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Species: Saccharomyces cerevisiae
Genetic Insert: CUP1-1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrVIII:212613..212632 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166086 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET16
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXVI:877523..877542 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome.
Addgene QC identified several discrepancies compared to the theoretical reference.
Proper citation: RRID:Addgene_166091 Copy
Genetic Insert: N/A
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:33511418
Comments: Genotype: MG1655 rph+ ΔlacIZYA ΔλattB::lacO-_λattB_PlacUV5.lacO2_LacZ (pIT3-SH-pLacI-LacI reverse)φHK022attB
This strain contains the pInt-ts lambda helper plasmid (Addgene #66076) necessary for the genome integration of pLOM2-500 plasmid (Addgene #164868) or its derivative. This strain expresses lac repressor.
Proper citation: RRID:Addgene_166037 Copy
Genetic Insert: N/A
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:33511418
Comments: Genotype: MG1655 rph+ ΔlacIZYA ΔλattB::lacOid_λattB_PlacUV5.lacO2_LacZ (pIT3-SH)φHK022attB
This strain contains the pInt-ts lambda helper plasmid (Addgene #66076) necessary for the genome integration of pLOM2-500 plasmid (Addgene #164868) or its derivative. This strain does not express lac repressor.
Proper citation: RRID:Addgene_166036 Copy
Species: Homo sapiens
Genetic Insert: PFAS-mCherry
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32439803
Proper citation: RRID:Addgene_166024 Copy
Species: Mus musculus
Genetic Insert: GCaMP6f with 4 miR-128 binding sites
Vector Backbone Description: Vector Backbone:AAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33158963
Comments: Please note that 3' ITR is not annotated on Addgene's plasmid map, however the plasmid can still be packaged and functions as expected.
Proper citation: RRID:Addgene_166023 Copy
Species: Pseudomonas protegens
Genetic Insert: phlF
Vector Backbone Description: Vector Backbone:pRS405red; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Proper citation: RRID:Addgene_166029 Copy
Species: Homo sapiens
Genetic Insert: human equilibrative nucleoside transporter 1
Vector Backbone Description: Vector Backbone:pGK401red; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Comments: hENT1 gene was cloned from addgene plasmid pFS181 (#12536, Depositor: Nick Rhind)
Proper citation: RRID:Addgene_166028 Copy
Species: Other
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166020 Copy
Species: Homo sapiens
Genetic Insert: c-myc promoter (Frag B)
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9727977
Comments: See Author's Map and article for more information.
Proper citation: RRID:Addgene_16606 Copy
Species: Homo sapiens
Genetic Insert: FDXR knockout
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: See author's map for more information. 5' arm is 0.8 kb (-768 to -1) and 3' arm is 3.4 kb (+2051 to +5336).
Proper citation: RRID:Addgene_16613 Copy
Species: Homo sapiens
Genetic Insert: MnSOD
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5200; Vector Backbone:pBI-EGFP2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: Contains full length MnSOD gene including the mitochondrial leader sequence.
Proper citation: RRID:Addgene_16612 Copy
Species: Homo sapiens
Genetic Insert: FDXR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pTRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: See author's map for cloning details.
Proper citation: RRID:Addgene_16611 Copy
Species: Homo sapiens
Genetic Insert: FDXR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pTRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: See author's map for cloning details.
Proper citation: RRID:Addgene_16610 Copy
Species: Mus musculus
Genetic Insert: I392N-Talin-1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4734; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, CMV promoter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33431906
Proper citation: RRID:Addgene_166114 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of RPL22A
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXII:263006..263025 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome.
Addgene QC identified discrepancies compared to the theoretical sequence. The depositing lab confirms the functional activity of the construct despite these differences.
Proper citation: RRID:Addgene_166079 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of RPL39 (Overlaps with 5'UTR and first base of gene)
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrX: 75914-75933 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166078 Copy
Species: Mus musculus
Genetic Insert: R1368W-talin1-Talin-1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4734; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, CMV promoter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33431906
Proper citation: RRID:Addgene_166117 Copy
Species: Mus musculus
Genetic Insert: A893E-Talin-1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4734; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, CMV promoter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33431906
Proper citation: RRID:Addgene_166116 Copy
Species: Mus musculus
Genetic Insert: V577D-Talin-1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4734; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, CMV promoter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33431906
Proper citation: RRID:Addgene_166115 Copy
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