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Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4492; Vector Backbone:pMV261; Vector Types:Bacterial Expression, Mycobacteria expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Replicative mycobacterial destination vector for recombination cloning.
Please note that there are multiple discrepancies between Addgene's quality control sequence and the depositor's theoretical sequence. The Hmama lab assures us that these differences should not affect plasmid function.
Proper citation: RRID:Addgene_29463 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4445; Vector Backbone:pMV361; Vector Types:Bacterial Expression, Mycobacteria expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Integrative mycobacterial destination vector for recombination cloning.
Please note that there are multiple discrepancies between Addgene's quality control sequence and the depositor's theoretical sequence. The Hmama lab assures us that these differences should not affect plasmid function.
Proper citation: RRID:Addgene_29462 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7142; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Generic promoter-less destination vector
Proper citation: RRID:Addgene_29496 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Amaxa; Backbone Size:2800; Vector Backbone:pmaxGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_37631 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmcherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please note: the orientation of the MCS within this plasmid places mCherry on the c-terminus of your gene of interest.
Proper citation: RRID:Addgene_31907 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:12096; Vector Backbone:pMDC32; Vector Types:Gateway destination binary vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_32078 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:12767; Vector Backbone:pMDC32; Vector Types:Gateway destination binary vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_32077 Copy
Species: Synthetic
Genetic Insert: Rhizobium codon-optimized ABEs
Vector Backbone Description: Vector Backbone:pKm; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_170332 Copy
Species: Synthetic
Genetic Insert: Rhizobium codon-optimized CBEs
Vector Backbone Description: Vector Backbone:pKm; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_170333 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7040; Vector Backbone:pEGFPC4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: pEGFPC4 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal EGFP tag.
Proper citation: RRID:Addgene_18671 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:13430; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants
https://academic.oup.com/plphys/article/146/2/323/6107098
Please note there are two mutations in HygR- E180D and A95S. Depositor confirms these do not affect plasmid function.
Proper citation: RRID:Addgene_174050 Copy
Species:
Genetic Insert: C terminal VSV tag
Vector Backbone Description: Backbone Size:2852; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26181 Copy
Species:
Genetic Insert: C terminal HA tag
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26174 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Med21 aa1-132 for N-terminal tagging
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26183 Copy
Species: Vibrio fischeri
Genetic Insert: ECFP, EYFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6724; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors.
Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings
https://www.nature.com/articles/npre.2008.1550.2
Proper citation: RRID:Addgene_24509 Copy
Species: Aequorea victoria (Jellyfish)
Genetic Insert: Yn, Yc, ECFP, EYFP
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:7434; Vector Backbone:pVitro2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors.
Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings
Proper citation: RRID:Addgene_24510 Copy
Species: mycobacterial
Genetic Insert: Che9C RecT
Vector Backbone Description: Backbone Marker:Sabine Ehrt and Dirk Schnappinger; Backbone Size:8026; Vector Backbone:pUV15tet-O; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Induced with anhydrotetracycline, the plasmid can be used to introduce single base pair changes in the chromosomes of M. smegmatis and M. tuberculosis.
For original description of methodology, see:
van Kessel, J.C. & Hatfull, G.F. Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets.
Mol Microbiol 67, 1094-1107 (2008).
Proper citation: RRID:Addgene_107770 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMEXC3H; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_110080 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMEXC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_110081 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMINTNH3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_110074 Copy
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