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Species: Danio rerio
Genetic Insert: ZebrafishCommunity-mcu-Left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TGCGAAAGTGTGTAGATC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_41286 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-per2-Left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32285; Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TCAGCACTACTGGTGTCA
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_41312 Copy
Species: E. coli
Genetic Insert: LexAp65
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2551; Vector Backbone:pDONR P5-P2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_41437 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:10065; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Plasmid for constitutive lentiviral expression.
There is a C-terminal V5 tag followed by a stop codon directly after the Gateway sequence.
Alternate plasmid names:
pLX307,
SV40-puro; EF1a-gateway-V5 tag
Proper citation: RRID:Addgene_41392 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9308; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Plasmid for constitutive lentiviral expression.
There is a C-terminal V5 tag followed by a stop codon directly after the Gateway sequence.
Alternate plasmid names:
pLX306,
SV40-puro; PGK-gateway-V5 tag
Proper citation: RRID:Addgene_41391 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Addgene plasmid 41393; Backbone Size:9396; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination vector, Doxycycline inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: The plasmid expresses puro-2A-rtTA-VP16 from the PGK promoter, so it can be used as an 'all-in-one' dox on system.
Alternate plasid name:
TRE-gateway-V5; PGK-puro-2A-rtTA
Proper citation: RRID:Addgene_41395 Copy
Species: Homo sapiens
Genetic Insert: RIP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_41389 Copy
Species: Mus musculus
Genetic Insert: RIP3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_41384 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41606 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41605 Copy
Species: S. kluyveri
Genetic Insert: His3
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41602 Copy
Species: Homo sapiens
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: 5' cloning site: HindIII
3' cloning site: NotI
Proper citation: RRID:Addgene_41567 Copy
Species: S. kluyveri
Genetic Insert: His3
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41600 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41609 Copy
Species: Homo sapiens
Genetic Insert: SIRT6
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/3PKI/
Proper citation: RRID:Addgene_41565 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ura3-GAL1-UBIYdkGFP*-SpHis5-Tim9
Vector Backbone Description: Vector Backbone:pUC118; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41560 Copy
Species: Homo sapiens
Genetic Insert: PREX2a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5523; Vector Backbone:pcDNA3.1/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41555 Copy
Species: Homo sapiens
Genetic Insert: MAL
Vector Backbone Description: Backbone Marker:Mizushima and Nagata (Osaka Bioscience Institute, Japan, 1990) (PMID: 1698283) ; Backbone Size:5685; Vector Backbone:pEF-Bos; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The depositing lab generated pEF-Bos MAL Flag from a human peripheral blood mononuclear cell (PBMC) complementary DNA library by PCR amplification and cloning. It was cloned with XhoI at the 5' end and BamHI at the 3' end. NotI can be used at the 3' end to excise the tagged CDS. The depositor's provided sequence includes some flanking EF-BOS sequence. The construct also contains a HIS tag.
Proper citation: RRID:Addgene_41554 Copy
Species: Homo sapiens
Genetic Insert: ASC
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41553 Copy
Species: Homo sapiens
Genetic Insert: TRAM
Vector Backbone Description: Backbone Marker:Mizushima and Nagata (Osaka Bioscience Institute, Japan, 1990) (PMID: 1698283) ; Backbone Size:5685; Vector Backbone:pEF-Bos; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The depositing lab generated pEF-Bos Tram Flag from a human peripheral blood mononuclear cell (PBMC) complementary DNA library by PCR amplification and cloning. It was cloned with XhoI at the 5' end and BamHI at the 3' end. NotI can be used at the 3' end to excise the tagged CDS. The construct also contains a HIS tag.
Proper citation: RRID:Addgene_41551 Copy
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