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Species: Homo sapiens
Genetic Insert: ZNF627
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176365 Copy
Species: Homo sapiens
Genetic Insert: SOX11
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: PGP1 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176360 Copy
Species: Homo sapiens
Genetic Insert: ZIC3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: PGP1 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176361 Copy
Species: Synthetic
Genetic Insert: super folder green fluorescent protein with FRT-flanked ampicillin cassette
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3700; Vector Backbone:pEGFP-C1; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please note depositor confirms H217R in insert does not affect plasmid function.
Proper citation: RRID:Addgene_176620 Copy
Species: Bacillus subtilis and Bacillus methanolicus (for plasmid gene insert)
Genetic Insert: BsMDH BmHPS BmPHI (on Addgene plasmid # 129104)
Vector Backbone Description: Backbone Marker:Eleftherios Terry Papoutsakis (Addgene plasmid # 129104); Vector Backbone:pETM6_Ptrc_BsMdh_BmHps_BmPhi; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: This T-B18 E.coli strain harbors the pETM6_Ptrc_BsMdh_BmHps_BmPhi plasmid (Addgene plasmid # 129104), which enables the evolved E. coli strain to metabolize methanol in its central carbon metabolism. It was adaptively evolved from the parent strain, E. coli BW25113 ΔfrmA::kanR (obtained from the Keio collection) containing Addgene plasmid # 129104 such that it grows efficiently with just 2mM Thr and 250 mM MeOH.
This strain has been functionally validated for growth by the depositing laboratory. Addgene has not confirmed the plasmid by QC sequence analysis.
Proper citation: RRID:Addgene_159446 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:PCR4-TOPO; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please note that there are minor differences between the Addgene verified result and the depositor's reference sequence (under the supplemental documents section). Please refer to the Addgene NGS result for more accurate sequence information. These differences do not affect the plasmid function.
Proper citation: RRID:Addgene_139098 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:PCR4-TOPO; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_139099 Copy
Species: Synthetic
Genetic Insert: dLbCas12a(D156R)-VPR
Vector Backbone Description: Backbone Marker:DOI: 10.1039/C8SC02870B; Backbone Size:14777; Vector Backbone:pYFAC-riboB; Vector Types:CRISPR, Synthetic Biology, Expression in Aspergillus nidulans and related filamentous fungi.; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_140198 Copy
Species: Other
Genetic Insert: OsTIR1-F74A
Vector Backbone Description: Vector Backbone:pDB4697; Vector Types:Other; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.19.452993v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171127 Copy
Species: S. pombe (fission yeast)
Genetic Insert: ura4
Vector Backbone Description: Backbone Marker:TransGen Biotech; Vector Backbone:pEASY-blunt; Vector Types:Other; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.19.452993v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171125 Copy
Species:
Genetic Insert: TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab; Vector Backbone:pGG211; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_165615 Copy
Species:
Genetic Insert: TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab; Vector Backbone:pGG211; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_165616 Copy
Species: Homo sapiens
Genetic Insert: TP63
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: A549
Proper citation: RRID:Addgene_132477 Copy
Species: Homo sapiens
Genetic Insert: ZNF449
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: K562
Proper citation: RRID:Addgene_132515 Copy
Species: Homo sapiens
Genetic Insert: ZNF302
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: SK-N-SH_RA
Proper citation: RRID:Addgene_132513 Copy
Species: Homo sapiens
Genetic Insert: CAMTA2
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: K562
Proper citation: RRID:Addgene_132501 Copy
Species: Homo sapiens
Genetic Insert: CREB5
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: SK-N-SH
Proper citation: RRID:Addgene_132521 Copy
Species:
Genetic Insert: Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda
Vector Backbone Description: Backbone Size:2957; Vector Backbone:pUC19; Vector Types:λ-phage recombineering plasmid; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please note that there are several discrepancies between Addgene's quality control and the depositor's sequence that affects restriction enzyme sites.
Proper citation: RRID:Addgene_133935 Copy
Species: bacteriophage P1, EMCV
Genetic Insert: Cre-IRES
Vector Backbone Description: Backbone Marker:Derived in Kotton lab; Backbone Size:7830; Vector Backbone:pHAGE2; Vector Types:Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_126700 Copy
Species: Aspergillus terreus, Escherichia coli, Komagataella phaffii
Genetic Insert: slovA,cpr
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9004; Vector Backbone:pPIC3.5K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_126744 Copy
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