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Species: Other
Genetic Insert: pCue-sfGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167223 Copy
Species: Other
Genetic Insert: pPbr-sfGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167222 Copy
Species: Other
Genetic Insert: J23119-AKSIRV
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_167227 Copy
Species: Other
Genetic Insert: pIRH-sfGFP
Vector Backbone Description: Vector Backbone:pJL1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_167226 Copy
Species: Other
Genetic Insert: ArsR EP3
Vector Backbone Description: Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://pubs.acs.org/doi/full/10.1021/acs.est.5b00832?src=recsys
Proper citation: RRID:Addgene_167214 Copy
Species: Other
Genetic Insert: MerR
Vector Backbone Description: Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_167213 Copy
Species: Other
Genetic Insert: pArs-sfGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167219 Copy
Species: Other
Genetic Insert: pT7-XylE
Vector Backbone Description: Vector Backbone:pJL1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_167241 Copy
Species: Other
Genetic Insert: pMer-AKSIRV
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167235 Copy
Species: Other
Genetic Insert: pT7-sfGFP-MGA
Vector Backbone Description: Vector Backbone:pJL1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_167239 Copy
Species: Other
Genetic Insert: J23119-sfGFP-MGA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167238 Copy
Species: Other
Genetic Insert: pMer-XylE
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167252 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40121079
Comments: mCherry
Proper citation: RRID:Addgene_226861 Copy
Species: Other
Genetic Insert: ABE8.20-NG
Vector Backbone Description: Vector Backbone:piggyBac; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40121079
Proper citation: RRID:Addgene_226866 Copy
Species: Other
Genetic Insert: ancBE4max-NG
Vector Backbone Description: Vector Backbone:piggyBac; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40121079
Proper citation: RRID:Addgene_226865 Copy
Species: Other
Genetic Insert: evoBE4max-NG
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40121079
Proper citation: RRID:Addgene_226852 Copy
Species: Other
Genetic Insert: ABE8e-SpRY
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40121079
Proper citation: RRID:Addgene_226858 Copy
Species: Other
Genetic Insert: ABE8e-NG
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40121079
Proper citation: RRID:Addgene_226856 Copy
Species: Other
Genetic Insert: ancBE4max-SpRY
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40121079
Proper citation: RRID:Addgene_226855 Copy
Species: Other
Genetic Insert: ER-TurboID-V5
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214453 Copy
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