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Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167680 Copy
Species: Streptococcus pyogenes
Genetic Insert: PcUB4-2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: in addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167678 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB402; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141301 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141302 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Aashima Khosla, David Nelson (unpublished); Backbone Size:13299; Vector Backbone:pRATIO1212; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141311 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Aashima Khosla, David Nelson (unpublished); Backbone Size:14000; Vector Backbone:pRATIO2214; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141314 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10844; Vector Backbone:pGWB401; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141285 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11165; Vector Backbone:pGWB501; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141286 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10700; Vector Backbone:pGWB601; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141287 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB402; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141299 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141296 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153229 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153228 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153233 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153232 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153231 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153237 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153236 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153235 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFRm43GW; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_133748 Copy
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