout.
Proper citation: RRID:Addgene_209754 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-W353G-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210181 Copy
Species: Selenomonas sp. isolate RGIG9219 Water_deer_Rumen (MAG), JAGBLH010000220
Genetic Insert: Cas8-HNH proteins and CRISPR array
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205966 Copy
Species: Homo sapiens
Genetic Insert: RPS2
Vector Backbone Description: Backbone Size:10863; Vector Backbone:pLIX403_Capture1_APOBEC_HA_P2A_mRuby; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33963355
Proper citation: RRID:Addgene_194703 Copy
Species: Synthetic
Genetic Insert: EGFP/BC12
Vector Backbone Description: Vector Backbone:pCER206; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37321219
Comments: Please note: Plasmid contains a M234K mutation in EGFP. This mutation is not expected to affect plasmid function.
Proper citation: RRID:Addgene_202853 Copy
Species: Sulfitobacter sp. JL08, NZ_CP025815
Genetic Insert: DinG HNH crRNA
Vector Backbone Description: Vector Backbone:pColADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205964 Copy
Species: Candidatus Cloacimonetes bacterium (MAG), MWEE01000013
Genetic Insert: Cas5-HNH proteins and CRISPR array
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205969 Copy
Species: Homo sapiens
Genetic Insert: PML (isoform IVa; 3MAS)
Vector Backbone Description: Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34795231
Comments: Control for PMLiva SUMO-ID experiments; 3MAS mutant lacks major SUMOylation sites and SIM domain.
Proper citation: RRID:Addgene_208039 Copy
Species: Homo sapiens
Genetic Insert: SUMO1nc
Vector Backbone Description: Backbone Marker:Open Biosystems; Vector Backbone:TRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34795231
Comments: Lentiviral packaging of TRIPZ is more efficient with TAT expression (e.g using pcDNA1-TatHIV Addgene ID: 138478) and 2nd generation gag/pol/env combination (e.g. using PSPAX2 Addgene ID: 12260 and pMD2.G Addgene ID: 12259); SUMO1nc is excisable using EcoR1-Mlu1.
Proper citation: RRID:Addgene_208035 Copy
Species: Sulfitobacter sp. JL08, NZ_CP025815
Genetic Insert: DinG HNH Cascade with His14-tagged Csf3
Vector Backbone Description: Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205963 Copy
Species: Drosophila melanogaster
Genetic Insert: HyperADARcd
Vector Backbone Description: Vector Backbone:pJG054; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34426573
Comments: Genbank: MW026677
Proper citation: RRID:Addgene_202575 Copy
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