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Species: HSV-1
Genetic Insert: UL8 and UL29 gRNA, EGFP
Vector Backbone Description: Vector Backbone:pCCL-PGK-egfp; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33432198
Proper citation: RRID:Addgene_166694 Copy
Species: Mus musculus
Genetic Insert: BimL
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBimL" (Venus fused to the N-terminus of the BH3-only protein, BimL) in live cells. There is a 3 amino acid flexible linker between Venus and BimL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166734 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: tRNA promoter : sgRNA cassette
Vector Backbone Description: Backbone Size:5444; Vector Backbone:pLSB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313420
Comments: The following acknowledgement must be included in all research publications and other outputs: “pLSB-HYG was created with the support of the Wellcome Trust [095021], [200885], [203149]”.
The following statement must be included on all submissions of original research to peer-reviewed journals: “pLSB-HYG was created with the support of the Wellcome Trust [095021], [200885], [203149]”.
Proper citation: RRID:Addgene_166699 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: tRNA promoter : sgRNA cassette
Vector Backbone Description: Backbone Size:4988; Vector Backbone:pLSB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313420
Comments: The following acknowledgement must be included in all research publications and other outputs: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”.
The following statement must be included on all submissions of original research to peer-reviewed journals: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”.
Proper citation: RRID:Addgene_166698 Copy
Species: Synthetic
Genetic Insert: dCas12a-NLS
Vector Backbone Description: Backbone Size:7206; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34197613
Proper citation: RRID:Addgene_166731 Copy
Species: Homo sapiens
Genetic Insert: Bik
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VBik" (Venus fused to the N-terminus of the BH3-only protein, Bik) in live cells. There is an 8 amino acid flexible linker between Venus and Bik proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166737 Copy
Species: Mus musculus
Genetic Insert: tBid
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VtBid" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. There is a 3 amino acid flexible linker between Venus and tBid proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166736 Copy
Species: Mus musculus
Genetic Insert: BimEL
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBimEL" (Venus fused to the N-terminus of the BH3-only protein, BimEL) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166735 Copy
Species: Homo sapiens
Genetic Insert: Puma
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VPuma" (Venus fused to the N-terminus of the BH3-only protein, Puma) in live cells. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. In cloning, the last amino acid of Venus protein was deleted (K239S). Result is a 1 amino acid linker between Venus and Puma proteins. The encoded BBC3 isoform 4 is also known as PUMA-alpha.
Proper citation: RRID:Addgene_166739 Copy
Species: HSV-1
Genetic Insert: UL8 and UL29 gRNA
Vector Backbone Description: Vector Backbone:pCCL-PGK-egfp; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33432198
Proper citation: RRID:Addgene_166685 Copy
Species: Mus musculus
Genetic Insert: GFP-MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166683 Copy
Species: Mus musculus
Genetic Insert: Kitl
Vector Backbone Description: Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30504842
Proper citation: RRID:Addgene_166721 Copy
Species: Mus musculus
Genetic Insert: VP2C-GFP-MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166682 Copy
Species: Mus musculus
Genetic Insert: MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166681 Copy
Species: Mus musculus
Genetic Insert: VP2C-MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166680 Copy
Species: synthetic/engineered
Genetic Insert: muGFP
Vector Backbone Description: Backbone Size:5405; Vector Backbone:pAM-DCA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29330459
Proper citation: RRID:Addgene_166727 Copy
Species: synthetic/engineered
Genetic Insert: muGFP
Vector Backbone Description: Backbone Size:4985; Vector Backbone:pcDNA3.1+(Zeo); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29330459
Proper citation: RRID:Addgene_166726 Copy
Genetic Insert: Non-targeting guide
Vector Backbone Description: Vector Backbone:Lenti-CRISPR-V2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33101674
Proper citation: RRID:Addgene_166725 Copy
Vector Backbone Description: Vector Backbone:BPK3082; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32483191
Proper citation: RRID:Addgene_166724 Copy
Species: Homo sapiens
Genetic Insert: Mcl-1
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: 2nd generation lentiviral transfer plasmid that can be used to create lentivirus, for CMV driven expression of mCerulean3 fused to the N-terminus of Mcl-1. There is a flexible, 17 amino acid linker between mCerulean3 and Mcl-1. proteins.
Proper citation: RRID:Addgene_166752 Copy
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