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Species: Mus musculus
Genetic Insert: Nfkb2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20019 Copy
Species: Mus musculus
Genetic Insert: RelB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20017 Copy
Species: Mus musculus
Genetic Insert: RelA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See cFlag pcDNA plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20012 Copy
Species: Mus musculus
Genetic Insert: RelA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20015 Copy
Species: Mus musculus
Genetic Insert: Notch 3 Intracellular Domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD3 was cloned into XbaI/BamHI sites. NICD3 (Met-1664 to Ala-2318 of full-length mouse N3) was amplified from the N3 plasmid (gift from Dr. U. Lendahl) with 5XbaN3 (5'-GCTCTAGAATGGTTGCCAGGCGAAAGCGAG-3') and 3BamN3
(5'-GCGGATCCCTAGGCCATCACCTGCCTCTTGGG-3') primers.
Proper citation: RRID:Addgene_20185 Copy
Species: Mus musculus
Genetic Insert: Notch 2 intracellular domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD2 was cloned into XbaI/BamHI sites. NICD2 (Met-1699 to Ala-2470 of full-length mouse N2) was amplified from the N2 plasmid (gift from Dr. Y. Hamada) using primers 5XbaN2 (5'-GCTCTAGAATGGCCAAGCGGAAGCGCAAG-3') and 3BamN2
(5'-GCGGATCCCTATGCATACACCTGCATGTTGCTG-3').
Please note that Addgene sequencing results confirm the updated domain numbering to be Met 1701-Ala 2473.
Proper citation: RRID:Addgene_20184 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS1B
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20239 Copy
Species: Mus musculus
Genetic Insert: SV40LgT
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:4663; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20204 Copy
Species: Mus musculus
Genetic Insert: C-Fluc
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:6082; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20203 Copy
Species: Mus musculus
Genetic Insert: pDEST27-Vinculin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20144 Copy
Species: Mus musculus
Genetic Insert: pcDNA3.1/nV5-DEST-beta catenin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7136; Vector Backbone:pcDNA3.1/nV5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20140 Copy
Species: Mus musculus
Genetic Insert: CFP-FKBP-p85
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:ECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20159 Copy
Species: Mus musculus
Genetic Insert: mIL-17
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4242; Vector Backbone:pGL4.10; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20127 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS4
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20243 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS2
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20241 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS1C
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20240 Copy
Species: Mus musculus
Genetic Insert: Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:7222; Vector Backbone:FUW (Lenti-Lox-Ubi); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20328 Copy
Species: Mus musculus
Genetic Insert: Sox2-F2A-Oct4-T2A-Klf4
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:7222; Vector Backbone:FUW (Lenti-Lox-Ubi); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20327 Copy
Species: Mus musculus
Genetic Insert: Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:7222; Vector Backbone:FUW (Lenti-Lox-Ubi); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20325 Copy
Species: Mus musculus
Genetic Insert: Sox2-F2A-Oct4
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:7222; Vector Backbone:FUW (Lenti-Lox-Ubi); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20329 Copy
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