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Species: Drosophila melanogaster
Genetic Insert: kinesin heavy chain
Vector Backbone Description: Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41758 Copy
Species: Drosophila melanogaster
Genetic Insert: kinesin heavy chain
Vector Backbone Description: Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41755 Copy
Species: Drosophila melanogaster
Genetic Insert: kinesin heavy chain
Vector Backbone Description: Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41753 Copy
Species: Drosophila melanogaster
Genetic Insert: rhophilin
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41974 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila ERK ortholog
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full–length cDNA clone for ERK (rl) was obtained from the Berkeley Drosophila Genome Project (BDGP) clone collection RE08694
Proper citation: RRID:Addgene_42076 Copy
Species: Drosophila melanogaster
Genetic Insert: slpr genomic fragment
Vector Backbone Description: Backbone Size:7862; Vector Backbone:pCaSpeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: The slpr genomic rescue construct was generated by isolating a HindIII fragment of ∼14 kb from the P1 clone DS08402, including the MLK gene, and a neighboring annotated geneCG15339. This fragment was cloned transiently into pBluescript (Stratagene) and then moved into pCaSpeR4 vector for subsequent purification and germ-line transformation.
Proper citation: RRID:Addgene_42074 Copy
Species: Drosophila melanogaster
Genetic Insert: scribble
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42064 Copy
Species: Drosophila melanogaster
Genetic Insert: hopscotch
Vector Backbone Description: Backbone Marker:Gerald Rubin; Vector Backbone:pGMR; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42062 Copy
Species: Drosophila melanogaster
Genetic Insert: UAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
Vector Backbone Description: Backbone Marker:Pfeiffer et al; Backbone Size:7831; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains mTFP1.0, and not EBFP2, as suggested by the linear plasmid map image file.
Please note that Addgene's sequencing results identified a few discrepancies when compared to GenBank ID JF267350. The depositing laboratory states that these differences are not a concern for the function of the plasmid. The full plasmid sequence has been assembled and not entirely confirmed by sequencing. Additional discrepancies may exist.
This plasmid was constructed using the following restriction enyzmes:
Bgl II / Not I - loxP sites
Not I / Xho I - EGFP-HSV
Xho I / Kpn I - mTFP1.0-HA
Kpn I / Xba I - mKO2-V5
NotI is not a unique cutter--it is present twice in the plasmid. The XbaI site has been confirmed by Addgene's sequencing results, but it not present in the full plasmid sequence.
Proper citation: RRID:Addgene_29327 Copy
Species: Drosophila melanogaster
Genetic Insert: phiC31 attB, hsp70::w
Vector Backbone Description: Backbone Size:2881; Vector Backbone:pUC; Vector Types:Drosophila transgenesis; Bacterial Resistance:Ampicillin
References:
Comments: w[+] is hsp70 basal promoter; gives full w+ (red eyes), without heat shock.
Proper citation: RRID:Addgene_30326 Copy
Species: Drosophila melanogaster
Genetic Insert: SCP1 (super core promoter 1)-pUC119
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC119; Vector Types:pUC derivative - a cloning vector; Bacterial Resistance:Ampicillin
References:
Comments: There are several mismatches between depositor's sequence and Addgene sequence. The mismatches are mostly in vector and should not affect the insert.
Proper citation: RRID:Addgene_21846 Copy
Species: Drosophila melanogaster
Genetic Insert: SCP1 (super core promoter 1)-CMVenh luciferase
Vector Backbone Description: Backbone Size:5500; Vector Backbone:Vector Backbone: a MODIFIED version (constructed by us) of Invit; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22216 Copy
Species: Drosophila melanogaster
Genetic Insert: decapping protein 2, variant A
Vector Backbone Description: Backbone Marker:Elisa Izaurralde; Backbone Size:5487; Vector Backbone:pAc5.1B-lambdaN-HA; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22409 Copy
Species: Drosophila melanogaster
Genetic Insert: gawky, variant A
Vector Backbone Description: Backbone Marker:Elisa Izaurralde; Backbone Size:5459; Vector Backbone:pAc5.1B-lambdaN; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22406 Copy
Species: Drosophila melanogaster
Genetic Insert: Argonaut-1, variant A
Vector Backbone Description: Backbone Marker:Elisa Izaurralde; Backbone Size:5455; Vector Backbone:pac5.1B-lambdaN; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22420 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 14
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22763 Copy
Species: Drosophila melanogaster
Genetic Insert: micro-RNA 315
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22765 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 12
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22760 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 13b-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22762 Copy
Species: Drosophila melanogaster
Genetic Insert: micro-RNA-92b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22709 Copy
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