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Plasmids are provided by Addgene and DGRC.

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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pSR322.3
 
Resource Report
Resource Website
RRID:Addgene_125118 uhpA(REC)-ydfI(DBD)129 Synthetic Chloramphenicol PMID:31110305 Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:51 0
pKD280
 
Resource Report
Resource Website
RRID:Addgene_125071 SO_4387 Spectinomycin PMID:31110305 Depositor confirms mutation does not affect function. Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-08 01:06:50 0
pKD278
 
Resource Report
Resource Website
RRID:Addgene_125070 SO_2192 Spectinomycin PMID:31110305 Depositor confirms mutation does not affect function Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin Mutation in LacI S286L- Please see depositor comments below 2026-08-08 01:06:50 0
pKmK.I2R
 
Resource Report
Resource Website
RRID:Addgene_125064 Right homology arm for targeting integration to chromosome V: 23743..21744 Other Chloramphenicol PMID:31134195 Meant for the construction of integrative vectors (single- or multi-gene). Contains the appropriate YTK-compatible Golden Gate overhangs for a type 1 part. The necessary internal BsmBI sites to use this as a connector for multi-gene assembly have been added by PCR. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKmK.C4
 
Resource Report
Resource Website
RRID:Addgene_125062 KmARS2 Other Chloramphenicol PMID:31134195 Contains the appropriate YTK-compatible Golden Gate overhangs for a type 7 part. Please Note: Some of the bases in the native sequence were modified to eliminate an endogeneous BsaI site that interfered with Golden Gate cloning, but plasmids using the modified ARS have been stable so far in experiments. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:51 0
pKD283.2
 
Resource Report
Resource Website
RRID:Addgene_125101 SO_4487(REC)-psdR(DBD)137 Synthetic Chloramphenicol PMID:31110305 Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKD276
 
Resource Report
Resource Website
RRID:Addgene_125069 SO_0621 Spectinomycin PMID:31110305 Depositor confirms mutation does not affect function Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-08 01:06:50 0
pKD285
 
Resource Report
Resource Website
RRID:Addgene_125102 SO_4623(REC)-psdR(DBD)137 Synthetic Chloramphenicol PMID:31110305 Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:51 0
pKD281
 
Resource Report
Resource Website
RRID:Addgene_125100 SO_4428(REC)-psdR(DBD)137 Synthetic Chloramphenicol PMID:31110305 Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pSR357.10
 
Resource Report
Resource Website
RRID:Addgene_125106 narL(REC)-ydfI(DBD)131 Synthetic Chloramphenicol PMID:31110305 Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pSR357.12
 
Resource Report
Resource Website
RRID:Addgene_125107 narL(REC)-ydfI(DBD)134 Synthetic Chloramphenicol PMID:31110305 Depositor confirms mutations in sfGFP and TetR do not affect function Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKmK.C3
 
Resource Report
Resource Website
RRID:Addgene_125061 KmARS/CEN6 Other Chloramphenicol PMID:31134195 Contains the appropriate YTK-compatible Golden Gate overhangs for a type 7 part. Equivalent to the ARS2/CEN sequence from strain ATCC 12424 Please note: Addgene NGS finds a mixed population (C/T) at single nucleotide in KmARS/Cen6. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKmK.T1
 
Resource Report
Resource Website
RRID:Addgene_125053 INU1 terminator Other Chloramphenicol PMID:31134195 Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKmK.T2
 
Resource Report
Resource Website
RRID:Addgene_125054 LAC4 terminator Other Chloramphenicol PMID:31134195 Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKmK.P18
 
Resource Report
Resource Website
RRID:Addgene_125051 XYL2 promoter Other Chloramphenicol PMID:31134195 Promoter inducible by xylose. Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKmK.T5
 
Resource Report
Resource Website
RRID:Addgene_125057 PGK1 terminator Other Chloramphenicol PMID:31134195 Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKmK.M1
 
Resource Report
Resource Website
RRID:Addgene_125058 ScTRP1 marker Saccharomyces cerevisiae Chloramphenicol PMID:31134195 Contains the appropriate YTK-compatible Golden Gate overhangs for a type 6 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKmK.T3
 
Resource Report
Resource Website
RRID:Addgene_125055 KMXK_A3020 terminator Other Chloramphenicol PMID:31134195 Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKmK.T4
 
Resource Report
Resource Website
RRID:Addgene_125056 PDC1 terminator Other Chloramphenicol PMID:31134195 Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0
pKmK.P17
 
Resource Report
Resource Website
RRID:Addgene_125050 XYL1 promoter Other Chloramphenicol PMID:31134195 Promoter inducible by xylose. Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function. Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-08 01:06:50 0

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