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Vector Backbone Description: Vector Backbone:pSynORI; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:41629321
Comments: J23119-Repressor (F3m1)-RNA Primer (-68nt)-Target (F3m1)-J23110-parDE-parCBA-AprR
Proper citation: RRID:Addgene_253209 Copy
Genetic Insert: SceI
Vector Backbone Description: Vector Backbone:pSW-Apr; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:36216579
Proper citation: RRID:Addgene_252219 Copy
Species: Acinetobacter baumannii
Genetic Insert: mltB
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pSuiAb; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Proper citation: RRID:Addgene_253612 Copy
Genetic Insert: gRNA targetting desD in Streptomyces
Vector Backbone Description: Backbone Marker:Addgene plasmid #125689 (Weber lab); Backbone Size:12247; Vector Backbone:pCRISPR-cBEST; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41942569
Proper citation: RRID:Addgene_219650 Copy
Genetic Insert: Protospacer targeting desD in Streptomyces
Vector Backbone Description: Backbone Marker:Addgene plasmid #125689 (Weber lab); Vector Backbone:pCRISPR-cBEST; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41942569
Proper citation: RRID:Addgene_219651 Copy
Species: Synthetic
Genetic Insert: CRISPRt transposon with gRNA expression cassette (mScar-T03 guide)
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225838 Copy
Species: Synthetic
Genetic Insert: CRISPRtOE transposon with gRNA expression cassette (no guide) and synthetic promoter H
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225854 Copy
Species: Synthetic
Genetic Insert: CRISPRtOE transposon with gRNA expression cassette (no guide) and no outward facing promoter
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225853 Copy
Species: Synthetic
Genetic Insert: Tn7 transposon with mScarlet-I expression
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, Tn7 Transposition; Bacterial Resistance:Apramycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225866 Copy
Species: Oplophorus gracilirostris
Genetic Insert: NanoLuc luciferase
Vector Backbone Description: Backbone Marker:WT; Backbone Size:60095; Vector Backbone:RP4; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Proper citation: RRID:Addgene_258682 Copy
Vector Backbone Description: Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110085 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Apramycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110088 Copy
Species: Synthetic
Genetic Insert: mCherry, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Comments: Mobilizable, stable replicating expression vector for Streptomyces, strong constitutive SF14* promoter, codon optimized Strep-tagII-mCherry gene, tsr, aac(3)IV
Proper citation: RRID:Addgene_115683 Copy
Species: Streptomyces
Genetic Insert: gusA, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Gene knock out; Bacterial Resistance:Apramycin
Comments: Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV
Proper citation: RRID:Addgene_115678 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:25806970
Comments: Please note that an IS1 transposase sequence was found downstream of Cas9 during Addgene's quality control. The depositor noted that this sequence should NOT affect plasmid function.
Proper citation: RRID:Addgene_125688 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spdCas9, sgRNA casette
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:25806970
Proper citation: RRID:Addgene_125687 Copy
Vector Backbone Description: Backbone Size:5613; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126044 Copy
Vector Backbone Description: Backbone Size:5681; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126595 Copy
Vector Backbone Description: Backbone Size:5220; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126596 Copy
Species: Synthetic
Genetic Insert: tcp830
Vector Backbone Description: Backbone Size:4460; Vector Backbone:pSET152; Vector Types:Streptomyce integration vector derived from phiC31 int/attP; Bacterial Resistance:Apramycin
Defining Citation: PMID:15917435
Comments: For research using this plasmid, please acknowledge the depositing laboratory and associated citation.
Proper citation: RRID:Addgene_127088 Copy
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