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Species: Synthetic
Genetic Insert: Taz
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29650958
Proper citation: RRID:Addgene_104333 Copy
Vector Backbone Description: Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30083222
Comments: There are two additional BsaI cloning sites in the Cas9 sequences, but they should not affect the cloning process, since GoldenGate cloning can assemble four or more fragments into a backbone. The additional BsaI sites create four overhangs that are very different from the overhangs for integrating the sgRNAs, and there should be no major issues with fragment assembly or cloning efficiency.
Proper citation: RRID:Addgene_104438 Copy
Species: Other
Genetic Insert: AtDeadCAS9 D10A H840A without stop codon
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112073 Copy
Species: Other
Genetic Insert: 3xmVenus
Vector Backbone Description: Vector Backbone:pAGM1301; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112074 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pICH41258; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112080 Copy
Species: Other
Genetic Insert: TagRFP
Vector Backbone Description: Vector Backbone:pICH41258; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112081 Copy
Species: Other
Genetic Insert: AtCAS9
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis, however the amino acid sequence is as in the original AtCas9 pDe-CAS9 vector from H. Puchta lab.
Proper citation: RRID:Addgene_112070 Copy
Species: Other
Genetic Insert: eCFP
Vector Backbone Description: Vector Backbone:pAGM1301; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112083 Copy
Species: Arabidopsis thaliana
Genetic Insert: Csy4
Vector Backbone Description: Vector Backbone:pTKan-GW-R1R2; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28094975
Proper citation: RRID:Addgene_110145 Copy
Species: Arabidopsis thaliana
Genetic Insert: Csy4
Vector Backbone Description: Vector Backbone:pTKan-GW-R1R2; Vector Types:Bacterial Expression, Plant Expression, binary vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28094975
Proper citation: RRID:Addgene_110143 Copy
Species: Synthetic
Genetic Insert: Nanoluc
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110612 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110617 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110618 Copy
Species: Synthetic
Genetic Insert: GFP dropout
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110601 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110607 Copy
Species: Homo sapiens
Genetic Insert: BAF47
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30860482
Proper citation: RRID:Addgene_111181 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT2 promoter
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108444 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT1 promoter
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108441 Copy
Species: Arabidopsis thaliana
Genetic Insert: GA2ox2
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108567 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT2
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108566 Copy
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