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Species: Saccharomyces cerevisiae
Genetic Insert: SLA1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrII: 213734-213753 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166075 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of RRT8
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXV:241445..241464 (-strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166080 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Msn2-sgRNA#27
Vector Backbone Description: Vector Backbone:pYTK101; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166103 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MAT Z1
Vector Backbone Description: Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t, Daran Lab; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166100 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Msn2-sgRNA#27
Vector Backbone Description: Vector Backbone:pYTK103; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166105 Copy
Vector Backbone Description: Backbone Size:4699; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD132.59, Ligation number L4632.
Proper citation: RRID:Addgene_1661 Copy
Species: P.pyralis
Genetic Insert: hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
Vector Backbone Description: Backbone Marker:Addgene #122182; Vector Backbone:pLenti spCas9 T2A iRFP670 P2A puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35388524
Proper citation: RRID:Addgene_166134 Copy
Species: Homo sapiens
Genetic Insert: DNMT1 knock-out construct
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3264; Vector Backbone:pSL301; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11932749
Comments: 5' arm is 4.3 kb. 3' arm is 3.0 kb. See author's map for details.
Proper citation: RRID:Addgene_16648 Copy
Species: Homo sapiens
Genetic Insert: hU6-gRNA and hH1-gRNA targeting cldn1 gene
Vector Backbone Description: Backbone Marker:Addgene #122182; Vector Backbone:pLenti spCas9 T2A iRFP670 P2A puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35388524
Proper citation: RRID:Addgene_166133 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4856; Vector Backbone:pFastBac HT; Vector Types:Mammalian Expression, Baculoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15016963
Comments: A fragment of human PIK3CA cDNA including the entire ORF was cloned into the pFastBac HT vector. The cDNA fragment can be removed with BamHI and HindIII for subcloning into other vectors.
Proper citation: RRID:Addgene_16644 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA H1047R
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4856; Vector Backbone:pFastBac HT; Vector Types:Mammalian Expression, Baculoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15016963
Comments: A fragment of human PIK3CA cDNA including the entire ORF was cloned into the pFastBac HT vector. The cDNA fragment can be removed with BamHI and HindIII for subcloning into other vectors.
Proper citation: RRID:Addgene_16641 Copy
Genetic Insert: [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33727557
Proper citation: RRID:Addgene_166246 Copy
Species: Homo sapiens
Genetic Insert: shRNA for human YAP
Vector Backbone Description: Vector Backbone:PLKO.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166485 Copy
Species: Homo sapiens
Genetic Insert: DNMT3b knock-out construct
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3264; Vector Backbone:pSL301; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11932749
Comments: 5' arm is 3.0 kb. 3' arm is 4.6 kb. See author's map for details.
Proper citation: RRID:Addgene_16651 Copy
Species: Homo sapiens
Genetic Insert: DNMT3b knock-out construct
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3264; Vector Backbone:pSL301; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11932749
Comments: 5' arm is 3.0 kb. 3' arm is 4.6 kb. See author's map for details.
Proper citation: RRID:Addgene_16650 Copy
Species: Mus musculus
Genetic Insert: gRNA for mouse Yap
Vector Backbone Description: Vector Backbone:Lenti-CRISPR-V2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166482 Copy
Species: Mus musculus
Genetic Insert: gRNA for mouse Yap
Vector Backbone Description: Vector Backbone:Lenti-CRISPR-V2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166481 Copy
Species: Homo sapiens
Genetic Insert: hPOT1
Vector Backbone Description: Backbone Size:5665; Vector Backbone:pWZL N-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33122293
Proper citation: RRID:Addgene_166407 Copy
Species: Homo sapiens
Genetic Insert: hPOT1
Vector Backbone Description: Backbone Size:5665; Vector Backbone:pWZL N-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33122293
Proper citation: RRID:Addgene_166406 Copy
Species: Homo sapiens
Genetic Insert: BUB1 S492Y
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:5099; Vector Backbone:pBI-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9521327
Comments: pBI-GFP-Bub1 S492Y was constructed by cloning RT-PCR products from tumour line V429 into the NotI and SalI sites of pBI-GFP.
Proper citation: RRID:Addgene_16625 Copy
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