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http://www.addgene.org/41392

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:10065; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Plasmid for constitutive lentiviral expression. There is a C-terminal V5 tag followed by a stop codon directly after the Gateway sequence. Alternate plasmid names: pLX307, SV40-puro; EF1a-gateway-V5 tag

Proper citation: RRID:Addgene_41392 Copy   

•   Source: Addgene

http://www.addgene.org/41391

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9308; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Plasmid for constitutive lentiviral expression. There is a C-terminal V5 tag followed by a stop codon directly after the Gateway sequence. Alternate plasmid names: pLX306, SV40-puro; PGK-gateway-V5 tag

Proper citation: RRID:Addgene_41391 Copy   

•   Source: Addgene

http://www.addgene.org/41395

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Addgene plasmid 41393; Backbone Size:9396; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination vector, Doxycycline inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: The plasmid expresses puro-2A-rtTA-VP16 from the PGK promoter, so it can be used as an 'all-in-one' dox on system. Alternate plasid name: TRE-gateway-V5; PGK-puro-2A-rtTA

Proper citation: RRID:Addgene_41395 Copy   

•   Source: Addgene

http://www.addgene.org/41894

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid# 14140); Backbone Size:6972; Vector Backbone:pAG306GPD-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This plasmid was generated by ligation of a SmaI-digested fusion PCR product that contained two ~500-base-pair regions of chromosome 1 flanking a NotI site into AatII-digested pAG306-GPD-ccdB.

Proper citation: RRID:Addgene_41894 Copy   

•   Source: Addgene

http://www.addgene.org/42161

Species:
Genetic Insert: TAP Tag::mTFP1
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.

Proper citation: RRID:Addgene_42161 Copy   

•   Source: Addgene

http://www.addgene.org/42152

Species:
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.

Proper citation: RRID:Addgene_42152 Copy   

•   Source: Addgene

http://www.addgene.org/30325

Species: phage phiC31
Genetic Insert: phiC31 attB site
Vector Backbone Description: Backbone Size:4670; Vector Backbone:pDEST; Vector Types:Drosophila transgenesis; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Gateway destination vector with phiC31 attB site, for Drosophila transformation into attP landing sites.

Proper citation: RRID:Addgene_30325 Copy   

•   Source: Addgene

http://www.addgene.org/22423

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5808; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pCS2+ was digested with Stu I and ligated with the Reading Frame B Gateway cassette

Proper citation: RRID:Addgene_22423 Copy   

•   Source: Addgene

http://www.addgene.org/22424

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pCS2+; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pCSDest was digested with PstI and XhoI to remove the attR2 site and was replaced with an attR3 site from pDESTR4-R3

Proper citation: RRID:Addgene_22424 Copy   

•   Source: Addgene

http://www.addgene.org/33298

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3744; Vector Backbone:pBPXcm-1; Vector Types:Plant expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_33298 Copy   

•   Source: Addgene

http://www.addgene.org/34914

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms. This vector does not contain a visual marker on the backbone and can be used when the gene of interest has visual phenotype. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34914 Copy   

•   Source: Addgene

http://www.addgene.org/34918

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: New version of Puromycin resistance vector for drug selection in worms. This vector replaces pBCN22-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells, and the visual marker that expresses well in non-elegans Caenorhabditis species). This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34918 Copy   

•   Source: Addgene

http://www.addgene.org/34917

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: New version of Puromycin resistance vector for drug selection in worms. This vector replaces pBCN21-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells). This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34917 Copy   

•   Source: Addgene

http://www.addgene.org/34915

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms. This vector contains a Pmyo-2::mCherry::myo-2_3'UTR pharyngeal marker on the backbone. Worm strains generated with vector can be crossed with strains generated with other visual markers such as in pBCN41-R4R3 which has a green fluorescent pharyngeal marker. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34915 Copy   

•   Source: Addgene

http://www.addgene.org/34865

Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.

Proper citation: RRID:Addgene_34865 Copy   

•   Source: Addgene

http://www.addgene.org/34863

Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.

Proper citation: RRID:Addgene_34863 Copy   

•   Source: Addgene

http://www.addgene.org/34920

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: New version of Neomycin resistance vector for drug selection in worms. This vector replaces pBCN24-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells, and the visual marker that expresses well in non-elegans Caenorhabditis species). This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34920 Copy   

•   Source: Addgene

http://www.addgene.org/35203

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11976; Vector Backbone:pBID-UASC-GRM; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Cterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.

Proper citation: RRID:Addgene_35203 Copy   

•   Source: Addgene

http://www.addgene.org/35202

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11145; Vector Backbone:pBID-UASC-G; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Generation of ϕC31transgenic Drosophila for GAL4 driven expression. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter(DSCP). Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

Proper citation: RRID:Addgene_35202 Copy   

•   Source: Addgene

http://www.addgene.org/35201

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11240; Vector Backbone:pBID-UASC-FG; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of Flag epitope fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). 3 copies of the Flag epitope is fused to the N-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

Proper citation: RRID:Addgene_35201 Copy   

•   Source: Addgene