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Species: E. coli
Genetic Insert: sgGal4
Vector Backbone Description: Backbone Marker:Addgene #51024; Vector Backbone:pSLQ1651-sgRNA(F+E); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28841410
Proper citation: RRID:Addgene_100549 Copy
Species: E. coli
Genetic Insert: RelA fragment from E.coli
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29241543
Proper citation: RRID:Addgene_100916 Copy
Species: E. coli
Genetic Insert: Ptrc promoter driving GalP transporter expression
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pAM1303; Vector Types:Cloning vector; Bacterial Resistance:Nourseothricin (clonNat)
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101843 Copy
Species: E. coli
Genetic Insert: Formaldehyde repressor protein (frmR)
Vector Backbone Description: Vector Backbone:pTR47m4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28921510
Proper citation: RRID:Addgene_102436 Copy
Species: E. coli
Genetic Insert: Colicin E1 and E1 immunity protein
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19029376
Comments: Insert was cloned from ~base pair 5015 to ~500 of the circular ColE1 plasmid, where numbering begins within the colicin E1 gene. The ColE1 sequences include both the promoter for colicin E1 and for E1 immunity protein, which is transcribed in the opposite direction from the colicin gene. The cloned DNA does not include the intact kil (lysis protein) gene.
Proper citation: RRID:Addgene_103063 Copy
Species: E. coli
Genetic Insert: colicin E1 immunity protein
Vector Backbone Description: Backbone Marker:Novagen EMD/Millipore; Backbone Size:5627; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27795317
Comments: The genes for colicin E1 with the R domain deletion and E1 immunity protein are transcribed from opposite strands of the vector. Colicin synthesis is under control of the vector inducible promoter; immunity protein synthesis is controlled by its own promoter.
Proper citation: RRID:Addgene_103065 Copy
Species: E. coli
Genetic Insert: rpoA-rpoB-rpoC-6xHis and rpoZ
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25665556
Comments: Plasmid pVS10 encodes the E. coli rpoA-rpoB-rpoC [His6] and
rpoZ ORFs under control of T7 promoter.
Proper citation: RRID:Addgene_104398 Copy
Species: E. coli
Genetic Insert: rpoA, B, C, and Z
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression, E. coli expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25665556
Comments: pIA900 (rpoA, B, C, and Z) encodes a C-terminally His-tagged β′ which can be removed using TEV protease. Linker with TEV-His10 cloned between NheI and StuI sites of pIA787; NheI site is gone
Proper citation: RRID:Addgene_104401 Copy
Species: E. coli
Genetic Insert: RecE residues 564-866 (C-terminal nuclease domain)
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19446525
Proper citation: RRID:Addgene_104534 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105839 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105844 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105845 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105843 Copy
Species: E. coli
Genetic Insert: Maltose-binding protein (MalE)
Vector Backbone Description: Backbone Marker:IBA GmbH; Backbone Size:3286; Vector Backbone:pASK-IBA2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30600549
Comments: Spycatcher was published in Zakeri et al. (2012), PNAS 109, ppE690-7
Proper citation: RRID:Addgene_107422 Copy
Species: E. coli
Genetic Insert: yfaL
Vector Backbone Description: Backbone Size:3986; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22344647
Comments: Addgene QC finds S92G, G99S, and K330R mutations in yfaL as well as a stop codon inserted before the His tag. The depositor notes these are not of functional concern
Proper citation: RRID:Addgene_110287 Copy
Species: E. coli
Genetic Insert: OmpW
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18641391
Proper citation: RRID:Addgene_112121 Copy
Species: E. coli
Genetic Insert: OmpT
Vector Backbone Description: Backbone Size:5636; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18641391
Proper citation: RRID:Addgene_112123 Copy
Species: E. coli
Genetic Insert: 2(KW)2-mCherry
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26264666
Comments: Note: The mCherry insert contains a N9Q mutation. The depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_112738 Copy
Species: E. coli
Genetic Insert: 2HMG-eGFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_112737 Copy
Species: E. coli
Genetic Insert: neoPheS
Vector Backbone Description: Backbone Size:3470; Vector Backbone:pPB_LR5; Vector Types:Mammalian Expression, piggyBac transposon Gateway destination vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30477585
Proper citation: RRID:Addgene_112923 Copy
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