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Genetic Insert: Tetracycline Resistance Gene
Vector Backbone Description: Backbone Size:790; Vector Backbone:R6Kγ origin of replication and oriT; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37712562
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.05.543168v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_203960 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671
Proper citation: RRID:Addgene_208896 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671
Proper citation: RRID:Addgene_203678 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671
Proper citation: RRID:Addgene_203675 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671
Proper citation: RRID:Addgene_203673 Copy
Species: n/a
Genetic Insert: pyrF-/hisB-/tnaA-
Vector Backbone Description: Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39610115
Comments: Derived from US0 pyrF-/hisB- (Scot Wolfe, Addgene #12614). This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor. This strain is also has a deletion in tnaA gene and does not produce indole, verified by Kovac's Reagent.
Please visit https://doi.org/10.1101/2024.08.07.607023 for bioRxiv preprint.
Proper citation: RRID:Addgene_228512 Copy
Genetic Insert: Chloramphenicol resistance gene - 111TAG
Vector Backbone Description: Vector Backbone:pREP; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:36383641
Proper citation: RRID:Addgene_197571 Copy
Species: Synthetic
Genetic Insert: OsU6.2-sgRNA7 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199733 Copy
Species: Synthetic
Genetic Insert: TaU6-sgRNA8 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199734 Copy
Species: Synthetic
Genetic Insert: ZmU6C3-sgRNA5 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199731 Copy
Species: Synthetic
Genetic Insert: ZmU6C1-sgRNA4 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199730 Copy
Species: Synthetic
Genetic Insert: OsU3-sgRNA2 scaffold
Vector Backbone Description: Vector Backbone:pYPQ132D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199728 Copy
Species: Synthetic
Genetic Insert: TaU3-sgRNA3 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199729 Copy
Vector Backbone Description: Vector Backbone:pCBLsgRNA2; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199737 Copy
Species: Synthetic
Genetic Insert: vioABCE
Vector Backbone Description: Vector Backbone:pBB298 (parent plasmid pEB001); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_221533 Copy
Genetic Insert: sacB
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:4259; Vector Backbone:pBBR1-Tc-ELT3 (pOGG277); Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:38715147
Proper citation: RRID:Addgene_222336 Copy
Vector Backbone Description: Backbone Size:4545; Vector Backbone:BluescriptII KS+; Vector Types:Yeast Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12054889
Proper citation: RRID:Addgene_220628 Copy
Vector Backbone Description: Backbone Size:4545; Vector Backbone:BluescriptII KS+; Vector Types:Yeast Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12054889
Proper citation: RRID:Addgene_220627 Copy
Species: Synthetic
Genetic Insert: cdGreen2
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7300; Vector Backbone:pQFmcs; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:38724508
Proper citation: RRID:Addgene_221184 Copy
Genetic Insert: SUMO-sfGFP-WT
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) sfGFP-WT, uninterrupted by TAG codons, and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This control plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from a previous Ma version by containing a tetracycline efflux marker.
Proper citation: RRID:Addgene_212119 Copy
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