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Species: Saccharomyces cerevisiae
Genetic Insert: Cdc14/mCherry-AID(71-114)-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc14 in yeast.
A characterization guide can be found here:
https://www.nature.com/articles/s41598-017-04791-6
Detailed description of plasmid construction can be found in the Supplemental Experimental Procedures of the original paper:
http://www.cell.com/molecular-cell/pdfExtended/S1097-2765(16)30726-2
Proper citation: RRID:Addgene_102884 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: APEX2-NES
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:7500; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102951 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: bPAC
Vector Backbone Description: Vector Backbone:pNH605; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103017 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: crPDR12-3.S
Vector Backbone Description: Backbone Marker:Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR; Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103023 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S
Vector Backbone Description: Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103024 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: crHIS4-4.S
Vector Backbone Description: Backbone Marker:Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR; Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103021 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: crADE2-3.crHIS4-4.S
Vector Backbone Description: Backbone Marker:Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR; Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103020 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Csy4
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5522; Vector Backbone:p413 TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103139 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:pDTU-113; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103140 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNAs
Vector Backbone Description: Vector Backbone:dCas9v2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103141 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pAdh1-Msn2-mCherry
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pNH604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103844 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGal1-Ras2(KAN)
Vector Backbone Description: Vector Backbone:pNH604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103846 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100989 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100986 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100991 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Size:5868; Vector Backbone:pUDP003; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101166 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101167 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101168 Copy
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