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https://dgrc.bio.indiana.edu/product/View?product=1562
Species: Drosophila
Comments: Nanobody, pAW backbone, Nanobody expression in fly cells, GFP, CD8
Proper citation: RRID:DGRC_1562 Copy
https://dgrc.bio.indiana.edu/product/View?product=1009
Species: Drosophila
Defining Citation: PMID:11102367
Comments: Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, eGFP tag
Proper citation: RRID:DGRC_1009 Copy
https://dgrc.bio.indiana.edu/product/View?product=1012
Species: Drosophila
Comments: Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, V5 tag, 6X His tag
Proper citation: RRID:DGRC_1012 Copy
https://dgrc.bio.indiana.edu/product/View?product=1011
Species: Drosophila
Defining Citation: PMID:11532957
Comments: Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, eCFP tag
Proper citation: RRID:DGRC_1011 Copy
https://dgrc.bio.indiana.edu/product/View?product=1010
Species: Drosophila
Defining Citation: PMID:10585440
Comments: Epitope Tagged Vectors, UAS/GAL4 Vectors, Recombination and Gateway Vectors, General Fly Transformation Vectors, white[+], P-elements, UAS enhancer, hsp70 promoter, SV40 3'UTR, eYFP tag
Proper citation: RRID:DGRC_1010 Copy
https://dgrc.bio.indiana.edu/product/View?product=1604016
Species: Drosophila melanogaster
Defining Citation: PMID:16326860
Comments: BDGP iPCR Collection
Proper citation: RRID:DGRC_1604016 Copy
https://dgrc.bio.indiana.edu/product/View?product=1381486
Species: Drosophila melanogaster
Defining Citation: PMID:16326860
Comments: BDGP iPCR Collection
Proper citation: RRID:DGRC_1381486 Copy
Species: Mus musculus
Genetic Insert: anti-VGlut3 (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750780. Isotype: IgG2a. Additional species: mouse, rat.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114494 Copy
Species: Mus musculus
Genetic Insert: anti-NGL-3/LRRC4B (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750799. Isotype: IgG2a. Additional species: rat, mouse.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114493 Copy
Species: Homo sapiens
Genetic Insert: MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30956114
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human MYL2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of CAGGS-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209).
For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/.
Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint.
Proper citation: RRID:Addgene_114414 Copy
Species: Homo sapiens
Genetic Insert: TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30956114
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human TNNI1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of hPGK-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209).
For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/.
Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint.
Proper citation: RRID:Addgene_114411 Copy
Species: Synthetic
Genetic Insert: iLID domain
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30182785
Proper citation: RRID:Addgene_114418 Copy
Species: Synthetic
Genetic Insert: iLID domain
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30182785
Proper citation: RRID:Addgene_114417 Copy
Species: Synthetic
Genetic Insert: iLID domain
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30182785
Proper citation: RRID:Addgene_114415 Copy
Species: Homo sapiens
Genetic Insert: MLL-AF4 fusion gene
Vector Backbone Description: Backbone Marker:Rudolf Jaenisch, Addgene Plasmid #22075; Backbone Size:6602; Vector Backbone:AAVS1 SA-2A-puro-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29386396
Proper citation: RRID:Addgene_114380 Copy
Species: Mus musculus
Genetic Insert: anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750763. Isotype: IgG2a. Additional species: mouse, rat.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114482 Copy
Species: Mus musculus
Genetic Insert: anti-SUR2B (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750775. Isotype: IgG2a. Additional species: rat, mouse, human.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114480 Copy
Species: Mus musculus
Genetic Insert: anti-Botch (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750705. Isotype: IgG2a. Additional species: rat, mouse, human.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114487 Copy
Species: Mus musculus
Genetic Insert: anti-Flrt3 (Mus musculus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750784. Isotype: IgG2a. Additional species: mouse.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114486 Copy
Species: Homo sapiens
Genetic Insert: LMNB1 Homology Arms with mTagRFPT-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human LMNB1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRAQAS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_114403 Copy
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