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Species: Synthetic
Genetic Insert: uhpA(REC)-ydfI(DBD)129
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125118 Copy
Genetic Insert: SO_4387
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Comments: Depositor confirms mutation does not affect function.
Proper citation: RRID:Addgene_125071 Copy
Genetic Insert: SO_2192
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Comments: Depositor confirms mutation does not affect function
Proper citation: RRID:Addgene_125070 Copy
Species: Other
Genetic Insert: Right homology arm for targeting integration to chromosome V: 23743..21744
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Meant for the construction of integrative vectors (single- or multi-gene). Contains the appropriate YTK-compatible Golden Gate overhangs for a type 1 part. The necessary internal BsmBI sites to use this as a connector for multi-gene assembly have been added by PCR. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125064 Copy
Species: Other
Genetic Insert: KmARS2
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 7 part.
Please Note: Some of the bases in the native sequence were modified to eliminate an endogeneous BsaI site that interfered with Golden Gate cloning, but plasmids using the modified ARS have been stable so far in experiments. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125062 Copy
Species: Synthetic
Genetic Insert: SO_4487(REC)-psdR(DBD)137
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125101 Copy
Genetic Insert: SO_0621
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Comments: Depositor confirms mutation does not affect function
Proper citation: RRID:Addgene_125069 Copy
Species: Synthetic
Genetic Insert: SO_4623(REC)-psdR(DBD)137
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125102 Copy
Species: Synthetic
Genetic Insert: SO_4428(REC)-psdR(DBD)137
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125100 Copy
Species: Synthetic
Genetic Insert: narL(REC)-ydfI(DBD)131
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125106 Copy
Species: Synthetic
Genetic Insert: narL(REC)-ydfI(DBD)134
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Comments: Depositor confirms mutations in sfGFP and TetR do not affect function
Proper citation: RRID:Addgene_125107 Copy
Species: Other
Genetic Insert: KmARS/CEN6
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 7 part. Equivalent to the ARS2/CEN sequence from strain ATCC 12424
Please note: Addgene NGS finds a mixed population (C/T) at single nucleotide in KmARS/Cen6. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125061 Copy
Species: Other
Genetic Insert: INU1 terminator
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125053 Copy
Species: Other
Genetic Insert: LAC4 terminator
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125054 Copy
Species: Other
Genetic Insert: XYL2 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Promoter inducible by xylose. Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125051 Copy
Species: Other
Genetic Insert: PGK1 terminator
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125057 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScTRP1 marker
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 6 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125058 Copy
Species: Other
Genetic Insert: KMXK_A3020 terminator
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125055 Copy
Species: Other
Genetic Insert: PDC1 terminator
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125056 Copy
Species: Other
Genetic Insert: XYL1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Promoter inducible by xylose. Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125050 Copy
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