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Species: Schmidtea mediterranea
Genetic Insert: Runt-1 (also known as runt) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning primers: F-AGTCGCAGTGGAAGAGGAAA, R-TGATGGGATTGCGAGTTGTA
Proper citation: RRID:Addgene_99089 Copy
Species: Schmidtea mediterranea
Genetic Insert: notum in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Note: The notum insert contains several mismatches compared to JF725701.1. The depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_99067 Copy
Species: Schmidtea mediterranea
Genetic Insert: ChAT (choline acetyltransferase) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning primers: F-GATCTCCTGCCACCAAAGAA, R-AACTCCATGACCCTGAATCG
Proper citation: RRID:Addgene_99069 Copy
Species: Schmidtea mediterranea
Genetic Insert: foxD in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning primers: F-CCAGGCGTTCAAAGATTCTC, R-GATTTCCCGGTTCTCTTGGT
Proper citation: RRID:Addgene_99068 Copy
Species: Schmidtea mediterranea
Genetic Insert: LDLRR-1 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning primers: F-GCCTCACAATAGCACTTTCG R-GTAAATATTGATAATATATTTAAT
Note: There is a 1.3 kb deletion in the LDLRR-1 insert compared to KY024334.1. The depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_99093 Copy
Species: Schmidtea mediterranea
Genetic Insert: F-spondin in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning primers: F-AAACAAAAGAGCATGGTTCA, R-AATCTTGCAAACATTCGAGA
Note: Only 143 base pairs from the insert align with KY024329.1. The depositor has confirmed this does not impact plasmid function.
Proper citation: RRID:Addgene_99096 Copy
Species: Schmidtea mediterranea
Genetic Insert: CNG1 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning primers: F-AATTGTCGAGCCCTTGGTAG, R-CCCAAATTTTTCGTTTAAAGGTT
Proper citation: RRID:Addgene_99098 Copy
Species: Schmidtea mediterranea
Genetic Insert: slc1a-5 (also known as excitatory amino acid transporter) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning primers: F-gtcaaatgggaaaggaagca R-attaattgtggcgcctatcg
Proper citation: RRID:Addgene_99102 Copy
Species: Schmidtea mediterranea
Genetic Insert: estrella in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning primers: F-GAAGTCAATGGAACAGACGA, R-GAATTAGTGAGCGACCAGAA
Note: The estrella insert contains several mismatches compared to KY024338.1. The depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_99104 Copy
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