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Species: Escherichia coli
Genetic Insert: ClpX
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Depositor confirms K408E compared to NC_000913.3 has no impact on function.
Proper citation: RRID:Addgene_111514 Copy
Species: Escherichia coli
Genetic Insert: FtnA
Vector Backbone Description: Vector Backbone:plasmid PAtagRFP-actin (Verkhusha lab/Addgene); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_108969 Copy
Species: Escherichia coli
Genetic Insert: Acetyl-CoA synthetase (ACS)
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Wild Type Gene
Proper citation: RRID:Addgene_108943 Copy
Species: Escherichia coli
Genetic Insert: fliC promoter fused with GFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_115674 Copy
Species: Escherichia coli
Genetic Insert: AraC
Vector Backbone Description: Backbone Size:2147; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please note that pSB1A2 was used to create the plasmid rather than pSB1A3. This does not affect function as described in the associated publication.
Proper citation: RRID:Addgene_116850 Copy
Species: Escherichia coli
Genetic Insert: rmtB
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: JoVE antibiotic dereplication paper: Zubyk, H.L., Cox, G., Wright, G.D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e60536, doi:10.3791/60536 (2019). (https://www.jove.com/video/60536/antibiotic-dereplication-using-the-antibiotic-resistance-platform)
Proper citation: RRID:Addgene_112880 Copy
Species: Escherichia coli
Genetic Insert: NusG
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_113122 Copy
Species: Escherichia coli
Genetic Insert: Rho
Vector Backbone Description: Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_113121 Copy
Species: Escherichia coli
Genetic Insert: NusG
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_113124 Copy
Species: Escherichia coli
Genetic Insert: bacterial mechanosensitive ion channel of large conductance
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, Adeno-associated viral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_107455 Copy
Species: Escherichia Coli
Genetic Insert: bacterial mechanosensitive ion channel of large conductance
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, Adeno-associated viral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_107454 Copy
Species: Escherichia coli
Genetic Insert: ftsB
Vector Backbone Description: Vector Backbone:pJKR-L-tetR; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/04/314922 for bioRxiv preprint.
Proper citation: RRID:Addgene_107559 Copy
Species: Escherichia coli
Genetic Insert: ftsA
Vector Backbone Description: Vector Backbone:pJKR-L-tetR; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please note that an IS4 transposase sequence was found between TetR and AmpR during Addgene's quality control. The depositor noted that this sequence should NOT affect plasmid function.
Please visit https://www.biorxiv.org/content/early/2018/05/04/314922 for bioRxiv preprint.
Proper citation: RRID:Addgene_107558 Copy
Species: Escherichia coli
Genetic Insert: gyrB
Vector Backbone Description: Backbone Size:15500; Vector Backbone:pKC16; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: A fragment containing gyrB, recF, dnaN, dnaA, and lambda N genes was trimmed with nuclease Bal31 and then cut with BamHI and inserted into the BamHI site of pKC16. See article for cloning details.
Proper citation: RRID:Addgene_13324 Copy
Species: Escherichia coli
Genetic Insert: RNase HI-D10R-E48R
Vector Backbone Description: Backbone Size:5044; Vector Backbone:pICE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60367 Copy
Species: Escherichia coli
Genetic Insert: RNase HI
Vector Backbone Description: Backbone Size:5044; Vector Backbone:pICE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60365 Copy
Species: Escherichia coli
Genetic Insert: YajL
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60716 Copy
Species: Escherichia coli
Genetic Insert: gusA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Matsumura lab strain number 337
Proper citation: RRID:Addgene_61156 Copy
Species: Escherichia coli
Genetic Insert: KanR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_55749 Copy
Species: Escherichia coli
Genetic Insert: KanR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:3941; Vector Backbone:pACYC177; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_55751 Copy
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